Fusarium oxysporum dispersion by larvae of Cyclocephala modesta, Dyscinetus gagates and Diloboderus abderus in Brazil
ABSTRACT: The scientific and economic importance of soybean root rot justifies the study of this disease, especially the role of insects as dispersers in the spread of root pathogens. The objective was to identify and evaluate qualitatively and quantitatively the role of Cyclocephala modesta, Dyscinetus gagatesand Diloboderus abderus(Coleoptera: Melolonthidae) in the dispersion of root pathogens in cultivated fields. The fungi were identified to the species level with DNA extraction and sequencing of these organisms within Melolonthidae larvae. Larvae were dissected and separated into the parts: mouth parts, prothorax, cuticle and digestive tract (alimentary canal). The Internal Transcribed Spacer region of the rRNA was amplified and compared to sequences deposited in GenBank. This is the first record of dispersion Fusarium oxysporumby Cyclocephala modesta, Dyscinetus gagatesand Diloboderus abderus (Coleoptera: Melolonthidae) under soybean in Brazil.
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Universidade Federal de Santa Maria
2016
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oai:scielo:S0103-847820160006009432016-05-03Fusarium oxysporum dispersion by larvae of Cyclocephala modesta, Dyscinetus gagates and Diloboderus abderus in BrazilSalgado-Neto,GeraldoVaz,Marcos André BrazGuedes,Jerson Vanderlei CarúsMuniz,Marlove Fátima BriãoBlume,Elena Biological control dissemination Fusarium oxysporum interactions Melolonthidae white grubs ABSTRACT: The scientific and economic importance of soybean root rot justifies the study of this disease, especially the role of insects as dispersers in the spread of root pathogens. The objective was to identify and evaluate qualitatively and quantitatively the role of Cyclocephala modesta, Dyscinetus gagatesand Diloboderus abderus(Coleoptera: Melolonthidae) in the dispersion of root pathogens in cultivated fields. The fungi were identified to the species level with DNA extraction and sequencing of these organisms within Melolonthidae larvae. Larvae were dissected and separated into the parts: mouth parts, prothorax, cuticle and digestive tract (alimentary canal). The Internal Transcribed Spacer region of the rRNA was amplified and compared to sequences deposited in GenBank. This is the first record of dispersion Fusarium oxysporumby Cyclocephala modesta, Dyscinetus gagatesand Diloboderus abderus (Coleoptera: Melolonthidae) under soybean in Brazil.info:eu-repo/semantics/openAccessUniversidade Federal de Santa MariaCiência Rural v.46 n.6 20162016-06-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000600943en10.1590/0103-8478cr20150471 |
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Salgado-Neto,Geraldo Vaz,Marcos André Braz Guedes,Jerson Vanderlei Carús Muniz,Marlove Fátima Brião Blume,Elena |
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Salgado-Neto,Geraldo Vaz,Marcos André Braz Guedes,Jerson Vanderlei Carús Muniz,Marlove Fátima Brião Blume,Elena Fusarium oxysporum dispersion by larvae of Cyclocephala modesta, Dyscinetus gagates and Diloboderus abderus in Brazil |
author_facet |
Salgado-Neto,Geraldo Vaz,Marcos André Braz Guedes,Jerson Vanderlei Carús Muniz,Marlove Fátima Brião Blume,Elena |
author_sort |
Salgado-Neto,Geraldo |
title |
Fusarium oxysporum dispersion by larvae of Cyclocephala modesta, Dyscinetus gagates and Diloboderus abderus in Brazil |
title_short |
Fusarium oxysporum dispersion by larvae of Cyclocephala modesta, Dyscinetus gagates and Diloboderus abderus in Brazil |
title_full |
Fusarium oxysporum dispersion by larvae of Cyclocephala modesta, Dyscinetus gagates and Diloboderus abderus in Brazil |
title_fullStr |
Fusarium oxysporum dispersion by larvae of Cyclocephala modesta, Dyscinetus gagates and Diloboderus abderus in Brazil |
title_full_unstemmed |
Fusarium oxysporum dispersion by larvae of Cyclocephala modesta, Dyscinetus gagates and Diloboderus abderus in Brazil |
title_sort |
fusarium oxysporum dispersion by larvae of cyclocephala modesta, dyscinetus gagates and diloboderus abderus in brazil |
description |
ABSTRACT: The scientific and economic importance of soybean root rot justifies the study of this disease, especially the role of insects as dispersers in the spread of root pathogens. The objective was to identify and evaluate qualitatively and quantitatively the role of Cyclocephala modesta, Dyscinetus gagatesand Diloboderus abderus(Coleoptera: Melolonthidae) in the dispersion of root pathogens in cultivated fields. The fungi were identified to the species level with DNA extraction and sequencing of these organisms within Melolonthidae larvae. Larvae were dissected and separated into the parts: mouth parts, prothorax, cuticle and digestive tract (alimentary canal). The Internal Transcribed Spacer region of the rRNA was amplified and compared to sequences deposited in GenBank. This is the first record of dispersion Fusarium oxysporumby Cyclocephala modesta, Dyscinetus gagatesand Diloboderus abderus (Coleoptera: Melolonthidae) under soybean in Brazil. |
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Universidade Federal de Santa Maria |
publishDate |
2016 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000600943 |
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