Fusarium oxysporum dispersion by larvae of Cyclocephala modesta, Dyscinetus gagates and Diloboderus abderus in Brazil

ABSTRACT: The scientific and economic importance of soybean root rot justifies the study of this disease, especially the role of insects as dispersers in the spread of root pathogens. The objective was to identify and evaluate qualitatively and quantitatively the role of Cyclocephala modesta, Dyscinetus gagatesand Diloboderus abderus(Coleoptera: Melolonthidae) in the dispersion of root pathogens in cultivated fields. The fungi were identified to the species level with DNA extraction and sequencing of these organisms within Melolonthidae larvae. Larvae were dissected and separated into the parts: mouth parts, prothorax, cuticle and digestive tract (alimentary canal). The Internal Transcribed Spacer region of the rRNA was amplified and compared to sequences deposited in GenBank. This is the first record of dispersion Fusarium oxysporumby Cyclocephala modesta, Dyscinetus gagatesand Diloboderus abderus (Coleoptera: Melolonthidae) under soybean in Brazil.

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Main Authors: Salgado-Neto,Geraldo, Vaz,Marcos André Braz, Guedes,Jerson Vanderlei Carús, Muniz,Marlove Fátima Brião, Blume,Elena
Format: Digital revista
Language:English
Published: Universidade Federal de Santa Maria 2016
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000600943
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spelling oai:scielo:S0103-847820160006009432016-05-03Fusarium oxysporum dispersion by larvae of Cyclocephala modesta, Dyscinetus gagates and Diloboderus abderus in BrazilSalgado-Neto,GeraldoVaz,Marcos André BrazGuedes,Jerson Vanderlei CarúsMuniz,Marlove Fátima BriãoBlume,Elena Biological control dissemination Fusarium oxysporum interactions Melolonthidae white grubs ABSTRACT: The scientific and economic importance of soybean root rot justifies the study of this disease, especially the role of insects as dispersers in the spread of root pathogens. The objective was to identify and evaluate qualitatively and quantitatively the role of Cyclocephala modesta, Dyscinetus gagatesand Diloboderus abderus(Coleoptera: Melolonthidae) in the dispersion of root pathogens in cultivated fields. The fungi were identified to the species level with DNA extraction and sequencing of these organisms within Melolonthidae larvae. Larvae were dissected and separated into the parts: mouth parts, prothorax, cuticle and digestive tract (alimentary canal). The Internal Transcribed Spacer region of the rRNA was amplified and compared to sequences deposited in GenBank. This is the first record of dispersion Fusarium oxysporumby Cyclocephala modesta, Dyscinetus gagatesand Diloboderus abderus (Coleoptera: Melolonthidae) under soybean in Brazil.info:eu-repo/semantics/openAccessUniversidade Federal de Santa MariaCiência Rural v.46 n.6 20162016-06-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000600943en10.1590/0103-8478cr20150471
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Salgado-Neto,Geraldo
Vaz,Marcos André Braz
Guedes,Jerson Vanderlei Carús
Muniz,Marlove Fátima Brião
Blume,Elena
spellingShingle Salgado-Neto,Geraldo
Vaz,Marcos André Braz
Guedes,Jerson Vanderlei Carús
Muniz,Marlove Fátima Brião
Blume,Elena
Fusarium oxysporum dispersion by larvae of Cyclocephala modesta, Dyscinetus gagates and Diloboderus abderus in Brazil
author_facet Salgado-Neto,Geraldo
Vaz,Marcos André Braz
Guedes,Jerson Vanderlei Carús
Muniz,Marlove Fátima Brião
Blume,Elena
author_sort Salgado-Neto,Geraldo
title Fusarium oxysporum dispersion by larvae of Cyclocephala modesta, Dyscinetus gagates and Diloboderus abderus in Brazil
title_short Fusarium oxysporum dispersion by larvae of Cyclocephala modesta, Dyscinetus gagates and Diloboderus abderus in Brazil
title_full Fusarium oxysporum dispersion by larvae of Cyclocephala modesta, Dyscinetus gagates and Diloboderus abderus in Brazil
title_fullStr Fusarium oxysporum dispersion by larvae of Cyclocephala modesta, Dyscinetus gagates and Diloboderus abderus in Brazil
title_full_unstemmed Fusarium oxysporum dispersion by larvae of Cyclocephala modesta, Dyscinetus gagates and Diloboderus abderus in Brazil
title_sort fusarium oxysporum dispersion by larvae of cyclocephala modesta, dyscinetus gagates and diloboderus abderus in brazil
description ABSTRACT: The scientific and economic importance of soybean root rot justifies the study of this disease, especially the role of insects as dispersers in the spread of root pathogens. The objective was to identify and evaluate qualitatively and quantitatively the role of Cyclocephala modesta, Dyscinetus gagatesand Diloboderus abderus(Coleoptera: Melolonthidae) in the dispersion of root pathogens in cultivated fields. The fungi were identified to the species level with DNA extraction and sequencing of these organisms within Melolonthidae larvae. Larvae were dissected and separated into the parts: mouth parts, prothorax, cuticle and digestive tract (alimentary canal). The Internal Transcribed Spacer region of the rRNA was amplified and compared to sequences deposited in GenBank. This is the first record of dispersion Fusarium oxysporumby Cyclocephala modesta, Dyscinetus gagatesand Diloboderus abderus (Coleoptera: Melolonthidae) under soybean in Brazil.
publisher Universidade Federal de Santa Maria
publishDate 2016
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000600943
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