Callus induction and plant regeneration of tomato (Lycopersicon esculentum cv. IPA 5) via anther culture
Different growth regulators combinations were tested on the production of anther callus in tomato cultivar IPA 5. Calli were induced on media supplemented with 1.0mgL-1 gibberellic acid (GA3), 0.05mgL-1 alpha-naphthaleneacetic acid (NAA) plus 0.1mgL-1 6-benzylaminopurine (BAP), or with 1.0mgL-1 BAP plus 1.0mgL-1 NAA. The medium containing 1.0mgL-1 BAP and 1.0mgL-1 NAA produced the highest calli frequency, and promoted plant regeneration by indirect organogenesis, when calli were transferred to 0.01mgL-1 BAP and 0.001mgL-1 NAA. Plants regenerated presented tetraploid cells and rare diploid cells. These tetraploid plants could be used as source for further obtainment of trisomic lines, for the purpose of genic localization studies and protein compounds analysis.
| Main Authors: | , , , |
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| Format: | Digital revista |
| Language: | English |
| Published: |
Universidade Federal de Santa Maria
1999
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| Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781999000400008 |
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