Zika virus RNA excretion in sweat with concomitant detection in other body fluid specimens
We evaluated sweat, blood and urine specimens obtained from an ongoing cohort study in Brazil. Samples were collected at pre-established intervals after the initial rash presentation and tested for Zika virus (ZIKV) RNA presence by real-time reverse transcriptase polymerase chain reaction (rRT-PCR). From 254 participants with confirmed infection, ZIKV RNA was detected in the sweat of 46 individuals (18.1%). Sweat presented a median cycle threshold (Ct) of 34.74 [interquartile range (IQR) 33.44-36.04], comparable to plasma (Ct 35.96 - IQR 33.29-36.69) and higher than urine (Ct 30.78 - IQR 28.72-33.22). Concomitant detection with other specimens was observed in 33 (72%) of 46 participants who had a positive result in sweat. These findings represent an unusual and not yet investigated virus shedding through eccrine glands.
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Instituto Oswaldo Cruz, Ministério da Saúde
2020
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oai:scielo:S0074-027620200001004242021-01-21Zika virus RNA excretion in sweat with concomitant detection in other body fluid specimensMenezes-Neto,ArmandoCastilho,Marcia da CostaCalvet,Guilherme AmaralKara,Edna OliveiraBôtto-Menezes,Camila Helena AguiarLacerda,Marcus Vinícius GuimarãesPereira,Gerson Fernando MendesGiozza,Silvana PereiraBermudez,Ximena Pamela DiazLima,Noemia SantanaModjarrad,KayvonBroutet,Nathaliede Filippis,Ana Maria BispoFranca,Rafael Freitas Oliveira Zika virus diagnosis persistence We evaluated sweat, blood and urine specimens obtained from an ongoing cohort study in Brazil. Samples were collected at pre-established intervals after the initial rash presentation and tested for Zika virus (ZIKV) RNA presence by real-time reverse transcriptase polymerase chain reaction (rRT-PCR). From 254 participants with confirmed infection, ZIKV RNA was detected in the sweat of 46 individuals (18.1%). Sweat presented a median cycle threshold (Ct) of 34.74 [interquartile range (IQR) 33.44-36.04], comparable to plasma (Ct 35.96 - IQR 33.29-36.69) and higher than urine (Ct 30.78 - IQR 28.72-33.22). Concomitant detection with other specimens was observed in 33 (72%) of 46 participants who had a positive result in sweat. These findings represent an unusual and not yet investigated virus shedding through eccrine glands.info:eu-repo/semantics/openAccessInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz v.115 20202020-01-01info:eu-repo/semantics/othertext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762020000100424en10.1590/0074-02760200339 |
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Menezes-Neto,Armando Castilho,Marcia da Costa Calvet,Guilherme Amaral Kara,Edna Oliveira Bôtto-Menezes,Camila Helena Aguiar Lacerda,Marcus Vinícius Guimarães Pereira,Gerson Fernando Mendes Giozza,Silvana Pereira Bermudez,Ximena Pamela Diaz Lima,Noemia Santana Modjarrad,Kayvon Broutet,Nathalie de Filippis,Ana Maria Bispo Franca,Rafael Freitas Oliveira |
spellingShingle |
Menezes-Neto,Armando Castilho,Marcia da Costa Calvet,Guilherme Amaral Kara,Edna Oliveira Bôtto-Menezes,Camila Helena Aguiar Lacerda,Marcus Vinícius Guimarães Pereira,Gerson Fernando Mendes Giozza,Silvana Pereira Bermudez,Ximena Pamela Diaz Lima,Noemia Santana Modjarrad,Kayvon Broutet,Nathalie de Filippis,Ana Maria Bispo Franca,Rafael Freitas Oliveira Zika virus RNA excretion in sweat with concomitant detection in other body fluid specimens |
author_facet |
Menezes-Neto,Armando Castilho,Marcia da Costa Calvet,Guilherme Amaral Kara,Edna Oliveira Bôtto-Menezes,Camila Helena Aguiar Lacerda,Marcus Vinícius Guimarães Pereira,Gerson Fernando Mendes Giozza,Silvana Pereira Bermudez,Ximena Pamela Diaz Lima,Noemia Santana Modjarrad,Kayvon Broutet,Nathalie de Filippis,Ana Maria Bispo Franca,Rafael Freitas Oliveira |
author_sort |
Menezes-Neto,Armando |
title |
Zika virus RNA excretion in sweat with concomitant detection in other body fluid specimens |
title_short |
Zika virus RNA excretion in sweat with concomitant detection in other body fluid specimens |
title_full |
Zika virus RNA excretion in sweat with concomitant detection in other body fluid specimens |
title_fullStr |
Zika virus RNA excretion in sweat with concomitant detection in other body fluid specimens |
title_full_unstemmed |
Zika virus RNA excretion in sweat with concomitant detection in other body fluid specimens |
title_sort |
zika virus rna excretion in sweat with concomitant detection in other body fluid specimens |
description |
We evaluated sweat, blood and urine specimens obtained from an ongoing cohort study in Brazil. Samples were collected at pre-established intervals after the initial rash presentation and tested for Zika virus (ZIKV) RNA presence by real-time reverse transcriptase polymerase chain reaction (rRT-PCR). From 254 participants with confirmed infection, ZIKV RNA was detected in the sweat of 46 individuals (18.1%). Sweat presented a median cycle threshold (Ct) of 34.74 [interquartile range (IQR) 33.44-36.04], comparable to plasma (Ct 35.96 - IQR 33.29-36.69) and higher than urine (Ct 30.78 - IQR 28.72-33.22). Concomitant detection with other specimens was observed in 33 (72%) of 46 participants who had a positive result in sweat. These findings represent an unusual and not yet investigated virus shedding through eccrine glands. |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
publishDate |
2020 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762020000100424 |
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