DNA typing from vaginal smear slides in suspected rape cases

DNA typing from vaginal smear slides in suspected rape cases

In an investigation of suspected rape, proof of sexual assault with penetration is required. In view of this, detailed descriptions of the genitalia, the thighs and pubic region are made within the forensic medical service. In addition, vaginal swabs are taken from the rape victim and some of the biological material collected is then transferred to glass slides. In this report, we describe two rape cases solved using DNA typing from cells recovered from vaginal smear slides. In 1999, two young women informed the Rio de Janeiro Police Department that they had been victims of sexual assaults. A suspect was arrested and the victims identified him as the offender. The suspect maintained that he was innocent. In order to elucidate these crimes, vaginal smear slides were sent to the DNA Diagnostic Laboratory for DNA analysis three months after the crimes, as unique forensic evidence. To get enough epithelial and sperm cells to perform DNA analysis, we used protocols modified from the previously standard protocols used for DNA extraction from biological material fixed on glass slides. The quantity of cells was sufficient to perform human DNA typing using nine short tandem repeat (STR) loci. It was 3.3 billion times more probable that it was the examined suspect who had left sperm cells in the victims, rather than any other individual in the population of Rio de Janeiro.

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Auteurs principaux: Silva,Dayse Aparecida da, Góes,Andréa Carla de Souza, Carvalho,Jorge José de, Carvalho,Elizeu Fagundes de
Format: Digital revista
Langue:English
Publié: Associação Paulista de Medicina - APM 2004
Accès en ligne:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-31802004000200008
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spelling oai:scielo:S1516-318020040002000082004-07-05DNA typing from vaginal smear slides in suspected rape casesSilva,Dayse Aparecida daGóes,Andréa Carla de SouzaCarvalho,Jorge José deCarvalho,Elizeu Fagundes de DNA Profile Rape Forensic sciences Vaginal smears In an investigation of suspected rape, proof of sexual assault with penetration is required. In view of this, detailed descriptions of the genitalia, the thighs and pubic region are made within the forensic medical service. In addition, vaginal swabs are taken from the rape victim and some of the biological material collected is then transferred to glass slides. In this report, we describe two rape cases solved using DNA typing from cells recovered from vaginal smear slides. In 1999, two young women informed the Rio de Janeiro Police Department that they had been victims of sexual assaults. A suspect was arrested and the victims identified him as the offender. The suspect maintained that he was innocent. In order to elucidate these crimes, vaginal smear slides were sent to the DNA Diagnostic Laboratory for DNA analysis three months after the crimes, as unique forensic evidence. To get enough epithelial and sperm cells to perform DNA analysis, we used protocols modified from the previously standard protocols used for DNA extraction from biological material fixed on glass slides. The quantity of cells was sufficient to perform human DNA typing using nine short tandem repeat (STR) loci. It was 3.3 billion times more probable that it was the examined suspect who had left sperm cells in the victims, rather than any other individual in the population of Rio de Janeiro.info:eu-repo/semantics/openAccessAssociação Paulista de Medicina - APMSao Paulo Medical Journal v.122 n.2 20042004-04-01info:eu-repo/semantics/othertext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-31802004000200008en10.1590/S1516-31802004000200008
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region America del Sur
libraryname SciELO
language English
format Digital
author Silva,Dayse Aparecida da
Góes,Andréa Carla de Souza
Carvalho,Jorge José de
Carvalho,Elizeu Fagundes de
spellingShingle Silva,Dayse Aparecida da
Góes,Andréa Carla de Souza
Carvalho,Jorge José de
Carvalho,Elizeu Fagundes de
DNA typing from vaginal smear slides in suspected rape cases
author_facet Silva,Dayse Aparecida da
Góes,Andréa Carla de Souza
Carvalho,Jorge José de
Carvalho,Elizeu Fagundes de
author_sort Silva,Dayse Aparecida da
title DNA typing from vaginal smear slides in suspected rape cases
title_short DNA typing from vaginal smear slides in suspected rape cases
title_full DNA typing from vaginal smear slides in suspected rape cases
title_fullStr DNA typing from vaginal smear slides in suspected rape cases
title_full_unstemmed DNA typing from vaginal smear slides in suspected rape cases
title_sort dna typing from vaginal smear slides in suspected rape cases
description In an investigation of suspected rape, proof of sexual assault with penetration is required. In view of this, detailed descriptions of the genitalia, the thighs and pubic region are made within the forensic medical service. In addition, vaginal swabs are taken from the rape victim and some of the biological material collected is then transferred to glass slides. In this report, we describe two rape cases solved using DNA typing from cells recovered from vaginal smear slides. In 1999, two young women informed the Rio de Janeiro Police Department that they had been victims of sexual assaults. A suspect was arrested and the victims identified him as the offender. The suspect maintained that he was innocent. In order to elucidate these crimes, vaginal smear slides were sent to the DNA Diagnostic Laboratory for DNA analysis three months after the crimes, as unique forensic evidence. To get enough epithelial and sperm cells to perform DNA analysis, we used protocols modified from the previously standard protocols used for DNA extraction from biological material fixed on glass slides. The quantity of cells was sufficient to perform human DNA typing using nine short tandem repeat (STR) loci. It was 3.3 billion times more probable that it was the examined suspect who had left sperm cells in the victims, rather than any other individual in the population of Rio de Janeiro.
publisher Associação Paulista de Medicina - APM
publishDate 2004
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-31802004000200008
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