Desenvolvimento e validação de método analítico para determinação simultânea de lamivudina, zidovudina e nevirapina em comprimidos dose-fixa combinada por cromatografia líquida de alta eficiência

An analytical method has been developed and validated for the quantitation of lamivudine, zidovudine and nevirapine in the fixed-dose combination film-coated tablet by high performance liquid chromatography, in accordance with RE No. 899/2003, National Sanitary Surveillance Agency. It was based on an isocratic elution system with a potassium phosphate buffer pH 3.0: acetonitrile (60:40 v/v) mobile phase, C18, 250 x 46 mm column, 10µm particle size, λ 270 nm. The statistically evaluated results have shown that the method is specific, precise, accurate, and robust, ensuring the analytical safety of 3TC, AZT and NVP determination, which emerges as a new therapeutic alternative for antiretroviral treatment.

Bibliographic Details

Main Authors:	Lavra,Zênia Maria Maciel, Rolim Neto,Pedro José, Silva,Rosali Maria Ferreira da, Medeiros,Flávia Patrícia Morais de
Format:	Digital revista
Language:	Portuguese
Published:	Sociedade Brasileira de Química 2008
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422008000500005
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