Receptiveness of the stigma and in vitro germination of orange pollen, submitted to different temperatures

The study was carried out in order to evaluate the effect of temperature and in vitor stigma receptivity on regeneration of orange cultivar (Valência, Pêra and Natal) pollen. Two experiments were carried out, in the first the ideal temperature of germination was assessed. Pollen was obtained from flowers at the balloon stage and inoculated in culture medium with 10 g L-1 agar, 200 mg L-1 boric acid, 100 g L-1 sucrose, 800 mg L-1 calcium nitrate, pH adjusted to 6,5 and incubated in a BOD chamber at temperatures of 23, 24, 25, 26 and 27ºC during a 24-hour photoperiod. After 12 hours of incubation, the best temperature for pollen grain germination was 25ºC for all varieties. In a second experiment, in order to test the receptivity of the stigma, flowers were collected at different flowering stages: small bud, balloon and open flower. The stigmas were by immersion exposed to hydrogen peroxide (perioxidase reaction), 3% for 3 minutes. Through the technique of Zeisler (1938), better results were detected at the balloon stage with 80 to 100% receptivity, while for the open flower the receptivity presented maximum indexes.

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Main Authors: Pio,Leila Aparecida Salles, Ramos,José Darlan, Pasqual,Moacir, Santos,Flavia Carvalho, Junqueira,Keize Pereira
Format: Digital revista
Language:English
Published: Editora da UFLA 2004
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542004000500016
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spelling oai:scielo:S1413-705420040005000162010-09-29Receptiveness of the stigma and in vitro germination of orange pollen, submitted to different temperaturesPio,Leila Aparecida SallesRamos,José DarlanPasqual,MoacirSantos,Flavia CarvalhoJunqueira,Keize Pereira Citrus sinensis Palinology tissue culture The study was carried out in order to evaluate the effect of temperature and in vitor stigma receptivity on regeneration of orange cultivar (Valência, Pêra and Natal) pollen. Two experiments were carried out, in the first the ideal temperature of germination was assessed. Pollen was obtained from flowers at the balloon stage and inoculated in culture medium with 10 g L-1 agar, 200 mg L-1 boric acid, 100 g L-1 sucrose, 800 mg L-1 calcium nitrate, pH adjusted to 6,5 and incubated in a BOD chamber at temperatures of 23, 24, 25, 26 and 27ºC during a 24-hour photoperiod. After 12 hours of incubation, the best temperature for pollen grain germination was 25ºC for all varieties. In a second experiment, in order to test the receptivity of the stigma, flowers were collected at different flowering stages: small bud, balloon and open flower. The stigmas were by immersion exposed to hydrogen peroxide (perioxidase reaction), 3% for 3 minutes. Through the technique of Zeisler (1938), better results were detected at the balloon stage with 80 to 100% receptivity, while for the open flower the receptivity presented maximum indexes.info:eu-repo/semantics/openAccessEditora da UFLACiência e Agrotecnologia v.28 n.5 20042004-10-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542004000500016en10.1590/S1413-70542004000500016
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Pio,Leila Aparecida Salles
Ramos,José Darlan
Pasqual,Moacir
Santos,Flavia Carvalho
Junqueira,Keize Pereira
spellingShingle Pio,Leila Aparecida Salles
Ramos,José Darlan
Pasqual,Moacir
Santos,Flavia Carvalho
Junqueira,Keize Pereira
Receptiveness of the stigma and in vitro germination of orange pollen, submitted to different temperatures
author_facet Pio,Leila Aparecida Salles
Ramos,José Darlan
Pasqual,Moacir
Santos,Flavia Carvalho
Junqueira,Keize Pereira
author_sort Pio,Leila Aparecida Salles
title Receptiveness of the stigma and in vitro germination of orange pollen, submitted to different temperatures
title_short Receptiveness of the stigma and in vitro germination of orange pollen, submitted to different temperatures
title_full Receptiveness of the stigma and in vitro germination of orange pollen, submitted to different temperatures
title_fullStr Receptiveness of the stigma and in vitro germination of orange pollen, submitted to different temperatures
title_full_unstemmed Receptiveness of the stigma and in vitro germination of orange pollen, submitted to different temperatures
title_sort receptiveness of the stigma and in vitro germination of orange pollen, submitted to different temperatures
description The study was carried out in order to evaluate the effect of temperature and in vitor stigma receptivity on regeneration of orange cultivar (Valência, Pêra and Natal) pollen. Two experiments were carried out, in the first the ideal temperature of germination was assessed. Pollen was obtained from flowers at the balloon stage and inoculated in culture medium with 10 g L-1 agar, 200 mg L-1 boric acid, 100 g L-1 sucrose, 800 mg L-1 calcium nitrate, pH adjusted to 6,5 and incubated in a BOD chamber at temperatures of 23, 24, 25, 26 and 27ºC during a 24-hour photoperiod. After 12 hours of incubation, the best temperature for pollen grain germination was 25ºC for all varieties. In a second experiment, in order to test the receptivity of the stigma, flowers were collected at different flowering stages: small bud, balloon and open flower. The stigmas were by immersion exposed to hydrogen peroxide (perioxidase reaction), 3% for 3 minutes. Through the technique of Zeisler (1938), better results were detected at the balloon stage with 80 to 100% receptivity, while for the open flower the receptivity presented maximum indexes.
publisher Editora da UFLA
publishDate 2004
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542004000500016
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