Genes that encodes NAGT, MIF1 and MIF2 are not virulence factors for kala-azar caused by Leishmania infantum
IntroductionKala-azar is a disease resulting from infection by Leishmania donovani and Leishmania infantum. Most patients with the disease exhibit prolonged fever, wasting, anemia and hepatosplenomegaly without complications. However, some patients develop severe disease with hemorrhagic manifestations, bacterial infections, jaundice, and edema dyspnea, among other symptoms, followed by death. Among the parasite molecules that might influence the disease severity are the macrophage migration inhibitory factor-like proteins (MIF1 and MIF2) and N-acetylglucosamine-1-phosphotransferase (NAGT), which act in the first step of protein N-glycosylation. This study aimed to determine whether MIF1, MIF2 and NAGT are virulence factors for severe kala-azar.MethodsTo determine the parasite genotype in kala-azar patients from Northeastern Brazil, we sequenced the NAGT genes of L. infantum from 68 patients as well as the MIF1 and MIF2 genes from 76 different subjects with diverse clinical manifestations. After polymerase chain reaction (PCR), the fragments were sequenced, followed by polymorphism identification.ResultsThe nucleotide sequencing of the 144 amplicons revealed the absence of genetic variability of the NAGT, MIF1 and MIF2 genes between the isolates. The conservation of these genes suggests that the clinical variability of kala-azar does not depend upon these genes. Additionally, this conservation suggests that these genes may be critical for parasite survival.ConclusionsNAGT, MIF1 and MIF2 do not alter the severity of kala-azar. NAGT, MIF1 and MIF2 are highly conserved among different isolates of identical species and exhibit potential for use in phylogenetic inferences or molecular diagnosis.
Main Authors: | Aguiar,Bruno Guedes Alcoforado, Coelho,Daniela Lemos, Costa,Dorcas Lamounier, Drumond,Betânia Paiva, Coelho,Luiz Felipe Leomil, Figueiredo,Lívio Carvalho, Zacarias,Danielle Alves, Silva,Jailthon Carlos da, Alonso,Diego Peres, Ribolla,Paulo Eduardo Martins, Ishikawa,Edna Aoba Yassui, Gaído,Samara Belchior, Costa,Carlos Henrique Nery |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Sociedade Brasileira de Medicina Tropical - SBMT
2014
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822014000500593 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Study of the relationship between human MIF level, MIF-794CATT5-8 microsatellite polymorphism, and susceptibility of tuberculosis in southwest China
by: Li,Yanlin, et al.
Published: (2012) -
Avaliação funcional de pessoas com lesão medular: utilização da escala de independência funcional - MIF
by: Silva,Gelson Aguiar da, et al.
Published: (2012) -
Rényi entropy and cauchy-schwartz mutual information applied to mifs-u variable selection algorithm: a comparative study
by: Gonçalves,Leonardo Barroso, et al.
Published: (2011) -
Dysproteinaemia in the differential diagnosis of kala-azar
by: Moriearty,Pamela Lane, et al.
Published: (1981) -
Recurrent kala-azar: report of two cured cases after total splenectomy
by: Reinaldo,Luis Gustavo Cavalcante, et al.
Published: (2020)