Diagnostic challenges of single plaque-like lesion paucibacillary leprosy
The diagnosis of single-lesion paucibacillary leprosy remains a challenge. Reviews by expert dermatopathologists and quantitative polymerase chain reaction (qPCR) results obtained from 66 single-plaque biopsy samples were compared. Histological findings were graded as high (HP), medium (MP) or low (LP) probability of leprosy or other dermatopathy (OD). Mycobacterium leprae-specific genes were detected using qPCR. The biopsies of 47 out of 57 clinically diagnosed patients who received multidrug therapy were classified as HP/MP, eight of which were qPCR negative. In the LP/OD (n = 19), two out of eight untreated patients showed positive qPCR results. In the absence of typical histopathological features, qPCR may be utilised to aid in final patient diagnosis, thus reducing overtreatment and delay in diagnosis.
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Instituto Oswaldo Cruz, Ministério da Saúde
2014
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oai:scielo:S0074-027620140007009442016-09-15Diagnostic challenges of single plaque-like lesion paucibacillary leprosyBarbieri,Raquel RodriguesSales,Anna MariaIllarramendi,XimenaMoraes,Milton OzórioNery,José Augusto da CostaMoreira,Suelen Justo MariaSarno,Euzenir NunesMachado,Alice de MirandaBozza,Fernando Augusto paucibacillary leprosy diagnosis quantitative polymerase chain reaction The diagnosis of single-lesion paucibacillary leprosy remains a challenge. Reviews by expert dermatopathologists and quantitative polymerase chain reaction (qPCR) results obtained from 66 single-plaque biopsy samples were compared. Histological findings were graded as high (HP), medium (MP) or low (LP) probability of leprosy or other dermatopathy (OD). Mycobacterium leprae-specific genes were detected using qPCR. The biopsies of 47 out of 57 clinically diagnosed patients who received multidrug therapy were classified as HP/MP, eight of which were qPCR negative. In the LP/OD (n = 19), two out of eight untreated patients showed positive qPCR results. In the absence of typical histopathological features, qPCR may be utilised to aid in final patient diagnosis, thus reducing overtreatment and delay in diagnosis.info:eu-repo/semantics/openAccessInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz v.109 n.7 20142014-11-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000700944en10.1590/0074-0276140212 |
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Barbieri,Raquel Rodrigues Sales,Anna Maria Illarramendi,Ximena Moraes,Milton Ozório Nery,José Augusto da Costa Moreira,Suelen Justo Maria Sarno,Euzenir Nunes Machado,Alice de Miranda Bozza,Fernando Augusto |
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Barbieri,Raquel Rodrigues Sales,Anna Maria Illarramendi,Ximena Moraes,Milton Ozório Nery,José Augusto da Costa Moreira,Suelen Justo Maria Sarno,Euzenir Nunes Machado,Alice de Miranda Bozza,Fernando Augusto Diagnostic challenges of single plaque-like lesion paucibacillary leprosy |
author_facet |
Barbieri,Raquel Rodrigues Sales,Anna Maria Illarramendi,Ximena Moraes,Milton Ozório Nery,José Augusto da Costa Moreira,Suelen Justo Maria Sarno,Euzenir Nunes Machado,Alice de Miranda Bozza,Fernando Augusto |
author_sort |
Barbieri,Raquel Rodrigues |
title |
Diagnostic challenges of single plaque-like lesion paucibacillary leprosy |
title_short |
Diagnostic challenges of single plaque-like lesion paucibacillary leprosy |
title_full |
Diagnostic challenges of single plaque-like lesion paucibacillary leprosy |
title_fullStr |
Diagnostic challenges of single plaque-like lesion paucibacillary leprosy |
title_full_unstemmed |
Diagnostic challenges of single plaque-like lesion paucibacillary leprosy |
title_sort |
diagnostic challenges of single plaque-like lesion paucibacillary leprosy |
description |
The diagnosis of single-lesion paucibacillary leprosy remains a challenge. Reviews by expert dermatopathologists and quantitative polymerase chain reaction (qPCR) results obtained from 66 single-plaque biopsy samples were compared. Histological findings were graded as high (HP), medium (MP) or low (LP) probability of leprosy or other dermatopathy (OD). Mycobacterium leprae-specific genes were detected using qPCR. The biopsies of 47 out of 57 clinically diagnosed patients who received multidrug therapy were classified as HP/MP, eight of which were qPCR negative. In the LP/OD (n = 19), two out of eight untreated patients showed positive qPCR results. In the absence of typical histopathological features, qPCR may be utilised to aid in final patient diagnosis, thus reducing overtreatment and delay in diagnosis. |
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Instituto Oswaldo Cruz, Ministério da Saúde |
publishDate |
2014 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000700944 |
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