Detection of mutations within exons 4 to 8 of the p53 tumor suppressor gene in canine mammary glands
Fifteen female canines with mammary tumors and 6 normal females were used to study mutations in exons 4 to 8 of the p53 gene. DNA samples from the tumors, respective adjacent normal mammary tissue and mammary glands from healthy animals were sequenced and analyzed for the presence of mutations. Mutations were found in 71.8% of the samples and the most frequent were missense mutations. The most attacked exons in the mammary tumor were 5, 7 and 8, with 23.4, 31.6 and 23.4% mutations, respectively. Canine mammary tumors are related to mutations in gene p53 and mutations mostly occur in the region of the protein that is linked to the DNA in the cell nucleus, which can change the functionality of the cell and propitiate tumor growth. Despite being macroscopically normal, the mammary tissue adjacent to the tumors has mutations that can lead to recurrence if not removed together with the tumor.
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Universidade Federal de Minas Gerais, Escola de Veterinária
2012
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oai:scielo:S0102-093520120002000132012-05-07Detection of mutations within exons 4 to 8 of the p53 tumor suppressor gene in canine mammary glandsSouza,D.M.B.Barros,M.G.O.Silva,J.S.C.Silva,M.B.Coleto,Z.F.Jimenez,G.C.Adrião,M.Wischral,A. bitch DNA mamma molecular biology neoplasm PCR Fifteen female canines with mammary tumors and 6 normal females were used to study mutations in exons 4 to 8 of the p53 gene. DNA samples from the tumors, respective adjacent normal mammary tissue and mammary glands from healthy animals were sequenced and analyzed for the presence of mutations. Mutations were found in 71.8% of the samples and the most frequent were missense mutations. The most attacked exons in the mammary tumor were 5, 7 and 8, with 23.4, 31.6 and 23.4% mutations, respectively. Canine mammary tumors are related to mutations in gene p53 and mutations mostly occur in the region of the protein that is linked to the DNA in the cell nucleus, which can change the functionality of the cell and propitiate tumor growth. Despite being macroscopically normal, the mammary tissue adjacent to the tumors has mutations that can lead to recurrence if not removed together with the tumor.info:eu-repo/semantics/openAccessUniversidade Federal de Minas Gerais, Escola de VeterináriaArquivo Brasileiro de Medicina Veterinária e Zootecnia v.64 n.2 20122012-04-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000200013en10.1590/S0102-09352012000200013 |
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Souza,D.M.B. Barros,M.G.O. Silva,J.S.C. Silva,M.B. Coleto,Z.F. Jimenez,G.C. Adrião,M. Wischral,A. |
| spellingShingle |
Souza,D.M.B. Barros,M.G.O. Silva,J.S.C. Silva,M.B. Coleto,Z.F. Jimenez,G.C. Adrião,M. Wischral,A. Detection of mutations within exons 4 to 8 of the p53 tumor suppressor gene in canine mammary glands |
| author_facet |
Souza,D.M.B. Barros,M.G.O. Silva,J.S.C. Silva,M.B. Coleto,Z.F. Jimenez,G.C. Adrião,M. Wischral,A. |
| author_sort |
Souza,D.M.B. |
| title |
Detection of mutations within exons 4 to 8 of the p53 tumor suppressor gene in canine mammary glands |
| title_short |
Detection of mutations within exons 4 to 8 of the p53 tumor suppressor gene in canine mammary glands |
| title_full |
Detection of mutations within exons 4 to 8 of the p53 tumor suppressor gene in canine mammary glands |
| title_fullStr |
Detection of mutations within exons 4 to 8 of the p53 tumor suppressor gene in canine mammary glands |
| title_full_unstemmed |
Detection of mutations within exons 4 to 8 of the p53 tumor suppressor gene in canine mammary glands |
| title_sort |
detection of mutations within exons 4 to 8 of the p53 tumor suppressor gene in canine mammary glands |
| description |
Fifteen female canines with mammary tumors and 6 normal females were used to study mutations in exons 4 to 8 of the p53 gene. DNA samples from the tumors, respective adjacent normal mammary tissue and mammary glands from healthy animals were sequenced and analyzed for the presence of mutations. Mutations were found in 71.8% of the samples and the most frequent were missense mutations. The most attacked exons in the mammary tumor were 5, 7 and 8, with 23.4, 31.6 and 23.4% mutations, respectively. Canine mammary tumors are related to mutations in gene p53 and mutations mostly occur in the region of the protein that is linked to the DNA in the cell nucleus, which can change the functionality of the cell and propitiate tumor growth. Despite being macroscopically normal, the mammary tissue adjacent to the tumors has mutations that can lead to recurrence if not removed together with the tumor. |
| publisher |
Universidade Federal de Minas Gerais, Escola de Veterinária |
| publishDate |
2012 |
| url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000200013 |
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