Pros and cons of insulin administration on liver glucose metabolism in strength-trained healthy mice

Pros and cons of insulin administration on liver glucose metabolism in strength-trained healthy mice

Non-diabetic individuals use hormones like insulin to improve muscle strength and performance. However, as insulin also leads the liver and the adipose tissue to an anabolic state, the purpose of this study was to investigate the effects of insulin on liver metabolism in trained non-diabetic Swiss mice. The mice were divided into four groups: sedentary treated with saline (SS) or insulin (SI) and trained treated with saline (TS) or insulin (TI). Training was made in a vertical stair, at 90% of the maximum load, three times per week. Insulin (0.3 U/kg body weight) or saline were given intraperitoneally five times per week. After eight weeks, tissue and blood were collected and in situ liver perfusion with glycerol+lactate or alanine+glutamine (4 mM each) was carried out. The trained animals increased their muscle strength (+100%) and decreased body weight gain (–11%), subcutaneous fat (–42%), mesenteric fat (–45%), and peritoneal adipocyte size (–33%) compared with the sedentary groups. Insulin prevented the adipose effects of training (TI). The gastrocnemius muscle had greater density of muscle fibers (+60%) and less connective tissue in the trained groups. Liver glycogen was increased by insulin (SI +40% and TI +117%), as well as liver basal glucose release (TI +40%). Lactate and pyruvate release were reduced to a half by training. The greater gluconeogenesis from alanine+glutamine induced by training (TS +50%) was reversed by insulin (TI). Insulin administration had no additional effect on muscle strength and reversed some of the lipolytic and gluconeogenic effects of the resistance training. Therefore, insulin administration does not complement training in improving liver glucose metabolism.

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Main Authors: Pereira,V.A.R., Vedovelli,K.S., Muller,G.Y., Depieri,Y.F., Avelar,D.H.C.G., de Amo,A.H.E., Jimenes,D.R., Martins,J.N.L., Silvério,A.C., Gomes,C.R.G., Godoi,V.A.F., Pedrosa,M.M.D.
Format: Digital revista
Language:English
Published: Associação Brasileira de Divulgação Científica 2019
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000200605
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spelling oai:scielo:S0100-879X20190002006052019-03-18Pros and cons of insulin administration on liver glucose metabolism in strength-trained healthy micePereira,V.A.R.Vedovelli,K.S.Muller,G.Y.Depieri,Y.F.Avelar,D.H.C.G.de Amo,A.H.E.Jimenes,D.R.Martins,J.N.L.Silvério,A.C.Gomes,C.R.G.Godoi,V.A.F.Pedrosa,M.M.D. Muscle strength Gluconeogenesis Liver Adiposity Training Non-diabetic individuals use hormones like insulin to improve muscle strength and performance. However, as insulin also leads the liver and the adipose tissue to an anabolic state, the purpose of this study was to investigate the effects of insulin on liver metabolism in trained non-diabetic Swiss mice. The mice were divided into four groups: sedentary treated with saline (SS) or insulin (SI) and trained treated with saline (TS) or insulin (TI). Training was made in a vertical stair, at 90% of the maximum load, three times per week. Insulin (0.3 U/kg body weight) or saline were given intraperitoneally five times per week. After eight weeks, tissue and blood were collected and in situ liver perfusion with glycerol+lactate or alanine+glutamine (4 mM each) was carried out. The trained animals increased their muscle strength (+100%) and decreased body weight gain (–11%), subcutaneous fat (–42%), mesenteric fat (–45%), and peritoneal adipocyte size (–33%) compared with the sedentary groups. Insulin prevented the adipose effects of training (TI). The gastrocnemius muscle had greater density of muscle fibers (+60%) and less connective tissue in the trained groups. Liver glycogen was increased by insulin (SI +40% and TI +117%), as well as liver basal glucose release (TI +40%). Lactate and pyruvate release were reduced to a half by training. The greater gluconeogenesis from alanine+glutamine induced by training (TS +50%) was reversed by insulin (TI). Insulin administration had no additional effect on muscle strength and reversed some of the lipolytic and gluconeogenic effects of the resistance training. Therefore, insulin administration does not complement training in improving liver glucose metabolism.info:eu-repo/semantics/openAccessAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research v.52 n.2 20192019-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000200605en10.1590/1414-431x20187637
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libraryname SciELO
language English
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author Pereira,V.A.R.
Vedovelli,K.S.
Muller,G.Y.
Depieri,Y.F.
Avelar,D.H.C.G.
de Amo,A.H.E.
Jimenes,D.R.
Martins,J.N.L.
Silvério,A.C.
Gomes,C.R.G.
Godoi,V.A.F.
Pedrosa,M.M.D.
spellingShingle Pereira,V.A.R.
Vedovelli,K.S.
Muller,G.Y.
Depieri,Y.F.
Avelar,D.H.C.G.
de Amo,A.H.E.
Jimenes,D.R.
Martins,J.N.L.
Silvério,A.C.
Gomes,C.R.G.
Godoi,V.A.F.
Pedrosa,M.M.D.
Pros and cons of insulin administration on liver glucose metabolism in strength-trained healthy mice
author_facet Pereira,V.A.R.
Vedovelli,K.S.
Muller,G.Y.
Depieri,Y.F.
Avelar,D.H.C.G.
de Amo,A.H.E.
Jimenes,D.R.
Martins,J.N.L.
Silvério,A.C.
Gomes,C.R.G.
Godoi,V.A.F.
Pedrosa,M.M.D.
author_sort Pereira,V.A.R.
title Pros and cons of insulin administration on liver glucose metabolism in strength-trained healthy mice
title_short Pros and cons of insulin administration on liver glucose metabolism in strength-trained healthy mice
title_full Pros and cons of insulin administration on liver glucose metabolism in strength-trained healthy mice
title_fullStr Pros and cons of insulin administration on liver glucose metabolism in strength-trained healthy mice
title_full_unstemmed Pros and cons of insulin administration on liver glucose metabolism in strength-trained healthy mice
title_sort pros and cons of insulin administration on liver glucose metabolism in strength-trained healthy mice
description Non-diabetic individuals use hormones like insulin to improve muscle strength and performance. However, as insulin also leads the liver and the adipose tissue to an anabolic state, the purpose of this study was to investigate the effects of insulin on liver metabolism in trained non-diabetic Swiss mice. The mice were divided into four groups: sedentary treated with saline (SS) or insulin (SI) and trained treated with saline (TS) or insulin (TI). Training was made in a vertical stair, at 90% of the maximum load, three times per week. Insulin (0.3 U/kg body weight) or saline were given intraperitoneally five times per week. After eight weeks, tissue and blood were collected and in situ liver perfusion with glycerol+lactate or alanine+glutamine (4 mM each) was carried out. The trained animals increased their muscle strength (+100%) and decreased body weight gain (–11%), subcutaneous fat (–42%), mesenteric fat (–45%), and peritoneal adipocyte size (–33%) compared with the sedentary groups. Insulin prevented the adipose effects of training (TI). The gastrocnemius muscle had greater density of muscle fibers (+60%) and less connective tissue in the trained groups. Liver glycogen was increased by insulin (SI +40% and TI +117%), as well as liver basal glucose release (TI +40%). Lactate and pyruvate release were reduced to a half by training. The greater gluconeogenesis from alanine+glutamine induced by training (TS +50%) was reversed by insulin (TI). Insulin administration had no additional effect on muscle strength and reversed some of the lipolytic and gluconeogenic effects of the resistance training. Therefore, insulin administration does not complement training in improving liver glucose metabolism.
publisher Associação Brasileira de Divulgação Científica
publishDate 2019
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000200605
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