Calcium homeostasis behavior and cardiac function on left ventricular remodeling by pressure overload

Calcium homeostasis behavior and cardiac function on left ventricular remodeling by pressure overload

Sarcoplasmic reticulum Ca2+-ATPase (SERCA2a) and sarcolemmal Na+/Ca2+ exchanger (NCX1) structures are involved in heart cell Ca2+ homeostasis. Previous studies have shown discrepancies in their function and expression in heart failure. The goal of this study was to evaluate heart function and hypertrophied muscle Ca2+-handling protein behavior under pressure overload. Twenty male Wistar rats were divided into two groups: Aortic stenosis (AoS), induced by a clip placed at the beginning of the aorta, and Control (Sham). After 18 weeks, heart function and structure were evaluated by echocardiogram. Myocardial function was analyzed by isolated papillary muscle (IPM) at basal condition and Ca2+ protein functions were evaluated after post-pause contraction and blockage with cyclopiazonic acid in IPM. Ca2+-handling protein expression was studied by western blot (WB). Echocardiogram showed that AoS caused concentric hypertrophy with enhanced ejection fraction and diastolic dysfunction inferred by dilated left atrium and increased relative wall thickness. IPM study showed developed tension was the same in both groups. AoS showed increased stiffness revealed by enhanced resting tension, and changes in Ca2+ homeostasis shown by calcium elevation and SERCA2a blockage maneuvers. WB revealed decreased NCX1, SERCA2a, and phosphorylated phospholambam (PLB) on serine-16 in AoS. AoS had left ventricular hypertrophy and diastolic dysfunction compared to Sham; this could be related to our findings regarding calcium homeostasis behavior: deficit in NCX1, SERCA2a, and phosphorylated PLB on serine-16.

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Main Authors: Mazeto,I.F.S., Okoshi,K., Silveira,C.F.S.M.P., Sant'Ana,P.G., Silva,V.L. da, Mota,G.A.F., Souza,S.L.B. de, Vileigas,D.F., Padovani,C.R., Cicogna,A.C.
Format: Digital revista
Language:English
Published: Associação Brasileira de Divulgação Científica 2021
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021000400601
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spelling oai:scielo:S0100-879X20210004006012021-02-19Calcium homeostasis behavior and cardiac function on left ventricular remodeling by pressure overloadMazeto,I.F.S.Okoshi,K.Silveira,C.F.S.M.P.Sant'Ana,P.G.Silva,V.L. daMota,G.A.F.Souza,S.L.B. deVileigas,D.F.Padovani,C.R.Cicogna,A.C. Aortic stenosis Diastolic dysfunction Calcium-handling proteins Rats Pressure Sarcoplasmic reticulum Ca2+-ATPase (SERCA2a) and sarcolemmal Na+/Ca2+ exchanger (NCX1) structures are involved in heart cell Ca2+ homeostasis. Previous studies have shown discrepancies in their function and expression in heart failure. The goal of this study was to evaluate heart function and hypertrophied muscle Ca2+-handling protein behavior under pressure overload. Twenty male Wistar rats were divided into two groups: Aortic stenosis (AoS), induced by a clip placed at the beginning of the aorta, and Control (Sham). After 18 weeks, heart function and structure were evaluated by echocardiogram. Myocardial function was analyzed by isolated papillary muscle (IPM) at basal condition and Ca2+ protein functions were evaluated after post-pause contraction and blockage with cyclopiazonic acid in IPM. Ca2+-handling protein expression was studied by western blot (WB). Echocardiogram showed that AoS caused concentric hypertrophy with enhanced ejection fraction and diastolic dysfunction inferred by dilated left atrium and increased relative wall thickness. IPM study showed developed tension was the same in both groups. AoS showed increased stiffness revealed by enhanced resting tension, and changes in Ca2+ homeostasis shown by calcium elevation and SERCA2a blockage maneuvers. WB revealed decreased NCX1, SERCA2a, and phosphorylated phospholambam (PLB) on serine-16 in AoS. AoS had left ventricular hypertrophy and diastolic dysfunction compared to Sham; this could be related to our findings regarding calcium homeostasis behavior: deficit in NCX1, SERCA2a, and phosphorylated PLB on serine-16.info:eu-repo/semantics/openAccessAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research v.54 n.4 20212021-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021000400601en10.1590/1414-431x202010138
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country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
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region America del Sur
libraryname SciELO
language English
format Digital
author Mazeto,I.F.S.
Okoshi,K.
Silveira,C.F.S.M.P.
Sant'Ana,P.G.
Silva,V.L. da
Mota,G.A.F.
Souza,S.L.B. de
Vileigas,D.F.
Padovani,C.R.
Cicogna,A.C.
spellingShingle Mazeto,I.F.S.
Okoshi,K.
Silveira,C.F.S.M.P.
Sant'Ana,P.G.
Silva,V.L. da
Mota,G.A.F.
Souza,S.L.B. de
Vileigas,D.F.
Padovani,C.R.
Cicogna,A.C.
Calcium homeostasis behavior and cardiac function on left ventricular remodeling by pressure overload
author_facet Mazeto,I.F.S.
Okoshi,K.
Silveira,C.F.S.M.P.
Sant'Ana,P.G.
Silva,V.L. da
Mota,G.A.F.
Souza,S.L.B. de
Vileigas,D.F.
Padovani,C.R.
Cicogna,A.C.
author_sort Mazeto,I.F.S.
title Calcium homeostasis behavior and cardiac function on left ventricular remodeling by pressure overload
title_short Calcium homeostasis behavior and cardiac function on left ventricular remodeling by pressure overload
title_full Calcium homeostasis behavior and cardiac function on left ventricular remodeling by pressure overload
title_fullStr Calcium homeostasis behavior and cardiac function on left ventricular remodeling by pressure overload
title_full_unstemmed Calcium homeostasis behavior and cardiac function on left ventricular remodeling by pressure overload
title_sort calcium homeostasis behavior and cardiac function on left ventricular remodeling by pressure overload
description Sarcoplasmic reticulum Ca2+-ATPase (SERCA2a) and sarcolemmal Na+/Ca2+ exchanger (NCX1) structures are involved in heart cell Ca2+ homeostasis. Previous studies have shown discrepancies in their function and expression in heart failure. The goal of this study was to evaluate heart function and hypertrophied muscle Ca2+-handling protein behavior under pressure overload. Twenty male Wistar rats were divided into two groups: Aortic stenosis (AoS), induced by a clip placed at the beginning of the aorta, and Control (Sham). After 18 weeks, heart function and structure were evaluated by echocardiogram. Myocardial function was analyzed by isolated papillary muscle (IPM) at basal condition and Ca2+ protein functions were evaluated after post-pause contraction and blockage with cyclopiazonic acid in IPM. Ca2+-handling protein expression was studied by western blot (WB). Echocardiogram showed that AoS caused concentric hypertrophy with enhanced ejection fraction and diastolic dysfunction inferred by dilated left atrium and increased relative wall thickness. IPM study showed developed tension was the same in both groups. AoS showed increased stiffness revealed by enhanced resting tension, and changes in Ca2+ homeostasis shown by calcium elevation and SERCA2a blockage maneuvers. WB revealed decreased NCX1, SERCA2a, and phosphorylated phospholambam (PLB) on serine-16 in AoS. AoS had left ventricular hypertrophy and diastolic dysfunction compared to Sham; this could be related to our findings regarding calcium homeostasis behavior: deficit in NCX1, SERCA2a, and phosphorylated PLB on serine-16.
publisher Associação Brasileira de Divulgação Científica
publishDate 2021
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021000400601
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