SARS-CoV-2 isolation from the first reported patients in Brazil and establishment of a coordinated task network
BACKGROUND Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was confirmed in Brazil in February 2020, the first cases were followed by an increase in the number of cases throughout the country, resulting in an important public health crisis that requires fast and coordinated responses. OBJECTIVES The objective of this work is to describe the isolation and propagation properties of SARS-CoV-2 isolates from the first confirmed cases of coronavirus disease 2019 (COVID-19) in Brazil. METHODS After diagnosis in patients that returned from Italy to the São Paulo city in late February by RT-PCR, SARS-CoV-2 isolates were obtained in cell cultures and characterised by full genome sequencing, electron microscopy and in vitro replication properties. FINDINGS The virus isolate was recovered from nasopharyngeal specimen, propagated in Vero cells (E6, CCL-81 and hSLAM), with clear cytopathic effects, and characterised by full genome sequencing, electron microscopy and in vitro replication properties. Virus stocks - viable (titre 2.11 × 106 TCID50/mL, titre 1.5 × 106 PFUs/mL) and inactivated from isolate SARS.CoV2/SP02.2020.HIAE.Br were prepared and set available to the public health authorities and the scientific community in Brazil and abroad. MAIN CONCLUSION We believe that the protocols for virus growth and studies here described and the distribution initiative may constitute a viable model for other developing countries, not only to help a rapid effective pandemic response, but also to facilitate and support basic scientific research.
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Instituto Oswaldo Cruz, Ministério da Saúde
2020
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oai:scielo:S0074-027620200001003442020-10-20SARS-CoV-2 isolation from the first reported patients in Brazil and establishment of a coordinated task networkAraujo,Danielle BastosMachado,Rafael Rahal GuaragnaAmgarten,Deyvid EmanuelMalta,Fernanda de Mellode Araujo,Gabriel GuaranyMonteiro,Cairo OliveiraCandido,Erika DonizettiSoares,Camila Pereirade Menezes,Fernando GattiPires,Ana Carolina CornachioniSantana,Rúbia Anita FerrazViana,Amanda de OliveiraDorlass,ErickThomazelli,LucianoFerreira,Luis Carlos de SousaBotosso,Viviane FongaroCarvalho,Cristiane Rodrigues GuzzoOliveira,Danielle Bruna LealPinho,João Renato RebelloDurigon,Edison Luiz SARS-CoV-2 COVID-19 virus isolation cell culture virus network BACKGROUND Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was confirmed in Brazil in February 2020, the first cases were followed by an increase in the number of cases throughout the country, resulting in an important public health crisis that requires fast and coordinated responses. OBJECTIVES The objective of this work is to describe the isolation and propagation properties of SARS-CoV-2 isolates from the first confirmed cases of coronavirus disease 2019 (COVID-19) in Brazil. METHODS After diagnosis in patients that returned from Italy to the São Paulo city in late February by RT-PCR, SARS-CoV-2 isolates were obtained in cell cultures and characterised by full genome sequencing, electron microscopy and in vitro replication properties. FINDINGS The virus isolate was recovered from nasopharyngeal specimen, propagated in Vero cells (E6, CCL-81 and hSLAM), with clear cytopathic effects, and characterised by full genome sequencing, electron microscopy and in vitro replication properties. Virus stocks - viable (titre 2.11 × 106 TCID50/mL, titre 1.5 × 106 PFUs/mL) and inactivated from isolate SARS.CoV2/SP02.2020.HIAE.Br were prepared and set available to the public health authorities and the scientific community in Brazil and abroad. MAIN CONCLUSION We believe that the protocols for virus growth and studies here described and the distribution initiative may constitute a viable model for other developing countries, not only to help a rapid effective pandemic response, but also to facilitate and support basic scientific research.info:eu-repo/semantics/openAccessInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz v.115 20202020-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762020000100344en10.1590/0074-02760200342 |
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Araujo,Danielle Bastos Machado,Rafael Rahal Guaragna Amgarten,Deyvid Emanuel Malta,Fernanda de Mello de Araujo,Gabriel Guarany Monteiro,Cairo Oliveira Candido,Erika Donizetti Soares,Camila Pereira de Menezes,Fernando Gatti Pires,Ana Carolina Cornachioni Santana,Rúbia Anita Ferraz Viana,Amanda de Oliveira Dorlass,Erick Thomazelli,Luciano Ferreira,Luis Carlos de Sousa Botosso,Viviane Fongaro Carvalho,Cristiane Rodrigues Guzzo Oliveira,Danielle Bruna Leal Pinho,João Renato Rebello Durigon,Edison Luiz |
spellingShingle |
Araujo,Danielle Bastos Machado,Rafael Rahal Guaragna Amgarten,Deyvid Emanuel Malta,Fernanda de Mello de Araujo,Gabriel Guarany Monteiro,Cairo Oliveira Candido,Erika Donizetti Soares,Camila Pereira de Menezes,Fernando Gatti Pires,Ana Carolina Cornachioni Santana,Rúbia Anita Ferraz Viana,Amanda de Oliveira Dorlass,Erick Thomazelli,Luciano Ferreira,Luis Carlos de Sousa Botosso,Viviane Fongaro Carvalho,Cristiane Rodrigues Guzzo Oliveira,Danielle Bruna Leal Pinho,João Renato Rebello Durigon,Edison Luiz SARS-CoV-2 isolation from the first reported patients in Brazil and establishment of a coordinated task network |
author_facet |
Araujo,Danielle Bastos Machado,Rafael Rahal Guaragna Amgarten,Deyvid Emanuel Malta,Fernanda de Mello de Araujo,Gabriel Guarany Monteiro,Cairo Oliveira Candido,Erika Donizetti Soares,Camila Pereira de Menezes,Fernando Gatti Pires,Ana Carolina Cornachioni Santana,Rúbia Anita Ferraz Viana,Amanda de Oliveira Dorlass,Erick Thomazelli,Luciano Ferreira,Luis Carlos de Sousa Botosso,Viviane Fongaro Carvalho,Cristiane Rodrigues Guzzo Oliveira,Danielle Bruna Leal Pinho,João Renato Rebello Durigon,Edison Luiz |
author_sort |
Araujo,Danielle Bastos |
title |
SARS-CoV-2 isolation from the first reported patients in Brazil and establishment of a coordinated task network |
title_short |
SARS-CoV-2 isolation from the first reported patients in Brazil and establishment of a coordinated task network |
title_full |
SARS-CoV-2 isolation from the first reported patients in Brazil and establishment of a coordinated task network |
title_fullStr |
SARS-CoV-2 isolation from the first reported patients in Brazil and establishment of a coordinated task network |
title_full_unstemmed |
SARS-CoV-2 isolation from the first reported patients in Brazil and establishment of a coordinated task network |
title_sort |
sars-cov-2 isolation from the first reported patients in brazil and establishment of a coordinated task network |
description |
BACKGROUND Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was confirmed in Brazil in February 2020, the first cases were followed by an increase in the number of cases throughout the country, resulting in an important public health crisis that requires fast and coordinated responses. OBJECTIVES The objective of this work is to describe the isolation and propagation properties of SARS-CoV-2 isolates from the first confirmed cases of coronavirus disease 2019 (COVID-19) in Brazil. METHODS After diagnosis in patients that returned from Italy to the São Paulo city in late February by RT-PCR, SARS-CoV-2 isolates were obtained in cell cultures and characterised by full genome sequencing, electron microscopy and in vitro replication properties. FINDINGS The virus isolate was recovered from nasopharyngeal specimen, propagated in Vero cells (E6, CCL-81 and hSLAM), with clear cytopathic effects, and characterised by full genome sequencing, electron microscopy and in vitro replication properties. Virus stocks - viable (titre 2.11 × 106 TCID50/mL, titre 1.5 × 106 PFUs/mL) and inactivated from isolate SARS.CoV2/SP02.2020.HIAE.Br were prepared and set available to the public health authorities and the scientific community in Brazil and abroad. MAIN CONCLUSION We believe that the protocols for virus growth and studies here described and the distribution initiative may constitute a viable model for other developing countries, not only to help a rapid effective pandemic response, but also to facilitate and support basic scientific research. |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
publishDate |
2020 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762020000100344 |
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