DNA typing from vaginal smear slides in suspected rape cases
In an investigation of suspected rape, proof of sexual assault with penetration is required. In view of this, detailed descriptions of the genitalia, the thighs and pubic region are made within the forensic medical service. In addition, vaginal swabs are taken from the rape victim and some of the biological material collected is then transferred to glass slides. In this report, we describe two rape cases solved using DNA typing from cells recovered from vaginal smear slides. In 1999, two young women informed the Rio de Janeiro Police Department that they had been victims of sexual assaults. A suspect was arrested and the victims identified him as the offender. The suspect maintained that he was innocent. In order to elucidate these crimes, vaginal smear slides were sent to the DNA Diagnostic Laboratory for DNA analysis three months after the crimes, as unique forensic evidence. To get enough epithelial and sperm cells to perform DNA analysis, we used protocols modified from the previously standard protocols used for DNA extraction from biological material fixed on glass slides. The quantity of cells was sufficient to perform human DNA typing using nine short tandem repeat (STR) loci. It was 3.3 billion times more probable that it was the examined suspect who had left sperm cells in the victims, rather than any other individual in the population of Rio de Janeiro.
Main Authors: | Silva,Dayse Aparecida da, Góes,Andréa Carla de Souza, Carvalho,Jorge José de, Carvalho,Elizeu Fagundes de |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Associação Paulista de Medicina - APM
2004
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-31802004000200008 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Identification of a criminal by DNA typing in a rape case in Rio de Janeiro, Brazil
by: Góes,Andréa Carla de Souza, et al.
Published: (2002) -
Stained smears as a source of DNA
by: Alger,Jackeline, et al.
Published: (1996) -
Alternative method of smearing samples on slides facilitate visualization of hemoglobin H inclusion bodies in alpha thalassemia
by: Mendiburu,Carlos F., et al.
Published: (2008) -
Detection of GBV-C/HGV RNA in cervico-vaginal smears from healthy individuals
by: Watanabe,Maria Angelica Ehara, et al.
Published: (2008) -
Recovering of DNA evidence after rape
by: Jina,Ruxana, et al.
Published: (2011)