Oxidative stress induction by crude extract of Xylaria sp. triggers lethality in the larvae of Aedes aegypti (Diptera: Culicidae)

Oxidative stress induction by crude extract of Xylaria sp. triggers lethality in the larvae of Aedes aegypti (Diptera: Culicidae)

ABSTRACT Background: Aedes aegypti is currently controlled with synthetic larvicides; however, mosquitoes have become highly resistant to these larvicides and difficult to eradicate. Studies have shown that insecticides derived from fungal extracts have various mechanisms of action that reduce the risk of resistance in these mosquitoes. One possible mechanism is uncontrolled production of reactive oxygen species (ROS) in the larvae, which can cause changes at the cellular level. Thus, the crude extract of Xylaria sp. was evaluated to investigate the oxidative effect of this extract in A. aegypti larvae by quantifying the oxidative damage to proteins and lipids. Methods: The larvicidal potential of the crude extract of Xylaria sp. Was evaluated, and the extract was subsequently tested in human lung fibroblasts for cytotoxicity and ROS production. ROS level was quantified in the larvae that were killed following exposure to the extract in the larvicide test. Results: The crude extract of Xylaria sp. Caused cytotoxicity and induced ROS production in human lung fibroblasts and A. aegypti larvae, respectively. In the larvicide trial, the extract showed an LC50 of 264.456 ppm and an LC90 of 364.307 ppm, and was thus considered active. The extract showed greater oxidative damage to lipids and proteins, with LC90 values of 24.7 µmol MDA/L and 14.6278 ×10-3 nmol carbonyl/ mg protein, respectively. Conclusions: Crude extracts of Xylaria sp. induced oxidative stress that may have caused the mortality of A. aegypti larvae.

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Auteurs principaux: Costa,Maria Beatriz Silva, Simões,Rejane de Castro, Silva,Márcia de Jesus Amazonas da, Oliveira,André Correa de, Acho,Leonard Domingo Rosales, Lima,Emerson Silva, Tadei,Wanderli Pedro, Teles,Helder Lopes, Oliveira,Camila Martins de
Format: Digital revista
Langue:English
Publié: Sociedade Brasileira de Medicina Tropical - SBMT 2022
Accès en ligne:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822022000100312
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spelling oai:scielo:S0037-868220220001003122022-04-26Oxidative stress induction by crude extract of Xylaria sp. triggers lethality in the larvae of Aedes aegypti (Diptera: Culicidae)Costa,Maria Beatriz SilvaSimões,Rejane de CastroSilva,Márcia de Jesus Amazonas daOliveira,André Correa deAcho,Leonard Domingo RosalesLima,Emerson SilvaTadei,Wanderli PedroTeles,Helder LopesOliveira,Camila Martins de Cytotoxicity Carbonyl Dengue TBARS Endophytic fungus ABSTRACT Background: Aedes aegypti is currently controlled with synthetic larvicides; however, mosquitoes have become highly resistant to these larvicides and difficult to eradicate. Studies have shown that insecticides derived from fungal extracts have various mechanisms of action that reduce the risk of resistance in these mosquitoes. One possible mechanism is uncontrolled production of reactive oxygen species (ROS) in the larvae, which can cause changes at the cellular level. Thus, the crude extract of Xylaria sp. was evaluated to investigate the oxidative effect of this extract in A. aegypti larvae by quantifying the oxidative damage to proteins and lipids. Methods: The larvicidal potential of the crude extract of Xylaria sp. Was evaluated, and the extract was subsequently tested in human lung fibroblasts for cytotoxicity and ROS production. ROS level was quantified in the larvae that were killed following exposure to the extract in the larvicide test. Results: The crude extract of Xylaria sp. Caused cytotoxicity and induced ROS production in human lung fibroblasts and A. aegypti larvae, respectively. In the larvicide trial, the extract showed an LC50 of 264.456 ppm and an LC90 of 364.307 ppm, and was thus considered active. The extract showed greater oxidative damage to lipids and proteins, with LC90 values of 24.7 µmol MDA/L and 14.6278 ×10-3 nmol carbonyl/ mg protein, respectively. Conclusions: Crude extracts of Xylaria sp. induced oxidative stress that may have caused the mortality of A. aegypti larvae.info:eu-repo/semantics/openAccessSociedade Brasileira de Medicina Tropical - SBMTRevista da Sociedade Brasileira de Medicina Tropical v.55 20222022-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822022000100312en10.1590/0037-8682-0373-2021
institution SCIELO
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countrycode BR
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libraryname SciELO
language English
format Digital
author Costa,Maria Beatriz Silva
Simões,Rejane de Castro
Silva,Márcia de Jesus Amazonas da
Oliveira,André Correa de
Acho,Leonard Domingo Rosales
Lima,Emerson Silva
Tadei,Wanderli Pedro
Teles,Helder Lopes
Oliveira,Camila Martins de
spellingShingle Costa,Maria Beatriz Silva
Simões,Rejane de Castro
Silva,Márcia de Jesus Amazonas da
Oliveira,André Correa de
Acho,Leonard Domingo Rosales
Lima,Emerson Silva
Tadei,Wanderli Pedro
Teles,Helder Lopes
Oliveira,Camila Martins de
Oxidative stress induction by crude extract of Xylaria sp. triggers lethality in the larvae of Aedes aegypti (Diptera: Culicidae)
author_facet Costa,Maria Beatriz Silva
Simões,Rejane de Castro
Silva,Márcia de Jesus Amazonas da
Oliveira,André Correa de
Acho,Leonard Domingo Rosales
Lima,Emerson Silva
Tadei,Wanderli Pedro
Teles,Helder Lopes
Oliveira,Camila Martins de
author_sort Costa,Maria Beatriz Silva
title Oxidative stress induction by crude extract of Xylaria sp. triggers lethality in the larvae of Aedes aegypti (Diptera: Culicidae)
title_short Oxidative stress induction by crude extract of Xylaria sp. triggers lethality in the larvae of Aedes aegypti (Diptera: Culicidae)
title_full Oxidative stress induction by crude extract of Xylaria sp. triggers lethality in the larvae of Aedes aegypti (Diptera: Culicidae)
title_fullStr Oxidative stress induction by crude extract of Xylaria sp. triggers lethality in the larvae of Aedes aegypti (Diptera: Culicidae)
title_full_unstemmed Oxidative stress induction by crude extract of Xylaria sp. triggers lethality in the larvae of Aedes aegypti (Diptera: Culicidae)
title_sort oxidative stress induction by crude extract of xylaria sp. triggers lethality in the larvae of aedes aegypti (diptera: culicidae)
description ABSTRACT Background: Aedes aegypti is currently controlled with synthetic larvicides; however, mosquitoes have become highly resistant to these larvicides and difficult to eradicate. Studies have shown that insecticides derived from fungal extracts have various mechanisms of action that reduce the risk of resistance in these mosquitoes. One possible mechanism is uncontrolled production of reactive oxygen species (ROS) in the larvae, which can cause changes at the cellular level. Thus, the crude extract of Xylaria sp. was evaluated to investigate the oxidative effect of this extract in A. aegypti larvae by quantifying the oxidative damage to proteins and lipids. Methods: The larvicidal potential of the crude extract of Xylaria sp. Was evaluated, and the extract was subsequently tested in human lung fibroblasts for cytotoxicity and ROS production. ROS level was quantified in the larvae that were killed following exposure to the extract in the larvicide test. Results: The crude extract of Xylaria sp. Caused cytotoxicity and induced ROS production in human lung fibroblasts and A. aegypti larvae, respectively. In the larvicide trial, the extract showed an LC50 of 264.456 ppm and an LC90 of 364.307 ppm, and was thus considered active. The extract showed greater oxidative damage to lipids and proteins, with LC90 values of 24.7 µmol MDA/L and 14.6278 ×10-3 nmol carbonyl/ mg protein, respectively. Conclusions: Crude extracts of Xylaria sp. induced oxidative stress that may have caused the mortality of A. aegypti larvae.
publisher Sociedade Brasileira de Medicina Tropical - SBMT
publishDate 2022
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822022000100312
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