Phytohemagglutinin improves the development and ultrastructure of in vitro-cultured goat (Capra hircus) preantral follicles
The objective this study was to determine the effect of phytohemagglutinin (PHA) on survival, growth and gene expression in caprine secondary follicles culturedin vitro. Secondary follicles (∼0.2 mm) were isolated from the cortex of caprine ovaries and cultured individually for 6 days in α-MEM+ supplemented with PHA (0, 1, 10, 50, 100, or 200 µg/mL). After 6 days of culture, follicle diameter and survival, antrum formation, ultrastructure and expression of mRNA for FSH receptors (FSH-R), proliferating cell nuclear antigen (PCNA), and neuronal nitric oxide synthase were determined. All treatments maintained follicular survival [α-MEM+ (94.59%); 1 µg/mL PHA (96.43%); 10 µg/mL PHA (84.85%); 50 µg/mL PHA (85.29%); 100 µg/mL PHA (88.57%), and 200 µg/mL PHA (87.50)], but the presence of 10 µg/mL PHA in the culture medium increased the antrum formation rate (21.21%) when compared with control (5.41%, P < 0.05) and ensured the maintenance of oocyte and granulosa cell ultrastructures after 6 days of culture. The expression of mRNA for FSH-R (2.7 ± 0.1) and PCNA (4.4 ± 0.2) was also significantly increased in follicles cultured with 10 µg/mL PHA in relation to those cultured in α-MEM+ (1.0 ± 0.1). In conclusion, supplementation of culture medium with 10 µg/mL PHA maintains the follicular viability and ultrastructure, and promotes the formation of antral cavity after 6 days of culture in vitro.
Main Authors: | Cunha,E.V., Costa,J.J.N., Rossi,R.O.D.S., Silva,A.W.B., Passos,J.R.S., Portela,A.M.L.R., Pereira,D.C.S.T., Donato,M.A.M., Campello,C.C., Saraiva,M.V.A., Peixoto,C.A., Silva,J.R.V., Santos,R.P. |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Associação Brasileira de Divulgação Científica
2013
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300245 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles
by: Domingues,S.F.S., et al.
Published: (2003) -
Sphingosine 1-phosphate promotes activation of aprine preantral follicle in vitro
by: Nóbrega Jr.,J.E., et al.
Published: (2014) -
Effect of somatotropin on survival and diameter of bovine preantral follicles
by: Silva,T.F., et al.
Published: (2019) -
Vitrification of bovine preantral follicles with dimethylsulfoxide and sucrose plus α-tocopherol
by: Jimenez,Carolina R., et al.
Published: (2016) -
Progesterone and Follicle Stimulating Hormone interact and promote goat preantral follicles survival and development in vitro
by: Lima-Verde,Isabel B., et al.
Published: (2012)