Plasma metabolite changes in anestrous dairy cows with negative energy balance identified using 1H NMR technology

Plasma metabolite changes in anestrous dairy cows with negative energy balance identified using 1H NMR technology

ABSTRACT The objective of the present study was to investigate the different plasma metabolites between anestrus and estrus postpartum dairy cows and to provide a theoretical basis for prevention of anestrus in dairy farm cows. In the experiment, one hundred and sixty-seven Holstein dairy cows were selected with similar age and parity. According to the concentration of β-hydroxybutyric acid, non-esterified fatty acids and glucose in plasma during 14 to 21 days in milk, all dairy cows were determined as having a status of energy balance. According to the results of clinical symptom, rectal and B ultrasound examination at 60 to 90 days postpartum, these cows were divided into twenty estrus and twenty-four anestrus group, other dairy cows were removed. 1H nuclear magnetic resonance technology was utilized to detect the plasma metabolites changes and screen different plasma metabolites between anestrus and estrus cows. Ten different metabolites including alanine, glutamic acid, asparagine, creatine, choline, phosphocholine, glycerophosphocholine, low-density lipoprotein, and very-low-density lipoprotein were significantly decreased in anestrous cows compared with estrous cows. Metabolic pathway analyses indicated that differential metabolites were primarily involved in amino acid and glycerophospholipid metabolism. These metabolites and their enrichment pathways indicate that reduced steroid hormone synthesis precursors result in lower levels of estradiol and progesterone and cause anestrus in negative energy balance. These data provide a better understanding of the changes that may affect estrus of postpartum dairy cows at NEB status and lay the ground for further research.

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Main Authors: Zhang,F., Wang,Z., Zhao,C., Bai,Y., Wang,D., Yu,D., Xu,C., Xia,C.
Format: Digital revista
Language:English
Published: Universidade Federal de Minas Gerais, Escola de Veterinária 2021
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352021000400929
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spelling oai:scielo:S0102-093520210004009292021-08-20Plasma metabolite changes in anestrous dairy cows with negative energy balance identified using 1H NMR technologyZhang,F.Wang,Z.Zhao,C.Bai,Y.Wang,D.Yu,D.Xu,C.Xia,C. energy balance anestrus dairy cows metabolomics nuclear magnetic resonance ABSTRACT The objective of the present study was to investigate the different plasma metabolites between anestrus and estrus postpartum dairy cows and to provide a theoretical basis for prevention of anestrus in dairy farm cows. In the experiment, one hundred and sixty-seven Holstein dairy cows were selected with similar age and parity. According to the concentration of β-hydroxybutyric acid, non-esterified fatty acids and glucose in plasma during 14 to 21 days in milk, all dairy cows were determined as having a status of energy balance. According to the results of clinical symptom, rectal and B ultrasound examination at 60 to 90 days postpartum, these cows were divided into twenty estrus and twenty-four anestrus group, other dairy cows were removed. 1H nuclear magnetic resonance technology was utilized to detect the plasma metabolites changes and screen different plasma metabolites between anestrus and estrus cows. Ten different metabolites including alanine, glutamic acid, asparagine, creatine, choline, phosphocholine, glycerophosphocholine, low-density lipoprotein, and very-low-density lipoprotein were significantly decreased in anestrous cows compared with estrous cows. Metabolic pathway analyses indicated that differential metabolites were primarily involved in amino acid and glycerophospholipid metabolism. These metabolites and their enrichment pathways indicate that reduced steroid hormone synthesis precursors result in lower levels of estradiol and progesterone and cause anestrus in negative energy balance. These data provide a better understanding of the changes that may affect estrus of postpartum dairy cows at NEB status and lay the ground for further research.info:eu-repo/semantics/openAccessUniversidade Federal de Minas Gerais, Escola de VeterináriaArquivo Brasileiro de Medicina Veterinária e Zootecnia v.73 n.4 20212021-08-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352021000400929en10.1590/1678-4162-12123
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Zhang,F.
Wang,Z.
Zhao,C.
Bai,Y.
Wang,D.
Yu,D.
Xu,C.
Xia,C.
spellingShingle Zhang,F.
Wang,Z.
Zhao,C.
Bai,Y.
Wang,D.
Yu,D.
Xu,C.
Xia,C.
Plasma metabolite changes in anestrous dairy cows with negative energy balance identified using 1H NMR technology
author_facet Zhang,F.
Wang,Z.
Zhao,C.
Bai,Y.
Wang,D.
Yu,D.
Xu,C.
Xia,C.
author_sort Zhang,F.
title Plasma metabolite changes in anestrous dairy cows with negative energy balance identified using 1H NMR technology
title_short Plasma metabolite changes in anestrous dairy cows with negative energy balance identified using 1H NMR technology
title_full Plasma metabolite changes in anestrous dairy cows with negative energy balance identified using 1H NMR technology
title_fullStr Plasma metabolite changes in anestrous dairy cows with negative energy balance identified using 1H NMR technology
title_full_unstemmed Plasma metabolite changes in anestrous dairy cows with negative energy balance identified using 1H NMR technology
title_sort plasma metabolite changes in anestrous dairy cows with negative energy balance identified using 1h nmr technology
description ABSTRACT The objective of the present study was to investigate the different plasma metabolites between anestrus and estrus postpartum dairy cows and to provide a theoretical basis for prevention of anestrus in dairy farm cows. In the experiment, one hundred and sixty-seven Holstein dairy cows were selected with similar age and parity. According to the concentration of β-hydroxybutyric acid, non-esterified fatty acids and glucose in plasma during 14 to 21 days in milk, all dairy cows were determined as having a status of energy balance. According to the results of clinical symptom, rectal and B ultrasound examination at 60 to 90 days postpartum, these cows were divided into twenty estrus and twenty-four anestrus group, other dairy cows were removed. 1H nuclear magnetic resonance technology was utilized to detect the plasma metabolites changes and screen different plasma metabolites between anestrus and estrus cows. Ten different metabolites including alanine, glutamic acid, asparagine, creatine, choline, phosphocholine, glycerophosphocholine, low-density lipoprotein, and very-low-density lipoprotein were significantly decreased in anestrous cows compared with estrous cows. Metabolic pathway analyses indicated that differential metabolites were primarily involved in amino acid and glycerophospholipid metabolism. These metabolites and their enrichment pathways indicate that reduced steroid hormone synthesis precursors result in lower levels of estradiol and progesterone and cause anestrus in negative energy balance. These data provide a better understanding of the changes that may affect estrus of postpartum dairy cows at NEB status and lay the ground for further research.
publisher Universidade Federal de Minas Gerais, Escola de Veterinária
publishDate 2021
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352021000400929
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