Identification of zygotic and nucellar seedlings in polyembryonic mango cultivars
The objective of this work was to evaluate the occurrence of polyembryony in the mango cultivars Manila and Ataulfo, and to determine whether seedlings cultured in vitro are zygotic or nucelar. Percentage of polyembryony was calculated and the number of embryos in 100 seeds of each cultivar was recorded. 'Manila' exhibited 97% polyembryony with 3.4 embryos per seed, while 'Ataulfo' had 95% polyembryony with 3.2 embryos per seed. Later, 20 seeds of each cultivar were established in vitro, and it was analyzed those in which all embryos germinated (12 seeds from 'Manila' and 7 from 'Ataulfo'). DNA was extracted from seedling leaf tissue, and its origin was identified with 14 RAPD primers. The polymorphic markers recognized the seedlings of sexual origin in seven of nine 'Manila' polyembryonic seeds, and in four of seven 'Ataulfo' ones. Also, in polyembryonic seeds not all zygotic seedlings were produced by small embryos located at the micropyle.
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Embrapa Secretaria de Pesquisa e Desenvolvimento
2012
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oai:scielo:S0100-204X20120011000102013-01-14Identification of zygotic and nucellar seedlings in polyembryonic mango cultivarsOchoa,Elisa del Carmen MartínezAndrade-Rodríguez,MaríaRodríguez,Mario RocandioMonter,Angel Villegas Mangifera indica embryo culture funiculus molecular markers polymorphism The objective of this work was to evaluate the occurrence of polyembryony in the mango cultivars Manila and Ataulfo, and to determine whether seedlings cultured in vitro are zygotic or nucelar. Percentage of polyembryony was calculated and the number of embryos in 100 seeds of each cultivar was recorded. 'Manila' exhibited 97% polyembryony with 3.4 embryos per seed, while 'Ataulfo' had 95% polyembryony with 3.2 embryos per seed. Later, 20 seeds of each cultivar were established in vitro, and it was analyzed those in which all embryos germinated (12 seeds from 'Manila' and 7 from 'Ataulfo'). DNA was extracted from seedling leaf tissue, and its origin was identified with 14 RAPD primers. The polymorphic markers recognized the seedlings of sexual origin in seven of nine 'Manila' polyembryonic seeds, and in four of seven 'Ataulfo' ones. Also, in polyembryonic seeds not all zygotic seedlings were produced by small embryos located at the micropyle.info:eu-repo/semantics/openAccessEmbrapa Secretaria de Pesquisa e DesenvolvimentoPesquisa Agropecuária BrasileiraPesquisa Agropecuária Brasileira v.47 n.11 20122012-11-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012001100010en10.1590/S0100-204X2012001100010 |
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Ochoa,Elisa del Carmen Martínez Andrade-Rodríguez,María Rodríguez,Mario Rocandio Monter,Angel Villegas |
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Ochoa,Elisa del Carmen Martínez Andrade-Rodríguez,María Rodríguez,Mario Rocandio Monter,Angel Villegas Identification of zygotic and nucellar seedlings in polyembryonic mango cultivars |
author_facet |
Ochoa,Elisa del Carmen Martínez Andrade-Rodríguez,María Rodríguez,Mario Rocandio Monter,Angel Villegas |
author_sort |
Ochoa,Elisa del Carmen Martínez |
title |
Identification of zygotic and nucellar seedlings in polyembryonic mango cultivars |
title_short |
Identification of zygotic and nucellar seedlings in polyembryonic mango cultivars |
title_full |
Identification of zygotic and nucellar seedlings in polyembryonic mango cultivars |
title_fullStr |
Identification of zygotic and nucellar seedlings in polyembryonic mango cultivars |
title_full_unstemmed |
Identification of zygotic and nucellar seedlings in polyembryonic mango cultivars |
title_sort |
identification of zygotic and nucellar seedlings in polyembryonic mango cultivars |
description |
The objective of this work was to evaluate the occurrence of polyembryony in the mango cultivars Manila and Ataulfo, and to determine whether seedlings cultured in vitro are zygotic or nucelar. Percentage of polyembryony was calculated and the number of embryos in 100 seeds of each cultivar was recorded. 'Manila' exhibited 97% polyembryony with 3.4 embryos per seed, while 'Ataulfo' had 95% polyembryony with 3.2 embryos per seed. Later, 20 seeds of each cultivar were established in vitro, and it was analyzed those in which all embryos germinated (12 seeds from 'Manila' and 7 from 'Ataulfo'). DNA was extracted from seedling leaf tissue, and its origin was identified with 14 RAPD primers. The polymorphic markers recognized the seedlings of sexual origin in seven of nine 'Manila' polyembryonic seeds, and in four of seven 'Ataulfo' ones. Also, in polyembryonic seeds not all zygotic seedlings were produced by small embryos located at the micropyle. |
publisher |
Embrapa Secretaria de Pesquisa e Desenvolvimento |
publishDate |
2012 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012001100010 |
work_keys_str_mv |
AT ochoaelisadelcarmenmartinez identificationofzygoticandnucellarseedlingsinpolyembryonicmangocultivars AT andraderodriguezmaria identificationofzygoticandnucellarseedlingsinpolyembryonicmangocultivars AT rodriguezmariorocandio identificationofzygoticandnucellarseedlingsinpolyembryonicmangocultivars AT monterangelvillegas identificationofzygoticandnucellarseedlingsinpolyembryonicmangocultivars |
_version_ |
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