Molecular characterization of Listeria monocytogenes isolated from animal products in a city of Northern Brazil

Molecular characterization of Listeria monocytogenes isolated from animal products in a city of Northern Brazil

Listeria monocytogenes, a foodborne pathogen causes listeriosis, a fatal disease in about 30% of cases that affects mainly immunocompromised persons. The aim of this research was to characterize L. monocytogenes pulsed-field gel electrophoresis (PFGE) types isolated from meat products collected at public markets in Araguaina city, TO. Sixty samples of raw ground beef and frescal sausage were analyzed during the second half of 2008. Five out of 30 samples (16.7%) of raw ground beef tested positive for L. monocytogenes, three of which were classified as serotype 1/2b and two as serotype 4b. Among the 30 samples of sausage collected, two strains of L. monocytogenes were isolated (6.7%), one of them belonging to serotype 1/2a and the other belonging to serotype 1/2b. The restriction enzymes used were ApaI and SmaI. Similarities among the strains were determined by Dice coefficient. The macro restriction profile obtained by using SmaI enzyme allowed the distribution of seven strains in two clusters, two pulsotypes and two subtypes. The result indicates that L. monocytogenes isolates, belonging to serotype 4b, 1/2a and 1/2b, are strongly correlated within the same serotype group, and in some cases among different serotypes, suggesting that they have a common source.

Saved in:
Bibliographic Details
Main Authors: Monteiro,Lilyan Rosmery Luizaga de, Mesquita,Albenones José de, André,Maria Cláudia Dantas Porfirio Borges, Cardoso,Juliana Lamaro
Format: Digital revista
Language:English
Published: Universidade Federal de Santa Maria 2013
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000800016
Tags: Add Tag
No Tags, Be the first to tag this record!
id oai:scielo:S0103-84782013000800016
record_format ojs
spelling oai:scielo:S0103-847820130008000162013-07-30Molecular characterization of Listeria monocytogenes isolated from animal products in a city of Northern BrazilMonteiro,Lilyan Rosmery Luizaga deMesquita,Albenones José deAndré,Maria Cláudia Dantas Porfirio BorgesCardoso,Juliana Lamaro pulsed field gel electrophoresis ground beef frescal sausage L. monocytogenes Listeria monocytogenes, a foodborne pathogen causes listeriosis, a fatal disease in about 30% of cases that affects mainly immunocompromised persons. The aim of this research was to characterize L. monocytogenes pulsed-field gel electrophoresis (PFGE) types isolated from meat products collected at public markets in Araguaina city, TO. Sixty samples of raw ground beef and frescal sausage were analyzed during the second half of 2008. Five out of 30 samples (16.7%) of raw ground beef tested positive for L. monocytogenes, three of which were classified as serotype 1/2b and two as serotype 4b. Among the 30 samples of sausage collected, two strains of L. monocytogenes were isolated (6.7%), one of them belonging to serotype 1/2a and the other belonging to serotype 1/2b. The restriction enzymes used were ApaI and SmaI. Similarities among the strains were determined by Dice coefficient. The macro restriction profile obtained by using SmaI enzyme allowed the distribution of seven strains in two clusters, two pulsotypes and two subtypes. The result indicates that L. monocytogenes isolates, belonging to serotype 4b, 1/2a and 1/2b, are strongly correlated within the same serotype group, and in some cases among different serotypes, suggesting that they have a common source.info:eu-repo/semantics/openAccessUniversidade Federal de Santa MariaCiência Rural v.43 n.8 20132013-08-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000800016en10.1590/S0103-84782013000800016
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Monteiro,Lilyan Rosmery Luizaga de
Mesquita,Albenones José de
André,Maria Cláudia Dantas Porfirio Borges
Cardoso,Juliana Lamaro
spellingShingle Monteiro,Lilyan Rosmery Luizaga de
Mesquita,Albenones José de
André,Maria Cláudia Dantas Porfirio Borges
Cardoso,Juliana Lamaro
Molecular characterization of Listeria monocytogenes isolated from animal products in a city of Northern Brazil
author_facet Monteiro,Lilyan Rosmery Luizaga de
Mesquita,Albenones José de
André,Maria Cláudia Dantas Porfirio Borges
Cardoso,Juliana Lamaro
author_sort Monteiro,Lilyan Rosmery Luizaga de
title Molecular characterization of Listeria monocytogenes isolated from animal products in a city of Northern Brazil
title_short Molecular characterization of Listeria monocytogenes isolated from animal products in a city of Northern Brazil
title_full Molecular characterization of Listeria monocytogenes isolated from animal products in a city of Northern Brazil
title_fullStr Molecular characterization of Listeria monocytogenes isolated from animal products in a city of Northern Brazil
title_full_unstemmed Molecular characterization of Listeria monocytogenes isolated from animal products in a city of Northern Brazil
title_sort molecular characterization of listeria monocytogenes isolated from animal products in a city of northern brazil
description Listeria monocytogenes, a foodborne pathogen causes listeriosis, a fatal disease in about 30% of cases that affects mainly immunocompromised persons. The aim of this research was to characterize L. monocytogenes pulsed-field gel electrophoresis (PFGE) types isolated from meat products collected at public markets in Araguaina city, TO. Sixty samples of raw ground beef and frescal sausage were analyzed during the second half of 2008. Five out of 30 samples (16.7%) of raw ground beef tested positive for L. monocytogenes, three of which were classified as serotype 1/2b and two as serotype 4b. Among the 30 samples of sausage collected, two strains of L. monocytogenes were isolated (6.7%), one of them belonging to serotype 1/2a and the other belonging to serotype 1/2b. The restriction enzymes used were ApaI and SmaI. Similarities among the strains were determined by Dice coefficient. The macro restriction profile obtained by using SmaI enzyme allowed the distribution of seven strains in two clusters, two pulsotypes and two subtypes. The result indicates that L. monocytogenes isolates, belonging to serotype 4b, 1/2a and 1/2b, are strongly correlated within the same serotype group, and in some cases among different serotypes, suggesting that they have a common source.
publisher Universidade Federal de Santa Maria
publishDate 2013
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000800016
work_keys_str_mv AT monteirolilyanrosmeryluizagade molecularcharacterizationoflisteriamonocytogenesisolatedfromanimalproductsinacityofnorthernbrazil
AT mesquitaalbenonesjosede molecularcharacterizationoflisteriamonocytogenesisolatedfromanimalproductsinacityofnorthernbrazil
AT andremariaclaudiadantasporfirioborges molecularcharacterizationoflisteriamonocytogenesisolatedfromanimalproductsinacityofnorthernbrazil
AT cardosojulianalamaro molecularcharacterizationoflisteriamonocytogenesisolatedfromanimalproductsinacityofnorthernbrazil
_version_ 1756406180124557312

Similar Items