Somatic embryogenesis and plant regeneration from zygotic embryo explants of onion

Somatic embryogenesis and plant regeneration from zygotic embryo explants of onion

ABSTRACT: The study was undertaken to examine the effect of two synthetic auxins on somatic embryogenesis and plant regeneration from mature embryo explants of 16 onion cultivars. Cotyledons were removed from the embryos and remaining portions were cultured on MS medium supplemented with 1.0 mg/L 2,4-D and 5.0 mg/L picloram alone or in combination (1.0 mg/L 2,4-D + 2.5 mg/L picloram) to produce embryogenic callus. MS medium supplemented with 5.0 mg/L picloram was found to be the best one for both embryogenic callus induction (85%) and callus diameter (3.8 mm). Of the 16 cultivars studied, Yeoeuijuhwang exhibited the lowest frequency of embryogenic callus induction (50.5%), whereas all the other 15 cultivars showed more than 60% embryogenic callus induction. Scanning electron micrograph (SEM) analysis of embryogenic callus showed all stages of somatic embryos such as globular, scuetellar and coleoptilar. Plant regeneration was significantly affected by the composition of embryogenic callus induction medium. The greatest frequency of somatic embryo conversion was obtained from embryogenic callus developed in MS medium with 2,4-D (70.1%) followed by picloram (38.9%) and 2,4-D + picloram (34.5%). The germinated plantlets were further developed on the half-strength MS medium containing 3% sucrose and were acclimatized in the culture room with 98% survival rate.

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Auteurs principaux: Sivanesan,Iyyakkannu, Kyoung,Kim E, Kyoung,Kim M, Young,Ko E, Park,Se W
Format: Digital revista
Langue:English
Publié: Associação Brasileira de Horticultura 2015
Accès en ligne:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362015000400441
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spelling oai:scielo:S0102-053620150004004412016-01-07Somatic embryogenesis and plant regeneration from zygotic embryo explants of onionSivanesan,IyyakkannuKyoung,Kim EKyoung,Kim MYoung,Ko EPark,Se W Allium cepa auxin embryogenic callus in vitro propagation picloram. ABSTRACT: The study was undertaken to examine the effect of two synthetic auxins on somatic embryogenesis and plant regeneration from mature embryo explants of 16 onion cultivars. Cotyledons were removed from the embryos and remaining portions were cultured on MS medium supplemented with 1.0 mg/L 2,4-D and 5.0 mg/L picloram alone or in combination (1.0 mg/L 2,4-D + 2.5 mg/L picloram) to produce embryogenic callus. MS medium supplemented with 5.0 mg/L picloram was found to be the best one for both embryogenic callus induction (85%) and callus diameter (3.8 mm). Of the 16 cultivars studied, Yeoeuijuhwang exhibited the lowest frequency of embryogenic callus induction (50.5%), whereas all the other 15 cultivars showed more than 60% embryogenic callus induction. Scanning electron micrograph (SEM) analysis of embryogenic callus showed all stages of somatic embryos such as globular, scuetellar and coleoptilar. Plant regeneration was significantly affected by the composition of embryogenic callus induction medium. The greatest frequency of somatic embryo conversion was obtained from embryogenic callus developed in MS medium with 2,4-D (70.1%) followed by picloram (38.9%) and 2,4-D + picloram (34.5%). The germinated plantlets were further developed on the half-strength MS medium containing 3% sucrose and were acclimatized in the culture room with 98% survival rate.info:eu-repo/semantics/openAccessAssociação Brasileira de HorticulturaHorticultura Brasileira v.33 n.4 20152015-12-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362015000400441en10.1590/S0102-053620150000400006
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country Brasil
countrycode BR
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region America del Sur
libraryname SciELO
language English
format Digital
author Sivanesan,Iyyakkannu
Kyoung,Kim E
Kyoung,Kim M
Young,Ko E
Park,Se W
spellingShingle Sivanesan,Iyyakkannu
Kyoung,Kim E
Kyoung,Kim M
Young,Ko E
Park,Se W
Somatic embryogenesis and plant regeneration from zygotic embryo explants of onion
author_facet Sivanesan,Iyyakkannu
Kyoung,Kim E
Kyoung,Kim M
Young,Ko E
Park,Se W
author_sort Sivanesan,Iyyakkannu
title Somatic embryogenesis and plant regeneration from zygotic embryo explants of onion
title_short Somatic embryogenesis and plant regeneration from zygotic embryo explants of onion
title_full Somatic embryogenesis and plant regeneration from zygotic embryo explants of onion
title_fullStr Somatic embryogenesis and plant regeneration from zygotic embryo explants of onion
title_full_unstemmed Somatic embryogenesis and plant regeneration from zygotic embryo explants of onion
title_sort somatic embryogenesis and plant regeneration from zygotic embryo explants of onion
description ABSTRACT: The study was undertaken to examine the effect of two synthetic auxins on somatic embryogenesis and plant regeneration from mature embryo explants of 16 onion cultivars. Cotyledons were removed from the embryos and remaining portions were cultured on MS medium supplemented with 1.0 mg/L 2,4-D and 5.0 mg/L picloram alone or in combination (1.0 mg/L 2,4-D + 2.5 mg/L picloram) to produce embryogenic callus. MS medium supplemented with 5.0 mg/L picloram was found to be the best one for both embryogenic callus induction (85%) and callus diameter (3.8 mm). Of the 16 cultivars studied, Yeoeuijuhwang exhibited the lowest frequency of embryogenic callus induction (50.5%), whereas all the other 15 cultivars showed more than 60% embryogenic callus induction. Scanning electron micrograph (SEM) analysis of embryogenic callus showed all stages of somatic embryos such as globular, scuetellar and coleoptilar. Plant regeneration was significantly affected by the composition of embryogenic callus induction medium. The greatest frequency of somatic embryo conversion was obtained from embryogenic callus developed in MS medium with 2,4-D (70.1%) followed by picloram (38.9%) and 2,4-D + picloram (34.5%). The germinated plantlets were further developed on the half-strength MS medium containing 3% sucrose and were acclimatized in the culture room with 98% survival rate.
publisher Associação Brasileira de Horticultura
publishDate 2015
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362015000400441
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AT kyoungkime somaticembryogenesisandplantregenerationfromzygoticembryoexplantsofonion
AT kyoungkimm somaticembryogenesisandplantregenerationfromzygoticembryoexplantsofonion
AT youngkoe somaticembryogenesisandplantregenerationfromzygoticembryoexplantsofonion
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