Rearing the southern green stink bug using an artificial dry diet and an artificial plant

Laboratory and greenhouse studies were conducted with an artificial dry diet to rear nymphs, and with an artificial plant as substrate for egg laying by the southern green stink bug, Nezara viridula (L.). The artificial diet was composed of: soybean protein (15 g); potato starch (7.5 g); dextrose (7.5 g); sucrose (2.5 g); cellulose (12.5 g); vitamin mixture (niacinamide 1 g, calcium pantothenate 1 g, thiamine 0.25 g, riboflavin 0.5 g, pyridoxine 0.25 g, folic acid 0.25 g, biotin 0.02 mL, vitamin B12 1 g - added to 1,000 mL of distilled water) (5.0 mL); soybean oil (20 mL); wheat germ (17.9 g); and water (30 mL). Nymphs showed normal feeding behavior when fed on the artificial diet. Nymphal development time was longer than or similar to that of nymphs fed on soybean pods. Total nymphal mortality was low (ca. 30%), both for nymphs reared on the artificial diet, and for nymphs fed on soybean pods. At adult emergence, fresh body weights were significantly (P<0.01) less on the artificial diet than on soybean pods. Despite the lower adult survivorship and fecundity on artificial plants than on soybean plants, it was demonstrated for the first time that a model simulating a natural plant, can be used as a substrate for egg mass laying, in conjunction with the artificial diet.

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Main Authors: PANIZZI,ANTÔNIO RICARDO, PARRA,JOSÉ ROBERTO POSTALI, SANTOS,CLÁUDIA HIRT, CARVALHO,DIOGO RODRIGUES
Format: Digital revista
Language:English
Published: Embrapa Secretaria de Pesquisa e Desenvolvimento 2000
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2000000900002
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spelling oai:scielo:S0100-204X20000009000022001-06-13Rearing the southern green stink bug using an artificial dry diet and an artificial plantPANIZZI,ANTÔNIO RICARDOPARRA,JOSÉ ROBERTO POSTALISANTOS,CLÁUDIA HIRTCARVALHO,DIOGO RODRIGUES Pentatomidae Nezara viridula Glycine max nymphs oviposition rearing techniques Laboratory and greenhouse studies were conducted with an artificial dry diet to rear nymphs, and with an artificial plant as substrate for egg laying by the southern green stink bug, Nezara viridula (L.). The artificial diet was composed of: soybean protein (15 g); potato starch (7.5 g); dextrose (7.5 g); sucrose (2.5 g); cellulose (12.5 g); vitamin mixture (niacinamide 1 g, calcium pantothenate 1 g, thiamine 0.25 g, riboflavin 0.5 g, pyridoxine 0.25 g, folic acid 0.25 g, biotin 0.02 mL, vitamin B12 1 g - added to 1,000 mL of distilled water) (5.0 mL); soybean oil (20 mL); wheat germ (17.9 g); and water (30 mL). Nymphs showed normal feeding behavior when fed on the artificial diet. Nymphal development time was longer than or similar to that of nymphs fed on soybean pods. Total nymphal mortality was low (ca. 30%), both for nymphs reared on the artificial diet, and for nymphs fed on soybean pods. At adult emergence, fresh body weights were significantly (P<0.01) less on the artificial diet than on soybean pods. Despite the lower adult survivorship and fecundity on artificial plants than on soybean plants, it was demonstrated for the first time that a model simulating a natural plant, can be used as a substrate for egg mass laying, in conjunction with the artificial diet.info:eu-repo/semantics/openAccessEmbrapa Secretaria de Pesquisa e DesenvolvimentoPesquisa Agropecuária BrasileiraPesquisa Agropecuária Brasileira v.35 n.9 20002000-09-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2000000900002en10.1590/S0100-204X2000000900002
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country Brasil
countrycode BR
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libraryname SciELO
language English
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author PANIZZI,ANTÔNIO RICARDO
PARRA,JOSÉ ROBERTO POSTALI
SANTOS,CLÁUDIA HIRT
CARVALHO,DIOGO RODRIGUES
spellingShingle PANIZZI,ANTÔNIO RICARDO
PARRA,JOSÉ ROBERTO POSTALI
SANTOS,CLÁUDIA HIRT
CARVALHO,DIOGO RODRIGUES
Rearing the southern green stink bug using an artificial dry diet and an artificial plant
author_facet PANIZZI,ANTÔNIO RICARDO
PARRA,JOSÉ ROBERTO POSTALI
SANTOS,CLÁUDIA HIRT
CARVALHO,DIOGO RODRIGUES
author_sort PANIZZI,ANTÔNIO RICARDO
title Rearing the southern green stink bug using an artificial dry diet and an artificial plant
title_short Rearing the southern green stink bug using an artificial dry diet and an artificial plant
title_full Rearing the southern green stink bug using an artificial dry diet and an artificial plant
title_fullStr Rearing the southern green stink bug using an artificial dry diet and an artificial plant
title_full_unstemmed Rearing the southern green stink bug using an artificial dry diet and an artificial plant
title_sort rearing the southern green stink bug using an artificial dry diet and an artificial plant
description Laboratory and greenhouse studies were conducted with an artificial dry diet to rear nymphs, and with an artificial plant as substrate for egg laying by the southern green stink bug, Nezara viridula (L.). The artificial diet was composed of: soybean protein (15 g); potato starch (7.5 g); dextrose (7.5 g); sucrose (2.5 g); cellulose (12.5 g); vitamin mixture (niacinamide 1 g, calcium pantothenate 1 g, thiamine 0.25 g, riboflavin 0.5 g, pyridoxine 0.25 g, folic acid 0.25 g, biotin 0.02 mL, vitamin B12 1 g - added to 1,000 mL of distilled water) (5.0 mL); soybean oil (20 mL); wheat germ (17.9 g); and water (30 mL). Nymphs showed normal feeding behavior when fed on the artificial diet. Nymphal development time was longer than or similar to that of nymphs fed on soybean pods. Total nymphal mortality was low (ca. 30%), both for nymphs reared on the artificial diet, and for nymphs fed on soybean pods. At adult emergence, fresh body weights were significantly (P<0.01) less on the artificial diet than on soybean pods. Despite the lower adult survivorship and fecundity on artificial plants than on soybean plants, it was demonstrated for the first time that a model simulating a natural plant, can be used as a substrate for egg mass laying, in conjunction with the artificial diet.
publisher Embrapa Secretaria de Pesquisa e Desenvolvimento
publishDate 2000
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2000000900002
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