Keratinocyte growth factor protected cultured human keratinocytes exposed to oxidative stress
PURPOSE: To evaluate effects of oxidative stress and supplementation of keratinocyte growth factor (KGF) on cultivated human keratinocytes. METHODS: Oxidative stress was produced through addition of hydrogen peroxide (H2O2) to the culture medium. Cultivated human keratinocytes were divided in 4 groups: Group control (G C), Group KGF (G KGF), Group H2O2 (G H2O2), Group H2O2 and KGF (G H2O2-KGF). Each experiment was accomplished with the same lineage cultivated keratinocytes, in triplicate. Cell viability was evaluated by trypan blue exclusion assay. RESULTS: The results showed that the culture medium supplemented with KGF presented a small rate of cell viability when compared to cells only in culture medium (p<0,001). It demonstrated that only the growth factor does not have protector effects for cells in vitro. However, in front of the oxidative stress produced by addition of hydrogen peroxide to the medium, KGF showed a beneficial effect, protecting cells when compared to the group that suffered hydrogen peroxide action but had not been exposed to KGF (p<0,001). CONCLUSION: KGF determined protection to the primary human keratinocytes exposed to oxidative stress.
Auteurs principaux: | Gragnani,Alfredo, Rossi,Marina Bertelli, Albuquerque,Janne Cely Medeiros, Brito,Gabriela Soares Silva, Aloise,Antonio Carlos, Ferreira,Lydia Masako |
---|---|
Format: | Digital revista |
Langue: | English |
Publié: |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2010
|
Accès en ligne: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502010000100019 |
Tags: |
Ajouter un tag
Pas de tags, Soyez le premier à ajouter un tag!
|
Documents similaires
-
Experimental model of cultured keratinocytes
par: Gragnani,Alfredo, et autres
Publié: (2003) -
Keratinocyte growth factor and the expression of wound-healing-related genes in primary human keratinocytes from burn patients
par: Chomiski,Verônica, et autres
Publié: (2016) -
Human beta defensin-4 and keratinocyte growth factor gene expression in cultured keratinocyte and fibroblasts of burned patients
par: Noronha,Silvana Aparecida Alves Corrêa de, et autres
Publié: (2014) -
Thermolysin in human cultured keratinocyte isolation
par: Gragnani,A., et autres
Publié: (2007) -
Keratinocyte growth factor, tumor necrosis factor-alpha and interleukin-1 beta gene expression in cultured fibroblasts and keratinocytes from burned patients
par: Gragnani,Alfredo, et autres
Publié: (2013)