Molecular typing of Clostridium perfringens isolated from swine in slaughterhouses from São Paulo State, Brazil

Molecular typing of Clostridium perfringens isolated from swine in slaughterhouses from São Paulo State, Brazil

Clostridium perfringens is an anaerobic Gram-positive bacterium known as common pathogen for humans, for domestic and wildlife animals. Although infections caused by C. perfringens type C and A in swine are well studied, just a few reports describe the genetic relationship among strains in the epidemiological chain of swine clostridioses, as well as the presence of the microorganism in the slaughterhouses. The aim of the present study was to isolate C. perfringens from feces and carcasses from swine slaughterhouses, characterize the strains in relation to the presence of enterotoxin, alpha, beta, epsilon, iota and beta-2 toxins genes, using polymerase chain reaction (PCR) and comparing strains by means of Pulsed field gel electrophoresis (PFGE). Clostridium perfringens isolation frequencies in carcasses and finishing pig intestines were of 58.8% in both types of samples. According to the polymerase chain reaction assay, only alfa toxin was detected, being all isolates also negative to enterotoxin and beta2 toxin. Through PFGE technique, the strains were characterized in 35 pulsotypes. In only one pulsotype, the isolate from carcass sample was grouped with fecal isolate of the same animal, suggesting that the risk of cross-contamination was low. Despite the high prevalence of C. perfringens in swine carcasses from the slaughterhouses assessed, the risk of food poisoning to Brazilian pork consumers is low, since all strains were negative to cpe-gene, codifying enterotoxin.

Saved in:
Bibliographic Details
Main Authors: Ferreira,Thais Sebastiana Porfida, Moreno,Andrea Micke, Almeida,Renata Rodrigues de, Gomes,Cleise Ribeiro, Gobbi,Debora Dirani Sena de, Filsner,Pedro Henrique Nogueira de Lima, Moreno,Marina
Format: Digital revista
Language:English
Published: Universidade Federal de Santa Maria 2012
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000800020
Tags: Add Tag
No Tags, Be the first to tag this record!
id oai:scielo:S0103-84782012000800020
record_format ojs
spelling oai:scielo:S0103-847820120008000202012-09-04Molecular typing of Clostridium perfringens isolated from swine in slaughterhouses from São Paulo State, BrazilFerreira,Thais Sebastiana PorfidaMoreno,Andrea MickeAlmeida,Renata Rodrigues deGomes,Cleise RibeiroGobbi,Debora Dirani Sena deFilsner,Pedro Henrique Nogueira de LimaMoreno,Marina Clostridium perfringens swine slaughterhouse PFGE carcass toxins Clostridium perfringens is an anaerobic Gram-positive bacterium known as common pathogen for humans, for domestic and wildlife animals. Although infections caused by C. perfringens type C and A in swine are well studied, just a few reports describe the genetic relationship among strains in the epidemiological chain of swine clostridioses, as well as the presence of the microorganism in the slaughterhouses. The aim of the present study was to isolate C. perfringens from feces and carcasses from swine slaughterhouses, characterize the strains in relation to the presence of enterotoxin, alpha, beta, epsilon, iota and beta-2 toxins genes, using polymerase chain reaction (PCR) and comparing strains by means of Pulsed field gel electrophoresis (PFGE). Clostridium perfringens isolation frequencies in carcasses and finishing pig intestines were of 58.8% in both types of samples. According to the polymerase chain reaction assay, only alfa toxin was detected, being all isolates also negative to enterotoxin and beta2 toxin. Through PFGE technique, the strains were characterized in 35 pulsotypes. In only one pulsotype, the isolate from carcass sample was grouped with fecal isolate of the same animal, suggesting that the risk of cross-contamination was low. Despite the high prevalence of C. perfringens in swine carcasses from the slaughterhouses assessed, the risk of food poisoning to Brazilian pork consumers is low, since all strains were negative to cpe-gene, codifying enterotoxin.info:eu-repo/semantics/openAccessUniversidade Federal de Santa MariaCiência Rural v.42 n.8 20122012-08-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000800020en10.1590/S0103-84782012000800020
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Ferreira,Thais Sebastiana Porfida
Moreno,Andrea Micke
Almeida,Renata Rodrigues de
Gomes,Cleise Ribeiro
Gobbi,Debora Dirani Sena de
Filsner,Pedro Henrique Nogueira de Lima
Moreno,Marina
spellingShingle Ferreira,Thais Sebastiana Porfida
Moreno,Andrea Micke
Almeida,Renata Rodrigues de
Gomes,Cleise Ribeiro
Gobbi,Debora Dirani Sena de
Filsner,Pedro Henrique Nogueira de Lima
Moreno,Marina
Molecular typing of Clostridium perfringens isolated from swine in slaughterhouses from São Paulo State, Brazil
author_facet Ferreira,Thais Sebastiana Porfida
Moreno,Andrea Micke
Almeida,Renata Rodrigues de
Gomes,Cleise Ribeiro
Gobbi,Debora Dirani Sena de
Filsner,Pedro Henrique Nogueira de Lima
Moreno,Marina
author_sort Ferreira,Thais Sebastiana Porfida
title Molecular typing of Clostridium perfringens isolated from swine in slaughterhouses from São Paulo State, Brazil
title_short Molecular typing of Clostridium perfringens isolated from swine in slaughterhouses from São Paulo State, Brazil
title_full Molecular typing of Clostridium perfringens isolated from swine in slaughterhouses from São Paulo State, Brazil
title_fullStr Molecular typing of Clostridium perfringens isolated from swine in slaughterhouses from São Paulo State, Brazil
title_full_unstemmed Molecular typing of Clostridium perfringens isolated from swine in slaughterhouses from São Paulo State, Brazil
title_sort molecular typing of clostridium perfringens isolated from swine in slaughterhouses from são paulo state, brazil
description Clostridium perfringens is an anaerobic Gram-positive bacterium known as common pathogen for humans, for domestic and wildlife animals. Although infections caused by C. perfringens type C and A in swine are well studied, just a few reports describe the genetic relationship among strains in the epidemiological chain of swine clostridioses, as well as the presence of the microorganism in the slaughterhouses. The aim of the present study was to isolate C. perfringens from feces and carcasses from swine slaughterhouses, characterize the strains in relation to the presence of enterotoxin, alpha, beta, epsilon, iota and beta-2 toxins genes, using polymerase chain reaction (PCR) and comparing strains by means of Pulsed field gel electrophoresis (PFGE). Clostridium perfringens isolation frequencies in carcasses and finishing pig intestines were of 58.8% in both types of samples. According to the polymerase chain reaction assay, only alfa toxin was detected, being all isolates also negative to enterotoxin and beta2 toxin. Through PFGE technique, the strains were characterized in 35 pulsotypes. In only one pulsotype, the isolate from carcass sample was grouped with fecal isolate of the same animal, suggesting that the risk of cross-contamination was low. Despite the high prevalence of C. perfringens in swine carcasses from the slaughterhouses assessed, the risk of food poisoning to Brazilian pork consumers is low, since all strains were negative to cpe-gene, codifying enterotoxin.
publisher Universidade Federal de Santa Maria
publishDate 2012
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000800020
work_keys_str_mv AT ferreirathaissebastianaporfida moleculartypingofclostridiumperfringensisolatedfromswineinslaughterhousesfromsaopaulostatebrazil
AT morenoandreamicke moleculartypingofclostridiumperfringensisolatedfromswineinslaughterhousesfromsaopaulostatebrazil
AT almeidarenatarodriguesde moleculartypingofclostridiumperfringensisolatedfromswineinslaughterhousesfromsaopaulostatebrazil
AT gomescleiseribeiro moleculartypingofclostridiumperfringensisolatedfromswineinslaughterhousesfromsaopaulostatebrazil
AT gobbideboradiranisenade moleculartypingofclostridiumperfringensisolatedfromswineinslaughterhousesfromsaopaulostatebrazil
AT filsnerpedrohenriquenogueiradelima moleculartypingofclostridiumperfringensisolatedfromswineinslaughterhousesfromsaopaulostatebrazil
AT morenomarina moleculartypingofclostridiumperfringensisolatedfromswineinslaughterhousesfromsaopaulostatebrazil
_version_ 1756406106180026368

Similar Items