Drifter technique: a new method to obtain metaphases in Hep-2 cell line cultures

The Hep-2 cell line is derived from laryngeal carcinoma cells and is often utilized as a model in carcinogenesis and mutagenesis tests. To evaluate the proliferative potential of this line, we developed a cytogenetic methodology (drifter technique) to obtain metaphases from cells that loose cellular adhesion when they underwent mitosis in culture. By this procedure, 2000 cells were counted, resulting in a mitotic index (MI) of 22.2%. Although this MI was not statistically different from the one obtained using either a classical cytogenetic method or a cell synchronization technique, the drifter technique has the advantage of not requiring the use of some reagents for the obtention of metaphases and also of diminishing the consumption of maintenance reagents for this cell line.

Bibliographic Details

Main Authors:	Lima,Eleonidas Moura, Rissino,Jorge Dores, Guimarães,Adriana Costa, Overal,David James, Khayat,André Salim, Souza,Patrícia Carvalho de, Christofolini,Denise Maria, Smith,Marília de Arruda Cardoso, Burbano,Rommel Rodríguez
Format:	Digital revista
Language:	English
Published:	Instituto de Tecnologia do Paraná - Tecpar 2005
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000500005
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