Simultaneous Determination of Four Antibiotics in Raw Milk by UPLC-MS/MS Using Protein Precipitation as Sample Preparation: Development, Validation, and Application in Real Samples
In this study, a rapid and simple analytical method was proposed, based on protein precipitation as sample preparation for simultaneous determination of tetracycline, oxytetracycline, penicillin G and ceftiofur in raw milk by ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). The method was applied to raw milk samples from dairy cows medicated with tetracycline for subclinical mastitis. Samples were collected from the adjacent teat to the teat treated with tetracycline of eight different cows at 24, 48, 72 and 96 h after treatment. The limits of quantification of the proposed method ranged between 1 and 5 ng g-1 and limits of detection ranged between 0.1 and 0.5 ng g-1. The recoveries ranged from 61 to 111% and the linear range was 1 to 2064 ng g-1 for tetracycline and oxytetracycline, and 5 to 2064 ng g-1 for penicillin G and ceftiofur. Approximately 75 and 63% of the treated animals revealed more tetracycline than legally recommended at 72 and 96 h since last treatment, respectively.
Main Authors: | Magon,Thiago, Silveira,Roberta da, Galuch,Marília B., Fagan,Eder Paulo, Feitoza,Ana Flavia D., Palombini,Sylvio V., Santos,Oscar O., Visentainer,Jesui V. |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Sociedade Brasileira de Química
2018
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532018001102441 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Evaluation of an alternative spectroscopic approach for aflatoxin analysis: Comparative analysis of food and feed samples with UPLC–MS/MS
by: Campbell, Katrina, et al. -
Multi-residue method of pesticides by UPLC-MS/MS in bivalve mollusks samples as a tool for food quality and safety.
by: CASTRO, I. M. de, et al.
Published: (2023-07-12) -
Development of a rapid method for the determination of antibiotic residues in honey using UPLC-ESI-MS/MS
by: KIVRAK,İbrahim, et al.
Published: (2016) -
UPLC-MS/MS and Dushman reaction based spectrophotometric method for determination of Ceftazidime, an antibiotic, in medicinal formulation
by: SIDDIQUI,Masoom Raza, et al.
Published: (2021) -
Pesticide Residues Method Validation by UPLC-MS/MS for Accreditation Purposes
by: Pizzutti,Ionara R., et al.
Published: (2016)