Enzyme-linked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigen

Enzyme-linked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigen

An enzyme-linked immunosorbent assay was standardized for the detection of cryptococcal antigen in serum and cerebrospinal fluid. The system was evaluated in clinical samples from patients infected by human immunodeficiency virus with and without previous cryptococcosis diagnosis. The evaluated system is highly sensitive and specific, and when it was compared with latex agglutination there were not significant differences. A standard curve with purified Cryptococcus neoformans antigen was settled down for the antigen quantification in positive samples.

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Main Authors: Illnait,Ma.Teresa, Vilaseca,Juan C, Fernández,Carlos M, Martínez,Gerardo F
Format: Digital revista
Language:English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 2001
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000200018
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spelling oai:scielo:S0074-027620010002000182001-03-28Enzyme-linked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigenIllnait,Ma.TeresaVilaseca,Juan CFernández,Carlos MMartínez,Gerardo F cryptococcal antigen antigen quantification antigen detection enzyme-linked immunosorbent assay-ELISA An enzyme-linked immunosorbent assay was standardized for the detection of cryptococcal antigen in serum and cerebrospinal fluid. The system was evaluated in clinical samples from patients infected by human immunodeficiency virus with and without previous cryptococcosis diagnosis. The evaluated system is highly sensitive and specific, and when it was compared with latex agglutination there were not significant differences. A standard curve with purified Cryptococcus neoformans antigen was settled down for the antigen quantification in positive samples.info:eu-repo/semantics/openAccessInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz v.96 n.2 20012001-02-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000200018en10.1590/S0074-02762001000200018
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country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Illnait,Ma.Teresa
Vilaseca,Juan C
Fernández,Carlos M
Martínez,Gerardo F
spellingShingle Illnait,Ma.Teresa
Vilaseca,Juan C
Fernández,Carlos M
Martínez,Gerardo F
Enzyme-linked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigen
author_facet Illnait,Ma.Teresa
Vilaseca,Juan C
Fernández,Carlos M
Martínez,Gerardo F
author_sort Illnait,Ma.Teresa
title Enzyme-linked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigen
title_short Enzyme-linked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigen
title_full Enzyme-linked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigen
title_fullStr Enzyme-linked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigen
title_full_unstemmed Enzyme-linked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigen
title_sort enzyme-linked immunosorbent assay for detection and quantification of cryptococcus neoformans antigen
description An enzyme-linked immunosorbent assay was standardized for the detection of cryptococcal antigen in serum and cerebrospinal fluid. The system was evaluated in clinical samples from patients infected by human immunodeficiency virus with and without previous cryptococcosis diagnosis. The evaluated system is highly sensitive and specific, and when it was compared with latex agglutination there were not significant differences. A standard curve with purified Cryptococcus neoformans antigen was settled down for the antigen quantification in positive samples.
publisher Instituto Oswaldo Cruz, Ministério da Saúde
publishDate 2001
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000200018
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AT vilasecajuanc enzymelinkedimmunosorbentassayfordetectionandquantificationofcryptococcusneoformansantigen
AT fernandezcarlosm enzymelinkedimmunosorbentassayfordetectionandquantificationofcryptococcusneoformansantigen
AT martinezgerardof enzymelinkedimmunosorbentassayfordetectionandquantificationofcryptococcusneoformansantigen
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