Risk factors associated with intramammary colonization with Mollicutes in dairy cattle from Southeast Brazil
ABSTRACT: Bacteria of Mollicutes Class are associated with intramammary infection and decrease in milk production. This study investigated the occurrence of Mollicutes and elucidated their risk factors in dairy herds from Southeast Brazil. For this, milk samples from 387 lactation cows from Minas Gerais, Rio de Janeiro and São Paulo States were subjected to the polymerase chain reaction (PCR) to detect Mollicutes. Species of Mycoplasma were investigated in Mollicutes positive samples by PCR, including Mycoplasma bovis, M. alkalescens, M. bovigenitalium, M. bovirhinis, M. arginini and A. laidlawii. An epidemiological questionnaire was applied to collect data on possible risk factors, which were assessed using Pearson’s Chi-square test followed by odds ratio (P≤0.05). Mollicutes were reported in 21% (4/19) of the herds and 4% (16/387) of the animals, while 1% (5/387) were positive for M. bovis and 3% (11/387) for M. arginini. All samples were negative to the other agents. Herds with more than 150 animals [OR=3.51 (95% CI 1.11-11.08)], manual milking [OR=9.97 (95% CI 2.80-35.49)] and not-milking animals with mastitis last [OR=6.54 (95% CI 1.92-22.29)] were risk factors. The presence of these conditions may favor intramammary infection by Mollicutes in dairy herds from Southeast Brazil. This is the first report of M. bovis in Rio de Janeiro and M. arginini in the studied states.
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Universidade Federal de Santa Maria
2021
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oai:scielo:S0103-847820210008004542021-06-09Risk factors associated with intramammary colonization with Mollicutes in dairy cattle from Southeast BrazilMorais,Ana Carolina Nunes dePires,Danielle RegisCunha,Nathalie Costa daMachado,Leandro dos SantosHelayel,Michel AbdallaMendonça,Juliana França Monteiro deSouza,Guilherme Nunes deBarreto,Maria LuciaNascimento,Elmiro Rosendo do bovine milk Mycoplasma bovis Mycoplasma arginini herd size milking ABSTRACT: Bacteria of Mollicutes Class are associated with intramammary infection and decrease in milk production. This study investigated the occurrence of Mollicutes and elucidated their risk factors in dairy herds from Southeast Brazil. For this, milk samples from 387 lactation cows from Minas Gerais, Rio de Janeiro and São Paulo States were subjected to the polymerase chain reaction (PCR) to detect Mollicutes. Species of Mycoplasma were investigated in Mollicutes positive samples by PCR, including Mycoplasma bovis, M. alkalescens, M. bovigenitalium, M. bovirhinis, M. arginini and A. laidlawii. An epidemiological questionnaire was applied to collect data on possible risk factors, which were assessed using Pearson’s Chi-square test followed by odds ratio (P≤0.05). Mollicutes were reported in 21% (4/19) of the herds and 4% (16/387) of the animals, while 1% (5/387) were positive for M. bovis and 3% (11/387) for M. arginini. All samples were negative to the other agents. Herds with more than 150 animals [OR=3.51 (95% CI 1.11-11.08)], manual milking [OR=9.97 (95% CI 2.80-35.49)] and not-milking animals with mastitis last [OR=6.54 (95% CI 1.92-22.29)] were risk factors. The presence of these conditions may favor intramammary infection by Mollicutes in dairy herds from Southeast Brazil. This is the first report of M. bovis in Rio de Janeiro and M. arginini in the studied states.info:eu-repo/semantics/openAccessUniversidade Federal de Santa MariaCiência Rural v.51 n.8 20212021-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782021000800454en10.1590/0103-8478cr20200694 |
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Morais,Ana Carolina Nunes de Pires,Danielle Regis Cunha,Nathalie Costa da Machado,Leandro dos Santos Helayel,Michel Abdalla Mendonça,Juliana França Monteiro de Souza,Guilherme Nunes de Barreto,Maria Lucia Nascimento,Elmiro Rosendo do |
| spellingShingle |
Morais,Ana Carolina Nunes de Pires,Danielle Regis Cunha,Nathalie Costa da Machado,Leandro dos Santos Helayel,Michel Abdalla Mendonça,Juliana França Monteiro de Souza,Guilherme Nunes de Barreto,Maria Lucia Nascimento,Elmiro Rosendo do Risk factors associated with intramammary colonization with Mollicutes in dairy cattle from Southeast Brazil |
| author_facet |
Morais,Ana Carolina Nunes de Pires,Danielle Regis Cunha,Nathalie Costa da Machado,Leandro dos Santos Helayel,Michel Abdalla Mendonça,Juliana França Monteiro de Souza,Guilherme Nunes de Barreto,Maria Lucia Nascimento,Elmiro Rosendo do |
| author_sort |
Morais,Ana Carolina Nunes de |
| title |
Risk factors associated with intramammary colonization with Mollicutes in dairy cattle from Southeast Brazil |
| title_short |
Risk factors associated with intramammary colonization with Mollicutes in dairy cattle from Southeast Brazil |
| title_full |
Risk factors associated with intramammary colonization with Mollicutes in dairy cattle from Southeast Brazil |
| title_fullStr |
Risk factors associated with intramammary colonization with Mollicutes in dairy cattle from Southeast Brazil |
| title_full_unstemmed |
Risk factors associated with intramammary colonization with Mollicutes in dairy cattle from Southeast Brazil |
| title_sort |
risk factors associated with intramammary colonization with mollicutes in dairy cattle from southeast brazil |
| description |
ABSTRACT: Bacteria of Mollicutes Class are associated with intramammary infection and decrease in milk production. This study investigated the occurrence of Mollicutes and elucidated their risk factors in dairy herds from Southeast Brazil. For this, milk samples from 387 lactation cows from Minas Gerais, Rio de Janeiro and São Paulo States were subjected to the polymerase chain reaction (PCR) to detect Mollicutes. Species of Mycoplasma were investigated in Mollicutes positive samples by PCR, including Mycoplasma bovis, M. alkalescens, M. bovigenitalium, M. bovirhinis, M. arginini and A. laidlawii. An epidemiological questionnaire was applied to collect data on possible risk factors, which were assessed using Pearson’s Chi-square test followed by odds ratio (P≤0.05). Mollicutes were reported in 21% (4/19) of the herds and 4% (16/387) of the animals, while 1% (5/387) were positive for M. bovis and 3% (11/387) for M. arginini. All samples were negative to the other agents. Herds with more than 150 animals [OR=3.51 (95% CI 1.11-11.08)], manual milking [OR=9.97 (95% CI 2.80-35.49)] and not-milking animals with mastitis last [OR=6.54 (95% CI 1.92-22.29)] were risk factors. The presence of these conditions may favor intramammary infection by Mollicutes in dairy herds from Southeast Brazil. This is the first report of M. bovis in Rio de Janeiro and M. arginini in the studied states. |
| publisher |
Universidade Federal de Santa Maria |
| publishDate |
2021 |
| url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782021000800454 |
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