Genotyping of two Neisseria gonorrhoeae fluroquinolone-resistant strains in the Brazilian Amazon Region
We report two ciprofloxacin and ofloxacin-resistant Neisseria gonorrhoeae strains that were isolated from the urethral discharge of male patients at the sexually transmitted diseases outpatient clinic of the Alfredo da Matta Foundation (Manaus, state of Amazonas, Brazil). The gonococci displayed minimal inhibitory concentrations (> 32.00 µg/mL) and three mutations in the quinolone resistance-determining region (S91F and D95G in GyrA and S87R in ParC). Both isolates were genotyped using N. gonorrhoeae multi-antigen sequence typing and the analysis showed that the ST225 which represented an emerging widespread multi-resistant clone that has also been associated with reduced susceptibility to ceftriaxone. We recommend continued surveillance of this pathogen to assess the efficacy of anti-gonococcal antibiotics in Brazil.
Main Authors: | , , , , , , , |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Instituto Oswaldo Cruz, Ministério da Saúde
2011
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000500018 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
oai:scielo:S0074-02762011000500018 |
---|---|
record_format |
ojs |
spelling |
oai:scielo:S0074-027620110005000182011-08-30Genotyping of two Neisseria gonorrhoeae fluroquinolone-resistant strains in the Brazilian Amazon RegionFerreira,William AntunesFerreira,Cristina MottaNaveca,Felipe GomesAlmeida,Nayanne Cristina Oliveira da SilvaVasconcelos,Waldemara de SouzaGomes,Jairo de SouzaSilva,Maria de Fátima Pinto daAlecrim,Maria das Graças Costa Neisseria gonorrhoeae fluroquinolone resistance gyrA and parC genotyping MAST We report two ciprofloxacin and ofloxacin-resistant Neisseria gonorrhoeae strains that were isolated from the urethral discharge of male patients at the sexually transmitted diseases outpatient clinic of the Alfredo da Matta Foundation (Manaus, state of Amazonas, Brazil). The gonococci displayed minimal inhibitory concentrations (> 32.00 µg/mL) and three mutations in the quinolone resistance-determining region (S91F and D95G in GyrA and S87R in ParC). Both isolates were genotyped using N. gonorrhoeae multi-antigen sequence typing and the analysis showed that the ST225 which represented an emerging widespread multi-resistant clone that has also been associated with reduced susceptibility to ceftriaxone. We recommend continued surveillance of this pathogen to assess the efficacy of anti-gonococcal antibiotics in Brazil.info:eu-repo/semantics/openAccessInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz v.106 n.5 20112011-08-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000500018en10.1590/S0074-02762011000500018 |
institution |
SCIELO |
collection |
OJS |
country |
Brasil |
countrycode |
BR |
component |
Revista |
access |
En linea |
databasecode |
rev-scielo-br |
tag |
revista |
region |
America del Sur |
libraryname |
SciELO |
language |
English |
format |
Digital |
author |
Ferreira,William Antunes Ferreira,Cristina Motta Naveca,Felipe Gomes Almeida,Nayanne Cristina Oliveira da Silva Vasconcelos,Waldemara de Souza Gomes,Jairo de Souza Silva,Maria de Fátima Pinto da Alecrim,Maria das Graças Costa |
spellingShingle |
Ferreira,William Antunes Ferreira,Cristina Motta Naveca,Felipe Gomes Almeida,Nayanne Cristina Oliveira da Silva Vasconcelos,Waldemara de Souza Gomes,Jairo de Souza Silva,Maria de Fátima Pinto da Alecrim,Maria das Graças Costa Genotyping of two Neisseria gonorrhoeae fluroquinolone-resistant strains in the Brazilian Amazon Region |
author_facet |
Ferreira,William Antunes Ferreira,Cristina Motta Naveca,Felipe Gomes Almeida,Nayanne Cristina Oliveira da Silva Vasconcelos,Waldemara de Souza Gomes,Jairo de Souza Silva,Maria de Fátima Pinto da Alecrim,Maria das Graças Costa |
author_sort |
Ferreira,William Antunes |
title |
Genotyping of two Neisseria gonorrhoeae fluroquinolone-resistant strains in the Brazilian Amazon Region |
title_short |
Genotyping of two Neisseria gonorrhoeae fluroquinolone-resistant strains in the Brazilian Amazon Region |
title_full |
Genotyping of two Neisseria gonorrhoeae fluroquinolone-resistant strains in the Brazilian Amazon Region |
title_fullStr |
Genotyping of two Neisseria gonorrhoeae fluroquinolone-resistant strains in the Brazilian Amazon Region |
title_full_unstemmed |
Genotyping of two Neisseria gonorrhoeae fluroquinolone-resistant strains in the Brazilian Amazon Region |
title_sort |
genotyping of two neisseria gonorrhoeae fluroquinolone-resistant strains in the brazilian amazon region |
description |
We report two ciprofloxacin and ofloxacin-resistant Neisseria gonorrhoeae strains that were isolated from the urethral discharge of male patients at the sexually transmitted diseases outpatient clinic of the Alfredo da Matta Foundation (Manaus, state of Amazonas, Brazil). The gonococci displayed minimal inhibitory concentrations (> 32.00 µg/mL) and three mutations in the quinolone resistance-determining region (S91F and D95G in GyrA and S87R in ParC). Both isolates were genotyped using N. gonorrhoeae multi-antigen sequence typing and the analysis showed that the ST225 which represented an emerging widespread multi-resistant clone that has also been associated with reduced susceptibility to ceftriaxone. We recommend continued surveillance of this pathogen to assess the efficacy of anti-gonococcal antibiotics in Brazil. |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
publishDate |
2011 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000500018 |
work_keys_str_mv |
AT ferreirawilliamantunes genotypingoftwoneisseriagonorrhoeaefluroquinoloneresistantstrainsinthebrazilianamazonregion AT ferreiracristinamotta genotypingoftwoneisseriagonorrhoeaefluroquinoloneresistantstrainsinthebrazilianamazonregion AT navecafelipegomes genotypingoftwoneisseriagonorrhoeaefluroquinoloneresistantstrainsinthebrazilianamazonregion AT almeidanayannecristinaoliveiradasilva genotypingoftwoneisseriagonorrhoeaefluroquinoloneresistantstrainsinthebrazilianamazonregion AT vasconceloswaldemaradesouza genotypingoftwoneisseriagonorrhoeaefluroquinoloneresistantstrainsinthebrazilianamazonregion AT gomesjairodesouza genotypingoftwoneisseriagonorrhoeaefluroquinoloneresistantstrainsinthebrazilianamazonregion AT silvamariadefatimapintoda genotypingoftwoneisseriagonorrhoeaefluroquinoloneresistantstrainsinthebrazilianamazonregion AT alecrimmariadasgracascosta genotypingoftwoneisseriagonorrhoeaefluroquinoloneresistantstrainsinthebrazilianamazonregion |
_version_ |
1756383696605151232 |