Genetic divergence among hybrids of 'Cravo' mandarin with 'Pêra' sweet orange
Molecular markers have been used as tools in breading programs of sexual hybridation, allowing the genetic characterization of a large number of genotypes. The RADP markers are the most used since the employed techniques are simple and of low cost. To evaluate the genetic divergence among F1 hybrids of 'Cravo' mandarin (Citrus reticulata Blanco) and 'Pêra' sweet orange (C. sinensis (L.) Osbeck), this study analyses the variability and similarity of the hybrids among themselves and with their parents. Random Amplified Polimorfic DNA marker analysis, with 102 primers, were applied to a population composed of 94 hybrids and their parents. Multivariate genetic divergence analysis of the principal components and Tocher grouping were carried out only considering the polymorphic fragments. Genetic distances were calculated by the arithmetic complement of the Jaccard index. Bidimensional dispersion graphs among hybrid and parent distances and of the divergence analysis by principal components were constructed. High genetic similarity among Cravo and Pêra varieties and their hybrids was verified, showing a casual distribution from the hybrids in relation to the parents, but in intermediary positions. The principal component analysis showed little applicability in the study of hybrid genetic divergence. The hybrids and parents were classified in groups based on the genetic similarity, using the Tocher optimization method.
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Escola Superior de Agricultura "Luiz de Queiroz"
2003
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oai:scielo:S0103-901620030001000172003-03-20Genetic divergence among hybrids of 'Cravo' mandarin with 'Pêra' sweet orangeOliveira,Roberto Pedroso deAguilar-Vildoso,Carlos IvanMachado,Marcos Antônio Citrus reticulata Citrus sinensis Tocher grouping RAPD citrus Molecular markers have been used as tools in breading programs of sexual hybridation, allowing the genetic characterization of a large number of genotypes. The RADP markers are the most used since the employed techniques are simple and of low cost. To evaluate the genetic divergence among F1 hybrids of 'Cravo' mandarin (Citrus reticulata Blanco) and 'Pêra' sweet orange (C. sinensis (L.) Osbeck), this study analyses the variability and similarity of the hybrids among themselves and with their parents. Random Amplified Polimorfic DNA marker analysis, with 102 primers, were applied to a population composed of 94 hybrids and their parents. Multivariate genetic divergence analysis of the principal components and Tocher grouping were carried out only considering the polymorphic fragments. Genetic distances were calculated by the arithmetic complement of the Jaccard index. Bidimensional dispersion graphs among hybrid and parent distances and of the divergence analysis by principal components were constructed. High genetic similarity among Cravo and Pêra varieties and their hybrids was verified, showing a casual distribution from the hybrids in relation to the parents, but in intermediary positions. The principal component analysis showed little applicability in the study of hybrid genetic divergence. The hybrids and parents were classified in groups based on the genetic similarity, using the Tocher optimization method.info:eu-repo/semantics/openAccessEscola Superior de Agricultura "Luiz de Queiroz"Scientia Agricola v.60 n.1 20032003-02-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000100017en10.1590/S0103-90162003000100017 |
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Oliveira,Roberto Pedroso de Aguilar-Vildoso,Carlos Ivan Machado,Marcos Antônio |
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Oliveira,Roberto Pedroso de Aguilar-Vildoso,Carlos Ivan Machado,Marcos Antônio Genetic divergence among hybrids of 'Cravo' mandarin with 'Pêra' sweet orange |
author_facet |
Oliveira,Roberto Pedroso de Aguilar-Vildoso,Carlos Ivan Machado,Marcos Antônio |
author_sort |
Oliveira,Roberto Pedroso de |
title |
Genetic divergence among hybrids of 'Cravo' mandarin with 'Pêra' sweet orange |
title_short |
Genetic divergence among hybrids of 'Cravo' mandarin with 'Pêra' sweet orange |
title_full |
Genetic divergence among hybrids of 'Cravo' mandarin with 'Pêra' sweet orange |
title_fullStr |
Genetic divergence among hybrids of 'Cravo' mandarin with 'Pêra' sweet orange |
title_full_unstemmed |
Genetic divergence among hybrids of 'Cravo' mandarin with 'Pêra' sweet orange |
title_sort |
genetic divergence among hybrids of 'cravo' mandarin with 'pêra' sweet orange |
description |
Molecular markers have been used as tools in breading programs of sexual hybridation, allowing the genetic characterization of a large number of genotypes. The RADP markers are the most used since the employed techniques are simple and of low cost. To evaluate the genetic divergence among F1 hybrids of 'Cravo' mandarin (Citrus reticulata Blanco) and 'Pêra' sweet orange (C. sinensis (L.) Osbeck), this study analyses the variability and similarity of the hybrids among themselves and with their parents. Random Amplified Polimorfic DNA marker analysis, with 102 primers, were applied to a population composed of 94 hybrids and their parents. Multivariate genetic divergence analysis of the principal components and Tocher grouping were carried out only considering the polymorphic fragments. Genetic distances were calculated by the arithmetic complement of the Jaccard index. Bidimensional dispersion graphs among hybrid and parent distances and of the divergence analysis by principal components were constructed. High genetic similarity among Cravo and Pêra varieties and their hybrids was verified, showing a casual distribution from the hybrids in relation to the parents, but in intermediary positions. The principal component analysis showed little applicability in the study of hybrid genetic divergence. The hybrids and parents were classified in groups based on the genetic similarity, using the Tocher optimization method. |
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Escola Superior de Agricultura "Luiz de Queiroz" |
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2003 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000100017 |
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