Morphological study of adult male worms of Schistosoma mansoni Sambon, 1907 by confocal laser scanning microscopy
Aiming to detail data obtained through brightfield microscopy (BM) on reproductive, excretory and digestive system, specimens of Schistosoma mansoni eight weeks old, were recovered from SW mice, stained with Langeron's carmine and analyzed under a confocal laser scanning microscope CLSM 410 (Carl Zeiss). The reproductive system presented a single and lobate testis, with intercommunications between the lobes without efferent duct. Supernumerary testicular lobe was amorphous and isolated from the normal ones. Collecting tubules (excretory ducts), followed by the excretory bladder, opening to the external media through the excretory pore, were observed at the posterior extremity of the body. In the digestive tract, a cecal swelling was noted at the junction that originates the single cecum. It was concluded that through confocal laser scanning microscopy, new interpretations of morphological structures of S. mansoni worms could be achieved, modifying adopted and current descriptions. The gonad consists of a single lobed testis, similar to that observed in some trematode species. Moreover, the same specimens can be observed either by BM or CLSM, considering that the latter causes only focal and limited damage in tissue structures.
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Instituto Oswaldo Cruz, Ministério da Saúde
1998
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oai:scielo:S0074-027619980007000602000-07-14Morphological study of adult male worms of Schistosoma mansoni Sambon, 1907 by confocal laser scanning microscopyMachado-Silva,José RobertoPelajo-Machado,MarceloLenzi,Henrique LeonelGomes,Delir Corrêa Schistosoma mansoni adult male morphology confocal laser scanning microscopy reproductive system Aiming to detail data obtained through brightfield microscopy (BM) on reproductive, excretory and digestive system, specimens of Schistosoma mansoni eight weeks old, were recovered from SW mice, stained with Langeron's carmine and analyzed under a confocal laser scanning microscope CLSM 410 (Carl Zeiss). The reproductive system presented a single and lobate testis, with intercommunications between the lobes without efferent duct. Supernumerary testicular lobe was amorphous and isolated from the normal ones. Collecting tubules (excretory ducts), followed by the excretory bladder, opening to the external media through the excretory pore, were observed at the posterior extremity of the body. In the digestive tract, a cecal swelling was noted at the junction that originates the single cecum. It was concluded that through confocal laser scanning microscopy, new interpretations of morphological structures of S. mansoni worms could be achieved, modifying adopted and current descriptions. The gonad consists of a single lobed testis, similar to that observed in some trematode species. Moreover, the same specimens can be observed either by BM or CLSM, considering that the latter causes only focal and limited damage in tissue structures.info:eu-repo/semantics/openAccessInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz v.93 suppl.1 19981998-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761998000700060en10.1590/S0074-02761998000700060 |
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Machado-Silva,José Roberto Pelajo-Machado,Marcelo Lenzi,Henrique Leonel Gomes,Delir Corrêa |
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Machado-Silva,José Roberto Pelajo-Machado,Marcelo Lenzi,Henrique Leonel Gomes,Delir Corrêa Morphological study of adult male worms of Schistosoma mansoni Sambon, 1907 by confocal laser scanning microscopy |
author_facet |
Machado-Silva,José Roberto Pelajo-Machado,Marcelo Lenzi,Henrique Leonel Gomes,Delir Corrêa |
author_sort |
Machado-Silva,José Roberto |
title |
Morphological study of adult male worms of Schistosoma mansoni Sambon, 1907 by confocal laser scanning microscopy |
title_short |
Morphological study of adult male worms of Schistosoma mansoni Sambon, 1907 by confocal laser scanning microscopy |
title_full |
Morphological study of adult male worms of Schistosoma mansoni Sambon, 1907 by confocal laser scanning microscopy |
title_fullStr |
Morphological study of adult male worms of Schistosoma mansoni Sambon, 1907 by confocal laser scanning microscopy |
title_full_unstemmed |
Morphological study of adult male worms of Schistosoma mansoni Sambon, 1907 by confocal laser scanning microscopy |
title_sort |
morphological study of adult male worms of schistosoma mansoni sambon, 1907 by confocal laser scanning microscopy |
description |
Aiming to detail data obtained through brightfield microscopy (BM) on reproductive, excretory and digestive system, specimens of Schistosoma mansoni eight weeks old, were recovered from SW mice, stained with Langeron's carmine and analyzed under a confocal laser scanning microscope CLSM 410 (Carl Zeiss). The reproductive system presented a single and lobate testis, with intercommunications between the lobes without efferent duct. Supernumerary testicular lobe was amorphous and isolated from the normal ones. Collecting tubules (excretory ducts), followed by the excretory bladder, opening to the external media through the excretory pore, were observed at the posterior extremity of the body. In the digestive tract, a cecal swelling was noted at the junction that originates the single cecum. It was concluded that through confocal laser scanning microscopy, new interpretations of morphological structures of S. mansoni worms could be achieved, modifying adopted and current descriptions. The gonad consists of a single lobed testis, similar to that observed in some trematode species. Moreover, the same specimens can be observed either by BM or CLSM, considering that the latter causes only focal and limited damage in tissue structures. |
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Instituto Oswaldo Cruz, Ministério da Saúde |
publishDate |
1998 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761998000700060 |
work_keys_str_mv |
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1756383310673608704 |