Development and validation of a capillary electrophoresis method for the determination of sulfate in indinavir sulfate raw material
In this work, a simple and fast capillary electrophoresis method using indirect UV detection (230 nm) for the quantitative determination of sulfate in the indinavir sulfate raw material was described. A running electrolyte consisting of 10 mmol L-1 ammonium molybdate containing 0.15 mmol L-1 cetyltrimethylammonium bromide (pH 7.5) was used. Samples were injected hydrodynamically by applying 5000 Pa pressure during 3 s. The response was linear from 10.1 µg mL-1 to 79.8 mg µL-1. Limits of detection and quantification were 0.34 and 1.13 mg µL-1, respectively. The intra-day precision expressed as relative standard deviation was 2.8% for 10 independent sample preparations. Recoveries varying from 96.9 to 102.4% at three concentration levels were obtained. The methodology was successfully applied for real samples. The concentration of sulfate in indinavir sulfate raw material varied from 13.3 to 13.5% (m/m calculated as anhydrous base).
| Auteurs principaux: | , , |
|---|---|
| Format: | Digital revista |
| Langue: | English |
| Publié: |
Sociedade Brasileira de Química
2006
|
| Accès en ligne: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532006000200006 |
| Tags: |
Ajouter un tag
Pas de tags, Soyez le premier à ajouter un tag!
|