Herpes simplex virus: isolation, cytopathological characterization and antiviral sensitivity
BACKGROUND:Herpes simplex virus (HSV) infection is an endemic disease and it is estimated that 6095% of the adult population are infected with symptoms that are usually self-limiting, though they can be serious, extensive and prolonged in immunocompromised individuals, highlighted by the emergence of drug-resistant strains. The study of the wild-type HSV strains based on the cytopathogenic features and its antiviral sensitivity are important in the establishment of an antivirogram for controlling the infection.OBJECTIVE:This study sought to isolate and examine the cytopathological characteristics of circulating strains of the Herpes simplex virus, from clinical specimens and their sensitivity to commercially available antiherpesvirus drugs, acyclovir, phosphonophormic acid and trifluridine.METHODS:Herpes simplex virus isolation, cytopathological features and antiviral sensitivity assays were performed in cell culture by tissue culture infectious dose or plaque forming unit assay.RESULTS:From twenty-two clinical specimens, we isolated and adapted nine strains. Overall, the cytopathic effect was detected 24 h post-infection (p.i.) and the presence of syncytia was remarkable 48 h p.i., observed after cell staining. Out of eight isolates, four developed plaques of varying sizes. All the isolates were sensitive to acyclovir, phosphonophormic and trifluridine, with the percentage of virus inhibition (%VI) ranging from 49.7-100%.CONCLUSIONS:The methodology for HSV isolation and characterization is a straightforward approach, but the drug sensitivity test, regarded as being of great practical importance, needs to be better understood.
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Sociedade Brasileira de Dermatologia
2014
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oai:scielo:S0365-059620140003004482015-10-19Herpes simplex virus: isolation, cytopathological characterization and antiviral sensitivityNozawa,CarlosHattori,Lilian YumiGalhardi,Ligia Carla FaccinLopes,NayaraBomfim,Wesley AndradeCândido,Ligyana Korki deAzevedo,Elbens Marcos Minoreli deGon,Airton dos SantosLinhares,Rosa Elisa Carvalho Antiviral agents Herpesvirus 1, human Herpesvirus 2, human BACKGROUND:Herpes simplex virus (HSV) infection is an endemic disease and it is estimated that 6095% of the adult population are infected with symptoms that are usually self-limiting, though they can be serious, extensive and prolonged in immunocompromised individuals, highlighted by the emergence of drug-resistant strains. The study of the wild-type HSV strains based on the cytopathogenic features and its antiviral sensitivity are important in the establishment of an antivirogram for controlling the infection.OBJECTIVE:This study sought to isolate and examine the cytopathological characteristics of circulating strains of the Herpes simplex virus, from clinical specimens and their sensitivity to commercially available antiherpesvirus drugs, acyclovir, phosphonophormic acid and trifluridine.METHODS:Herpes simplex virus isolation, cytopathological features and antiviral sensitivity assays were performed in cell culture by tissue culture infectious dose or plaque forming unit assay.RESULTS:From twenty-two clinical specimens, we isolated and adapted nine strains. Overall, the cytopathic effect was detected 24 h post-infection (p.i.) and the presence of syncytia was remarkable 48 h p.i., observed after cell staining. Out of eight isolates, four developed plaques of varying sizes. All the isolates were sensitive to acyclovir, phosphonophormic and trifluridine, with the percentage of virus inhibition (%VI) ranging from 49.7-100%.CONCLUSIONS:The methodology for HSV isolation and characterization is a straightforward approach, but the drug sensitivity test, regarded as being of great practical importance, needs to be better understood.info:eu-repo/semantics/openAccessSociedade Brasileira de DermatologiaAnais Brasileiros de Dermatologia v.89 n.3 20142014-06-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0365-05962014000300448en10.1590/abd1806-4841.20142574 |
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Nozawa,Carlos Hattori,Lilian Yumi Galhardi,Ligia Carla Faccin Lopes,Nayara Bomfim,Wesley Andrade Cândido,Ligyana Korki de Azevedo,Elbens Marcos Minoreli de Gon,Airton dos Santos Linhares,Rosa Elisa Carvalho |
spellingShingle |
Nozawa,Carlos Hattori,Lilian Yumi Galhardi,Ligia Carla Faccin Lopes,Nayara Bomfim,Wesley Andrade Cândido,Ligyana Korki de Azevedo,Elbens Marcos Minoreli de Gon,Airton dos Santos Linhares,Rosa Elisa Carvalho Herpes simplex virus: isolation, cytopathological characterization and antiviral sensitivity |
author_facet |
Nozawa,Carlos Hattori,Lilian Yumi Galhardi,Ligia Carla Faccin Lopes,Nayara Bomfim,Wesley Andrade Cândido,Ligyana Korki de Azevedo,Elbens Marcos Minoreli de Gon,Airton dos Santos Linhares,Rosa Elisa Carvalho |
author_sort |
Nozawa,Carlos |
title |
Herpes simplex virus: isolation, cytopathological characterization and antiviral sensitivity |
title_short |
Herpes simplex virus: isolation, cytopathological characterization and antiviral sensitivity |
title_full |
Herpes simplex virus: isolation, cytopathological characterization and antiviral sensitivity |
title_fullStr |
Herpes simplex virus: isolation, cytopathological characterization and antiviral sensitivity |
title_full_unstemmed |
Herpes simplex virus: isolation, cytopathological characterization and antiviral sensitivity |
title_sort |
herpes simplex virus: isolation, cytopathological characterization and antiviral sensitivity |
description |
BACKGROUND:Herpes simplex virus (HSV) infection is an endemic disease and it is estimated that 6095% of the adult population are infected with symptoms that are usually self-limiting, though they can be serious, extensive and prolonged in immunocompromised individuals, highlighted by the emergence of drug-resistant strains. The study of the wild-type HSV strains based on the cytopathogenic features and its antiviral sensitivity are important in the establishment of an antivirogram for controlling the infection.OBJECTIVE:This study sought to isolate and examine the cytopathological characteristics of circulating strains of the Herpes simplex virus, from clinical specimens and their sensitivity to commercially available antiherpesvirus drugs, acyclovir, phosphonophormic acid and trifluridine.METHODS:Herpes simplex virus isolation, cytopathological features and antiviral sensitivity assays were performed in cell culture by tissue culture infectious dose or plaque forming unit assay.RESULTS:From twenty-two clinical specimens, we isolated and adapted nine strains. Overall, the cytopathic effect was detected 24 h post-infection (p.i.) and the presence of syncytia was remarkable 48 h p.i., observed after cell staining. Out of eight isolates, four developed plaques of varying sizes. All the isolates were sensitive to acyclovir, phosphonophormic and trifluridine, with the percentage of virus inhibition (%VI) ranging from 49.7-100%.CONCLUSIONS:The methodology for HSV isolation and characterization is a straightforward approach, but the drug sensitivity test, regarded as being of great practical importance, needs to be better understood. |
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Sociedade Brasileira de Dermatologia |
publishDate |
2014 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0365-05962014000300448 |
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