Stress conditions in the host induce persister cells and influence biofilm formation by Staphylococcus epidermidis RP62A
Abstract INTRODUCTION: Studies have demonstrated that pathogens react to the harsh conditions in human tissues by inducing mechanisms that promote survival. METHODS: Persistence and biofilm-forming ability were evaluated during stress conditions that mimic those in the host. RESULTS: Carbon-source availability had a positive effect on Staphylococcus epidermidis RP62A adhesion during hypoxia, accompanied by a decrease in pH. In contrast, iron limitation led to decreased surface-adherent biomass, accompanied by an increase medium acidification and lactate levels. Interestingly, iron starvation and hypoxia induced persister cells in planktonic culture. CONCLUSIONS: These findings highlight the role of host stress in the virulence of S. epidermidis.
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Sociedade Brasileira de Medicina Tropical - SBMT
2019
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oai:scielo:S0037-868220190001006082019-02-11Stress conditions in the host induce persister cells and influence biofilm formation by Staphylococcus epidermidis RP62AFraiha,Rafael OvandoPereira,Ana Paula RamosBrito,Eliana da Costa AlvarengaBorges,Clayton LuizParente,Ana Flávia AlvesPerdomo,Renata TrentinMacedo,Maria Ligia RodriguesWeber,Simone Schneider Persistence Starvation Virulence Abstract INTRODUCTION: Studies have demonstrated that pathogens react to the harsh conditions in human tissues by inducing mechanisms that promote survival. METHODS: Persistence and biofilm-forming ability were evaluated during stress conditions that mimic those in the host. RESULTS: Carbon-source availability had a positive effect on Staphylococcus epidermidis RP62A adhesion during hypoxia, accompanied by a decrease in pH. In contrast, iron limitation led to decreased surface-adherent biomass, accompanied by an increase medium acidification and lactate levels. Interestingly, iron starvation and hypoxia induced persister cells in planktonic culture. CONCLUSIONS: These findings highlight the role of host stress in the virulence of S. epidermidis.info:eu-repo/semantics/openAccessSociedade Brasileira de Medicina Tropical - SBMTRevista da Sociedade Brasileira de Medicina Tropical v.52 20192019-01-01info:eu-repo/semantics/othertext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822019000100608en10.1590/0037-8682-0001-2018 |
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Fraiha,Rafael Ovando Pereira,Ana Paula Ramos Brito,Eliana da Costa Alvarenga Borges,Clayton Luiz Parente,Ana Flávia Alves Perdomo,Renata Trentin Macedo,Maria Ligia Rodrigues Weber,Simone Schneider |
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Fraiha,Rafael Ovando Pereira,Ana Paula Ramos Brito,Eliana da Costa Alvarenga Borges,Clayton Luiz Parente,Ana Flávia Alves Perdomo,Renata Trentin Macedo,Maria Ligia Rodrigues Weber,Simone Schneider Stress conditions in the host induce persister cells and influence biofilm formation by Staphylococcus epidermidis RP62A |
author_facet |
Fraiha,Rafael Ovando Pereira,Ana Paula Ramos Brito,Eliana da Costa Alvarenga Borges,Clayton Luiz Parente,Ana Flávia Alves Perdomo,Renata Trentin Macedo,Maria Ligia Rodrigues Weber,Simone Schneider |
author_sort |
Fraiha,Rafael Ovando |
title |
Stress conditions in the host induce persister cells and influence biofilm formation by Staphylococcus epidermidis RP62A |
title_short |
Stress conditions in the host induce persister cells and influence biofilm formation by Staphylococcus epidermidis RP62A |
title_full |
Stress conditions in the host induce persister cells and influence biofilm formation by Staphylococcus epidermidis RP62A |
title_fullStr |
Stress conditions in the host induce persister cells and influence biofilm formation by Staphylococcus epidermidis RP62A |
title_full_unstemmed |
Stress conditions in the host induce persister cells and influence biofilm formation by Staphylococcus epidermidis RP62A |
title_sort |
stress conditions in the host induce persister cells and influence biofilm formation by staphylococcus epidermidis rp62a |
description |
Abstract INTRODUCTION: Studies have demonstrated that pathogens react to the harsh conditions in human tissues by inducing mechanisms that promote survival. METHODS: Persistence and biofilm-forming ability were evaluated during stress conditions that mimic those in the host. RESULTS: Carbon-source availability had a positive effect on Staphylococcus epidermidis RP62A adhesion during hypoxia, accompanied by a decrease in pH. In contrast, iron limitation led to decreased surface-adherent biomass, accompanied by an increase medium acidification and lactate levels. Interestingly, iron starvation and hypoxia induced persister cells in planktonic culture. CONCLUSIONS: These findings highlight the role of host stress in the virulence of S. epidermidis. |
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Sociedade Brasileira de Medicina Tropical - SBMT |
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2019 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822019000100608 |
work_keys_str_mv |
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