Experimental model of ultrasound thermotherapy in rats inoculated with Walker-236 tumor
PURPOSE: To develop a model to evaluate the effects of focal pulsed ultrasound (US) waves as a source of heat for treatment of murine subcutaneous implanted Walker tumor. METHODS: An experimental, controlled, comparative study was conducted. Twenty male Wistar rats (160-300 g) randomized in 2 equal groups (G-1: Control and G-2: Hyperthermia) were inoculated with Walker-256 carcinosarcoma tumor. After 5 days G-2 rats were submitted to 45ºC hyperthermia. Heat was delivered directly to the tumor by an ultrasound (US) equipment (3 MHz frequency, 1,5W/cm³). Tumor temperature reached 45º C in 3 minutes and was maintained at this level for 5 minutes. Tumor volume was measured on days 5, 8, 11, 14 e 17 post inoculation in both groups. Unpaired t-test was used for comparison. P<0.05 was considered significant. RESULTS: Tumor volume was significantly greater in day 5 and decreased in days 11, 14 and 17 in treated rats. Rats treated with hyperthermia survived longer than control animals. On the 29th day following tumor inoculation, 40% of control rats and 77.78% of hyperthermia-treated rats remained alive. CONCLUSION: The proposed model is quite simple and may be used in less sophisticated laboratory settings for studying the effects of focal hyperthermia in the treatment of malignant implanted tumours or in survival studies.
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2011
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oai:scielo:S0102-865020110007000112011-09-23Experimental model of ultrasound thermotherapy in rats inoculated with Walker-236 tumorMorano,José Antonio Carlos Otaviano DavidCordeiro,NaylanaGuimarães,Sergio BotelhoJamacaru,Francisco Vagnaldo FechineMoraes Filho,Manoel Odorico de Carcinoma 256/Walker Animal Experimentation Hyperthermia/Induced Rats PURPOSE: To develop a model to evaluate the effects of focal pulsed ultrasound (US) waves as a source of heat for treatment of murine subcutaneous implanted Walker tumor. METHODS: An experimental, controlled, comparative study was conducted. Twenty male Wistar rats (160-300 g) randomized in 2 equal groups (G-1: Control and G-2: Hyperthermia) were inoculated with Walker-256 carcinosarcoma tumor. After 5 days G-2 rats were submitted to 45ºC hyperthermia. Heat was delivered directly to the tumor by an ultrasound (US) equipment (3 MHz frequency, 1,5W/cm³). Tumor temperature reached 45º C in 3 minutes and was maintained at this level for 5 minutes. Tumor volume was measured on days 5, 8, 11, 14 e 17 post inoculation in both groups. Unpaired t-test was used for comparison. P<0.05 was considered significant. RESULTS: Tumor volume was significantly greater in day 5 and decreased in days 11, 14 and 17 in treated rats. Rats treated with hyperthermia survived longer than control animals. On the 29th day following tumor inoculation, 40% of control rats and 77.78% of hyperthermia-treated rats remained alive. CONCLUSION: The proposed model is quite simple and may be used in less sophisticated laboratory settings for studying the effects of focal hyperthermia in the treatment of malignant implanted tumours or in survival studies.info:eu-repo/semantics/openAccessSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira v.26 suppl.1 20112011-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502011000700011en10.1590/S0102-86502011000700011 |
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Morano,José Antonio Carlos Otaviano David Cordeiro,Naylana Guimarães,Sergio Botelho Jamacaru,Francisco Vagnaldo Fechine Moraes Filho,Manoel Odorico de |
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Morano,José Antonio Carlos Otaviano David Cordeiro,Naylana Guimarães,Sergio Botelho Jamacaru,Francisco Vagnaldo Fechine Moraes Filho,Manoel Odorico de Experimental model of ultrasound thermotherapy in rats inoculated with Walker-236 tumor |
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Morano,José Antonio Carlos Otaviano David Cordeiro,Naylana Guimarães,Sergio Botelho Jamacaru,Francisco Vagnaldo Fechine Moraes Filho,Manoel Odorico de |
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Morano,José Antonio Carlos Otaviano David |
title |
Experimental model of ultrasound thermotherapy in rats inoculated with Walker-236 tumor |
title_short |
Experimental model of ultrasound thermotherapy in rats inoculated with Walker-236 tumor |
title_full |
Experimental model of ultrasound thermotherapy in rats inoculated with Walker-236 tumor |
title_fullStr |
Experimental model of ultrasound thermotherapy in rats inoculated with Walker-236 tumor |
title_full_unstemmed |
Experimental model of ultrasound thermotherapy in rats inoculated with Walker-236 tumor |
title_sort |
experimental model of ultrasound thermotherapy in rats inoculated with walker-236 tumor |
description |
PURPOSE: To develop a model to evaluate the effects of focal pulsed ultrasound (US) waves as a source of heat for treatment of murine subcutaneous implanted Walker tumor. METHODS: An experimental, controlled, comparative study was conducted. Twenty male Wistar rats (160-300 g) randomized in 2 equal groups (G-1: Control and G-2: Hyperthermia) were inoculated with Walker-256 carcinosarcoma tumor. After 5 days G-2 rats were submitted to 45ºC hyperthermia. Heat was delivered directly to the tumor by an ultrasound (US) equipment (3 MHz frequency, 1,5W/cm³). Tumor temperature reached 45º C in 3 minutes and was maintained at this level for 5 minutes. Tumor volume was measured on days 5, 8, 11, 14 e 17 post inoculation in both groups. Unpaired t-test was used for comparison. P<0.05 was considered significant. RESULTS: Tumor volume was significantly greater in day 5 and decreased in days 11, 14 and 17 in treated rats. Rats treated with hyperthermia survived longer than control animals. On the 29th day following tumor inoculation, 40% of control rats and 77.78% of hyperthermia-treated rats remained alive. CONCLUSION: The proposed model is quite simple and may be used in less sophisticated laboratory settings for studying the effects of focal hyperthermia in the treatment of malignant implanted tumours or in survival studies. |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
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2011 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502011000700011 |
work_keys_str_mv |
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1756400893850288128 |