Allogenic platelet-rich plasma in induced ulcers in rat’s cornea

ABSTRACT The objective in this study was to evaluate the clinic effect of applying allogenic platelet-rich plasma (PRP) heated or not, for treating cornea ulcers, including the dosage of PDGF-BB in the cornea. The ulcers were induced, standardizing the left eye from 81 rats (Ratus norvegicus, albinus variety), assigned randomly into three groups (N=27): control group (CG) which did not receive any topic treatment; heated PRP group (GA) and PRP group (GP), which received topical treatment every eight hours for five days. Each group underwent evaluation at 24 hours (M1), three days (M3) and five days (M5). The clinical exam evaluated the opacity, vascularization and corneal repair. The corneal PDGF-BB was dosed through the ELISA method. The corneal opacity was decreased in PRP-treated animals (GA and GP) and corneal repair time reduced when compared to CG at M1 and M5. Furthermore, GP showed greater vascularization at M3 compared to M1. Applied allogenic PRP eye drops, heated or not, speed up corneal healing, and reduce corneal repair time. However, the corneal PDGF concentration was not altered in any of the treatments.

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Main Authors: Gandolfi,M.G., Brandão,C.V.S., Pellizon,C.H., Hirota,I.N., Barros,R., Hussein,A.C.B., Merlini,N.B., Takahira,R.K., Mesquita,L.R.
Format: Digital revista
Language:English
Published: Universidade Federal de Minas Gerais, Escola de Veterinária 2021
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352021000300613
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spelling oai:scielo:S0102-093520210003006132021-06-17Allogenic platelet-rich plasma in induced ulcers in rat’s corneaGandolfi,M.G.Brandão,C.V.S.Pellizon,C.H.Hirota,I.N.Barros,R.Hussein,A.C.B.Merlini,N.B.Takahira,R.K.Mesquita,L.R. heated platelet-rich plasma growth factor PDGF plasma rich in growth factors eye drops ABSTRACT The objective in this study was to evaluate the clinic effect of applying allogenic platelet-rich plasma (PRP) heated or not, for treating cornea ulcers, including the dosage of PDGF-BB in the cornea. The ulcers were induced, standardizing the left eye from 81 rats (Ratus norvegicus, albinus variety), assigned randomly into three groups (N=27): control group (CG) which did not receive any topic treatment; heated PRP group (GA) and PRP group (GP), which received topical treatment every eight hours for five days. Each group underwent evaluation at 24 hours (M1), three days (M3) and five days (M5). The clinical exam evaluated the opacity, vascularization and corneal repair. The corneal PDGF-BB was dosed through the ELISA method. The corneal opacity was decreased in PRP-treated animals (GA and GP) and corneal repair time reduced when compared to CG at M1 and M5. Furthermore, GP showed greater vascularization at M3 compared to M1. Applied allogenic PRP eye drops, heated or not, speed up corneal healing, and reduce corneal repair time. However, the corneal PDGF concentration was not altered in any of the treatments.info:eu-repo/semantics/openAccessUniversidade Federal de Minas Gerais, Escola de VeterináriaArquivo Brasileiro de Medicina Veterinária e Zootecnia v.73 n.3 20212021-05-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352021000300613en10.1590/1678-4162-12214
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countrycode BR
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libraryname SciELO
language English
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author Gandolfi,M.G.
Brandão,C.V.S.
Pellizon,C.H.
Hirota,I.N.
Barros,R.
Hussein,A.C.B.
Merlini,N.B.
Takahira,R.K.
Mesquita,L.R.
spellingShingle Gandolfi,M.G.
Brandão,C.V.S.
Pellizon,C.H.
Hirota,I.N.
Barros,R.
Hussein,A.C.B.
Merlini,N.B.
Takahira,R.K.
Mesquita,L.R.
Allogenic platelet-rich plasma in induced ulcers in rat’s cornea
author_facet Gandolfi,M.G.
Brandão,C.V.S.
Pellizon,C.H.
Hirota,I.N.
Barros,R.
Hussein,A.C.B.
Merlini,N.B.
Takahira,R.K.
Mesquita,L.R.
author_sort Gandolfi,M.G.
title Allogenic platelet-rich plasma in induced ulcers in rat’s cornea
title_short Allogenic platelet-rich plasma in induced ulcers in rat’s cornea
title_full Allogenic platelet-rich plasma in induced ulcers in rat’s cornea
title_fullStr Allogenic platelet-rich plasma in induced ulcers in rat’s cornea
title_full_unstemmed Allogenic platelet-rich plasma in induced ulcers in rat’s cornea
title_sort allogenic platelet-rich plasma in induced ulcers in rat’s cornea
description ABSTRACT The objective in this study was to evaluate the clinic effect of applying allogenic platelet-rich plasma (PRP) heated or not, for treating cornea ulcers, including the dosage of PDGF-BB in the cornea. The ulcers were induced, standardizing the left eye from 81 rats (Ratus norvegicus, albinus variety), assigned randomly into three groups (N=27): control group (CG) which did not receive any topic treatment; heated PRP group (GA) and PRP group (GP), which received topical treatment every eight hours for five days. Each group underwent evaluation at 24 hours (M1), three days (M3) and five days (M5). The clinical exam evaluated the opacity, vascularization and corneal repair. The corneal PDGF-BB was dosed through the ELISA method. The corneal opacity was decreased in PRP-treated animals (GA and GP) and corneal repair time reduced when compared to CG at M1 and M5. Furthermore, GP showed greater vascularization at M3 compared to M1. Applied allogenic PRP eye drops, heated or not, speed up corneal healing, and reduce corneal repair time. However, the corneal PDGF concentration was not altered in any of the treatments.
publisher Universidade Federal de Minas Gerais, Escola de Veterinária
publishDate 2021
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352021000300613
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