Prevention of Clostridium difficile infection in hamsters using a non-toxigenic strain

Prevention of Clostridium difficile infection in hamsters using a non-toxigenic strain

ABSTRACT: The present study aimed to evaluate five non-toxigenic strains of Clostridium difficile (NTCD) in vitro and to select one strain to prevent C. difficile (CDI) infection in hamsters ( Mesocricetus auratus ). The NTCD strains were evaluated for spore production in vitro, antimicrobial susceptibility and presence of antimicrobial resistance genes. Approximately 107 spores of the selected strain (Z31) were administered by esophageal gavage in hamsters pretreated with 30mg kg-1 of clindamycin. The challenge with a toxigenic strain of C. difficile was conducted at 36 and 72h, and the animals were observed for 28 days. The NTCD strain of C. difficile (Z31) was able to prevent CDI in all animals that received it.

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Main Authors: Oliveira Júnior,Carlos Augusto de, Silva,Rodrigo Otávio Silveira, Diniz,Amanda Nádia, Pires,Prhiscylla Sadanã, Lobato,Francisco Carlos Faria, Assis,Ronie Antunes de
Format: Digital revista
Language:English
Published: Universidade Federal de Santa Maria 2016
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000500853
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spelling oai:scielo:S0103-847820160005008532016-10-20Prevention of Clostridium difficile infection in hamsters using a non-toxigenic strainOliveira Júnior,Carlos Augusto deSilva,Rodrigo Otávio SilveiraDiniz,Amanda NádiaPires,Prhiscylla SadanãLobato,Francisco Carlos FariaAssis,Ronie Antunes de diarrhea Clostridium difficile- associated diseases colitis ABSTRACT: The present study aimed to evaluate five non-toxigenic strains of Clostridium difficile (NTCD) in vitro and to select one strain to prevent C. difficile (CDI) infection in hamsters ( Mesocricetus auratus ). The NTCD strains were evaluated for spore production in vitro, antimicrobial susceptibility and presence of antimicrobial resistance genes. Approximately 107 spores of the selected strain (Z31) were administered by esophageal gavage in hamsters pretreated with 30mg kg-1 of clindamycin. The challenge with a toxigenic strain of C. difficile was conducted at 36 and 72h, and the animals were observed for 28 days. The NTCD strain of C. difficile (Z31) was able to prevent CDI in all animals that received it.info:eu-repo/semantics/openAccessUniversidade Federal de Santa MariaCiência Rural v.46 n.5 20162016-05-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000500853en10.1590/0103-8478cr20150454
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country Brasil
countrycode BR
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databasecode rev-scielo-br
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libraryname SciELO
language English
format Digital
author Oliveira Júnior,Carlos Augusto de
Silva,Rodrigo Otávio Silveira
Diniz,Amanda Nádia
Pires,Prhiscylla Sadanã
Lobato,Francisco Carlos Faria
Assis,Ronie Antunes de
spellingShingle Oliveira Júnior,Carlos Augusto de
Silva,Rodrigo Otávio Silveira
Diniz,Amanda Nádia
Pires,Prhiscylla Sadanã
Lobato,Francisco Carlos Faria
Assis,Ronie Antunes de
Prevention of Clostridium difficile infection in hamsters using a non-toxigenic strain
author_facet Oliveira Júnior,Carlos Augusto de
Silva,Rodrigo Otávio Silveira
Diniz,Amanda Nádia
Pires,Prhiscylla Sadanã
Lobato,Francisco Carlos Faria
Assis,Ronie Antunes de
author_sort Oliveira Júnior,Carlos Augusto de
title Prevention of Clostridium difficile infection in hamsters using a non-toxigenic strain
title_short Prevention of Clostridium difficile infection in hamsters using a non-toxigenic strain
title_full Prevention of Clostridium difficile infection in hamsters using a non-toxigenic strain
title_fullStr Prevention of Clostridium difficile infection in hamsters using a non-toxigenic strain
title_full_unstemmed Prevention of Clostridium difficile infection in hamsters using a non-toxigenic strain
title_sort prevention of clostridium difficile infection in hamsters using a non-toxigenic strain
description ABSTRACT: The present study aimed to evaluate five non-toxigenic strains of Clostridium difficile (NTCD) in vitro and to select one strain to prevent C. difficile (CDI) infection in hamsters ( Mesocricetus auratus ). The NTCD strains were evaluated for spore production in vitro, antimicrobial susceptibility and presence of antimicrobial resistance genes. Approximately 107 spores of the selected strain (Z31) were administered by esophageal gavage in hamsters pretreated with 30mg kg-1 of clindamycin. The challenge with a toxigenic strain of C. difficile was conducted at 36 and 72h, and the animals were observed for 28 days. The NTCD strain of C. difficile (Z31) was able to prevent CDI in all animals that received it.
publisher Universidade Federal de Santa Maria
publishDate 2016
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000500853
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