Specific amplification of iron receptor genes in Xylella fastidiosa strains from different hosts
Bacterial production of siderophores may involve specific genes related to nonribosomal peptide and polyketide biosynthesis, which have not been fully identified in the genome of Xylella fastidiosa strain 9a5c. However, a search for siderophore-related genes in strain 9a5c indicated five membrane receptors, including siderophore, ferrichrome-iron and hemin receptors. All these biomolecules are thought to be associated with iron transport and utilization. Eighty isolates obtained from citrus orchards containing trees that developed citrus variegated chlorosis (CVC) were screened for siderophore production. The results demonstrated that only 10 of the isolates did not produce siderophores. Additional strains obtained from coffee, almond, mulberry, elm, ragweed, periwinkle and grape also infected by X. fastidiosa were also shown by the chromeazurol bioassay to produce siderophores. In order to correlate siderophore production with the presence of siderophore-related genes, a polymerase chain reaction (PCR) was developed using specific primers for the catechol-type ferric enterobactin receptor (pfeA) and the hydroxamate-type ferrisiderophore receptor (fiuA) genes of strain 9a5c. The PCR results confirmed our hypothesis by demonstrating that amplification products were detected in all strains except for those isolates that did not produce siderophores.
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Sociedade Brasileira de Genética
2006
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oai:scielo:S1415-475720060001000262006-03-10Specific amplification of iron receptor genes in Xylella fastidiosa strains from different hostsPacheco,Flávia Teresa HansenSilva-Stenico,Maria EstelaEtchegaray,AugustoGomes,José EliasCarrilho,EmanuelTsai,Siu Mui citrus variegated chlorosis plant pathogen iron-transportation pyoverdine enterobactin polyketide synthase peptide synthetase Bacterial production of siderophores may involve specific genes related to nonribosomal peptide and polyketide biosynthesis, which have not been fully identified in the genome of Xylella fastidiosa strain 9a5c. However, a search for siderophore-related genes in strain 9a5c indicated five membrane receptors, including siderophore, ferrichrome-iron and hemin receptors. All these biomolecules are thought to be associated with iron transport and utilization. Eighty isolates obtained from citrus orchards containing trees that developed citrus variegated chlorosis (CVC) were screened for siderophore production. The results demonstrated that only 10 of the isolates did not produce siderophores. Additional strains obtained from coffee, almond, mulberry, elm, ragweed, periwinkle and grape also infected by X. fastidiosa were also shown by the chromeazurol bioassay to produce siderophores. In order to correlate siderophore production with the presence of siderophore-related genes, a polymerase chain reaction (PCR) was developed using specific primers for the catechol-type ferric enterobactin receptor (pfeA) and the hydroxamate-type ferrisiderophore receptor (fiuA) genes of strain 9a5c. The PCR results confirmed our hypothesis by demonstrating that amplification products were detected in all strains except for those isolates that did not produce siderophores.info:eu-repo/semantics/openAccessSociedade Brasileira de GenéticaGenetics and Molecular Biology v.29 n.1 20062006-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100026en10.1590/S1415-47572006000100026 |
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Pacheco,Flávia Teresa Hansen Silva-Stenico,Maria Estela Etchegaray,Augusto Gomes,José Elias Carrilho,Emanuel Tsai,Siu Mui |
spellingShingle |
Pacheco,Flávia Teresa Hansen Silva-Stenico,Maria Estela Etchegaray,Augusto Gomes,José Elias Carrilho,Emanuel Tsai,Siu Mui Specific amplification of iron receptor genes in Xylella fastidiosa strains from different hosts |
author_facet |
Pacheco,Flávia Teresa Hansen Silva-Stenico,Maria Estela Etchegaray,Augusto Gomes,José Elias Carrilho,Emanuel Tsai,Siu Mui |
author_sort |
Pacheco,Flávia Teresa Hansen |
title |
Specific amplification of iron receptor genes in Xylella fastidiosa strains from different hosts |
title_short |
Specific amplification of iron receptor genes in Xylella fastidiosa strains from different hosts |
title_full |
Specific amplification of iron receptor genes in Xylella fastidiosa strains from different hosts |
title_fullStr |
Specific amplification of iron receptor genes in Xylella fastidiosa strains from different hosts |
title_full_unstemmed |
Specific amplification of iron receptor genes in Xylella fastidiosa strains from different hosts |
title_sort |
specific amplification of iron receptor genes in xylella fastidiosa strains from different hosts |
description |
Bacterial production of siderophores may involve specific genes related to nonribosomal peptide and polyketide biosynthesis, which have not been fully identified in the genome of Xylella fastidiosa strain 9a5c. However, a search for siderophore-related genes in strain 9a5c indicated five membrane receptors, including siderophore, ferrichrome-iron and hemin receptors. All these biomolecules are thought to be associated with iron transport and utilization. Eighty isolates obtained from citrus orchards containing trees that developed citrus variegated chlorosis (CVC) were screened for siderophore production. The results demonstrated that only 10 of the isolates did not produce siderophores. Additional strains obtained from coffee, almond, mulberry, elm, ragweed, periwinkle and grape also infected by X. fastidiosa were also shown by the chromeazurol bioassay to produce siderophores. In order to correlate siderophore production with the presence of siderophore-related genes, a polymerase chain reaction (PCR) was developed using specific primers for the catechol-type ferric enterobactin receptor (pfeA) and the hydroxamate-type ferrisiderophore receptor (fiuA) genes of strain 9a5c. The PCR results confirmed our hypothesis by demonstrating that amplification products were detected in all strains except for those isolates that did not produce siderophores. |
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Sociedade Brasileira de Genética |
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2006 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100026 |
work_keys_str_mv |
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