Nontuberculous mycobacteria from mexican archaeological sites
Abstract We examined several buildings of nine archaeological sites in Mexico for the presence of mycobacteria and we could isolate forty-five nontuberculous mycobacteria (NTM). These were isolated from biofilms using selective media containing different antibiotics and dyes. Identification of the isolated mycobacteria was carried out, first, by a molecular identification by means of a mycobacteria-specific PCR using bacterial lysates of the acid-fast bacilli followed by species identification by comparing of three molecular markers: genes rrs (16SrRNA), hsp65 and rpoB. Furthermore, the physiographic data of the archaeological zones under study was related to the number of acid-fast microorganisms using a univariate analysis of variance. From the 45 isolated mycobacteria, 21 were Mycobacteroides chelonae; seven, Mycobacteroides abscessus; five, Mycolicibacterium flavescens; four, Mycobacterium alvei; two, Mycobacterium fortuitum; and six, Mycobacterium sp. Most NTM were isolated from two archaeological sites: 25 from Guachimontones (Jalisco), and 13 from Atetelco (Estado de México). The statistical analysis showed that environmental factors such as climate and the temperature-humidity-precipitation interaction had the greatest influence on the presence of NTM in these archaeological zones.
| Main Authors: | , , , |
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| Format: | Digital revista |
| Language: | English |
| Published: |
Universidad Nacional Autónoma de México, Facultad de Estudios Superiores Zaragoza
2019
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| Online Access: | http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-888X2019000100108 |
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