New molecular target for the phylogenetic identification of Leptospira species directly from clinical samples: an alternative gene to 16S rRNA

New molecular target for the phylogenetic identification of Leptospira species directly from clinical samples: an alternative gene to 16S rRNA

Abstract INTRODUCTION: Phylogenetic analysis of the 16S ribosomal gene initial region is used to identify Leptospira isolates at the species level from clinical samples. Unfortunately, this method cannot differentiate between some intermediates and saprophytic species. METHODS: We used comparative genomic analysis between 35 Leptospira species to find new molecular targets for Leptospira species identification. RESULTS: We proposed the use of the rpoC gene, encoding the DNA-directed RNA polymerase β-subunit, for identifying 35 Leptospira species. CONCLUSIONS: The rpoC gene can be a molecular target to identify the main species of the Leptospira genus directly from clinical samples.

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Main Authors: Julio,Rafael Guillermo Villarreal, Agudelo-Flórez,Piedad, López,Juan Álvaro, Sánchez,Ronald Guillermo Peláez
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Medicina Tropical - SBMT 2020
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822020000100635
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spelling oai:scielo:S0037-868220200001006352020-03-11New molecular target for the phylogenetic identification of Leptospira species directly from clinical samples: an alternative gene to 16S rRNAJulio,Rafael Guillermo VillarrealAgudelo-Flórez,PiedadLópez,Juan ÁlvaroSánchez,Ronald Guillermo Peláez Leptospira Species Phylogenetic Typing rpoC Abstract INTRODUCTION: Phylogenetic analysis of the 16S ribosomal gene initial region is used to identify Leptospira isolates at the species level from clinical samples. Unfortunately, this method cannot differentiate between some intermediates and saprophytic species. METHODS: We used comparative genomic analysis between 35 Leptospira species to find new molecular targets for Leptospira species identification. RESULTS: We proposed the use of the rpoC gene, encoding the DNA-directed RNA polymerase β-subunit, for identifying 35 Leptospira species. CONCLUSIONS: The rpoC gene can be a molecular target to identify the main species of the Leptospira genus directly from clinical samples.info:eu-repo/semantics/openAccessSociedade Brasileira de Medicina Tropical - SBMTRevista da Sociedade Brasileira de Medicina Tropical v.53 20202020-01-01info:eu-repo/semantics/othertext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822020000100635en10.1590/0037-8682-0333-2019
institution SCIELO
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country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Julio,Rafael Guillermo Villarreal
Agudelo-Flórez,Piedad
López,Juan Álvaro
Sánchez,Ronald Guillermo Peláez
spellingShingle Julio,Rafael Guillermo Villarreal
Agudelo-Flórez,Piedad
López,Juan Álvaro
Sánchez,Ronald Guillermo Peláez
New molecular target for the phylogenetic identification of Leptospira species directly from clinical samples: an alternative gene to 16S rRNA
author_facet Julio,Rafael Guillermo Villarreal
Agudelo-Flórez,Piedad
López,Juan Álvaro
Sánchez,Ronald Guillermo Peláez
author_sort Julio,Rafael Guillermo Villarreal
title New molecular target for the phylogenetic identification of Leptospira species directly from clinical samples: an alternative gene to 16S rRNA
title_short New molecular target for the phylogenetic identification of Leptospira species directly from clinical samples: an alternative gene to 16S rRNA
title_full New molecular target for the phylogenetic identification of Leptospira species directly from clinical samples: an alternative gene to 16S rRNA
title_fullStr New molecular target for the phylogenetic identification of Leptospira species directly from clinical samples: an alternative gene to 16S rRNA
title_full_unstemmed New molecular target for the phylogenetic identification of Leptospira species directly from clinical samples: an alternative gene to 16S rRNA
title_sort new molecular target for the phylogenetic identification of leptospira species directly from clinical samples: an alternative gene to 16s rrna
description Abstract INTRODUCTION: Phylogenetic analysis of the 16S ribosomal gene initial region is used to identify Leptospira isolates at the species level from clinical samples. Unfortunately, this method cannot differentiate between some intermediates and saprophytic species. METHODS: We used comparative genomic analysis between 35 Leptospira species to find new molecular targets for Leptospira species identification. RESULTS: We proposed the use of the rpoC gene, encoding the DNA-directed RNA polymerase β-subunit, for identifying 35 Leptospira species. CONCLUSIONS: The rpoC gene can be a molecular target to identify the main species of the Leptospira genus directly from clinical samples.
publisher Sociedade Brasileira de Medicina Tropical - SBMT
publishDate 2020
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822020000100635
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AT lopezjuanalvaro newmoleculartargetforthephylogeneticidentificationofleptospiraspeciesdirectlyfromclinicalsamplesanalternativegeneto16srrna
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