Isoenzyme profiles of Trypanosoma cruzi stocks from different areas of Paraguay
Twenty one Trypanosoma cruzi stocks from humans, domiciliary triatomines and one sylvatic animal of different areas of Paraguay were subjected to isoenzyme analysis. Thirteen enzyme systems (15 loci in total) were studied. MN cl2 (clonets 39) and SO34 cl4 (clonets 20) were used as references. Relationships between stocks were depicted by an UPGMA dendrogram constructed using the Jaccard´s distances matrix. Among the Paraguayan stocks 14 zymodemes were identified (Par1 to Par14), Par 5 being the most frequent. Polymorphism rate and clonal diversity were 0.73 and 0.93, respectively. Average number of alleles per polymorphic locus was 2.5 (range 2-4). These measurements show a high diversity, which is confirmed by the dendrogram topology. All stocks belong to the same lineage, as MN cl2 reference strain (T. cruzi II). Moreover three distinct subgroups were identified and two of them correspond to Brazilian and Bolivian zymodemes, respectively. The third subgroup, the most common in Paraguay, is related to Tulahuen stock. The large geographical distribution of some zymodemes agrees with the hypothesis of clonality for T. cruzi populations. However sample size was not adequate to detect genetic recombination in any single locality.
Main Authors: | Acosta,Nidia, Samudio,Margarita, López,Elsa, Vargas,Fernando, Yaksic,Nina, Brenière,Simone Frédérique, Rojas de Arias,Antonieta |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Instituto Oswaldo Cruz, Ministério da Saúde
2001
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000400015 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Trypanosoma cruzi isoenzyme pattern as an epidemiological tool
by: Romanha,A. J., et al.
Published: (1988) -
The microdistribution of isoenzymic strains of Trypanosoma cruzi in southern Bolivia; new isoenzyme profiles and further arguments against Mendelian sexuality
by: Tibayrenc, M, et al.
Published: (1984) -
Mexican Trypanosoma cruzi stocks: analysis of minicircle kDNA homologies by cross-hybridization
by: Bosseno,Marie-France, et al.
Published: (2000) -
Cepas de Trypanosoma cruzi en el Paraguay: Trypanosoma cruzi strains in Paraguay
by: Acosta,N, et al.
Published: (2013) -
Reservorios mamíferos del Trypanosoma cruzi en Paraguay: Mammalian reservoirs of Trypanosoma cruzi in Paraguay
by: Acosta,N, et al.
Published: (2013)