Genes that encodes NAGT, MIF1 and MIF2 are not virulence factors for kala-azar caused by Leishmania infantum
IntroductionKala-azar is a disease resulting from infection by Leishmania donovani and Leishmania infantum. Most patients with the disease exhibit prolonged fever, wasting, anemia and hepatosplenomegaly without complications. However, some patients develop severe disease with hemorrhagic manifestations, bacterial infections, jaundice, and edema dyspnea, among other symptoms, followed by death. Among the parasite molecules that might influence the disease severity are the macrophage migration inhibitory factor-like proteins (MIF1 and MIF2) and N-acetylglucosamine-1-phosphotransferase (NAGT), which act in the first step of protein N-glycosylation. This study aimed to determine whether MIF1, MIF2 and NAGT are virulence factors for severe kala-azar.MethodsTo determine the parasite genotype in kala-azar patients from Northeastern Brazil, we sequenced the NAGT genes of L. infantum from 68 patients as well as the MIF1 and MIF2 genes from 76 different subjects with diverse clinical manifestations. After polymerase chain reaction (PCR), the fragments were sequenced, followed by polymorphism identification.ResultsThe nucleotide sequencing of the 144 amplicons revealed the absence of genetic variability of the NAGT, MIF1 and MIF2 genes between the isolates. The conservation of these genes suggests that the clinical variability of kala-azar does not depend upon these genes. Additionally, this conservation suggests that these genes may be critical for parasite survival.ConclusionsNAGT, MIF1 and MIF2 do not alter the severity of kala-azar. NAGT, MIF1 and MIF2 are highly conserved among different isolates of identical species and exhibit potential for use in phylogenetic inferences or molecular diagnosis.
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Sociedade Brasileira de Medicina Tropical - SBMT
2014
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oai:scielo:S0037-868220140005005932015-10-26Genes that encodes NAGT, MIF1 and MIF2 are not virulence factors for kala-azar caused by Leishmania infantumAguiar,Bruno Guedes AlcoforadoCoelho,Daniela LemosCosta,Dorcas LamounierDrumond,Betânia PaivaCoelho,Luiz Felipe LeomilFigueiredo,Lívio CarvalhoZacarias,Danielle AlvesSilva,Jailthon Carlos daAlonso,Diego PeresRibolla,Paulo Eduardo MartinsIshikawa,Edna Aoba YassuiGaído,Samara BelchiorCosta,Carlos Henrique Nery Genetic diversity Kala-azar Visceral leishmaniasis Macrophage inhibition factor Tropical diseases Single nucleotide polymorphism IntroductionKala-azar is a disease resulting from infection by Leishmania donovani and Leishmania infantum. Most patients with the disease exhibit prolonged fever, wasting, anemia and hepatosplenomegaly without complications. However, some patients develop severe disease with hemorrhagic manifestations, bacterial infections, jaundice, and edema dyspnea, among other symptoms, followed by death. Among the parasite molecules that might influence the disease severity are the macrophage migration inhibitory factor-like proteins (MIF1 and MIF2) and N-acetylglucosamine-1-phosphotransferase (NAGT), which act in the first step of protein N-glycosylation. This study aimed to determine whether MIF1, MIF2 and NAGT are virulence factors for severe kala-azar.MethodsTo determine the parasite genotype in kala-azar patients from Northeastern Brazil, we sequenced the NAGT genes of L. infantum from 68 patients as well as the MIF1 and MIF2 genes from 76 different subjects with diverse clinical manifestations. After polymerase chain reaction (PCR), the fragments were sequenced, followed by polymorphism identification.ResultsThe nucleotide sequencing of the 144 amplicons revealed the absence of genetic variability of the NAGT, MIF1 and MIF2 genes between the isolates. The conservation of these genes suggests that the clinical variability of kala-azar does not depend upon these genes. Additionally, this conservation suggests that these genes may be critical for parasite survival.ConclusionsNAGT, MIF1 and MIF2 do not alter the severity of kala-azar. NAGT, MIF1 and MIF2 are highly conserved among different isolates of identical species and exhibit potential for use in phylogenetic inferences or molecular diagnosis.info:eu-repo/semantics/openAccessSociedade Brasileira de Medicina Tropical - SBMTRevista da Sociedade Brasileira de Medicina Tropical v.47 n.5 20142014-10-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822014000500593en10.1590/0037-8682-0183-2014 |
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Aguiar,Bruno Guedes Alcoforado Coelho,Daniela Lemos Costa,Dorcas Lamounier Drumond,Betânia Paiva Coelho,Luiz Felipe Leomil Figueiredo,Lívio Carvalho Zacarias,Danielle Alves Silva,Jailthon Carlos da Alonso,Diego Peres Ribolla,Paulo Eduardo Martins Ishikawa,Edna Aoba Yassui Gaído,Samara Belchior Costa,Carlos Henrique Nery |
spellingShingle |
Aguiar,Bruno Guedes Alcoforado Coelho,Daniela Lemos Costa,Dorcas Lamounier Drumond,Betânia Paiva Coelho,Luiz Felipe Leomil Figueiredo,Lívio Carvalho Zacarias,Danielle Alves Silva,Jailthon Carlos da Alonso,Diego Peres Ribolla,Paulo Eduardo Martins Ishikawa,Edna Aoba Yassui Gaído,Samara Belchior Costa,Carlos Henrique Nery Genes that encodes NAGT, MIF1 and MIF2 are not virulence factors for kala-azar caused by Leishmania infantum |
author_facet |
Aguiar,Bruno Guedes Alcoforado Coelho,Daniela Lemos Costa,Dorcas Lamounier Drumond,Betânia Paiva Coelho,Luiz Felipe Leomil Figueiredo,Lívio Carvalho Zacarias,Danielle Alves Silva,Jailthon Carlos da Alonso,Diego Peres Ribolla,Paulo Eduardo Martins Ishikawa,Edna Aoba Yassui Gaído,Samara Belchior Costa,Carlos Henrique Nery |
author_sort |
Aguiar,Bruno Guedes Alcoforado |
title |
Genes that encodes NAGT, MIF1 and MIF2 are not virulence factors for kala-azar caused by Leishmania infantum |
title_short |
Genes that encodes NAGT, MIF1 and MIF2 are not virulence factors for kala-azar caused by Leishmania infantum |
title_full |
Genes that encodes NAGT, MIF1 and MIF2 are not virulence factors for kala-azar caused by Leishmania infantum |
title_fullStr |
Genes that encodes NAGT, MIF1 and MIF2 are not virulence factors for kala-azar caused by Leishmania infantum |
title_full_unstemmed |
Genes that encodes NAGT, MIF1 and MIF2 are not virulence factors for kala-azar caused by Leishmania infantum |
title_sort |
genes that encodes nagt, mif1 and mif2 are not virulence factors for kala-azar caused by leishmania infantum |
description |
IntroductionKala-azar is a disease resulting from infection by Leishmania donovani and Leishmania infantum. Most patients with the disease exhibit prolonged fever, wasting, anemia and hepatosplenomegaly without complications. However, some patients develop severe disease with hemorrhagic manifestations, bacterial infections, jaundice, and edema dyspnea, among other symptoms, followed by death. Among the parasite molecules that might influence the disease severity are the macrophage migration inhibitory factor-like proteins (MIF1 and MIF2) and N-acetylglucosamine-1-phosphotransferase (NAGT), which act in the first step of protein N-glycosylation. This study aimed to determine whether MIF1, MIF2 and NAGT are virulence factors for severe kala-azar.MethodsTo determine the parasite genotype in kala-azar patients from Northeastern Brazil, we sequenced the NAGT genes of L. infantum from 68 patients as well as the MIF1 and MIF2 genes from 76 different subjects with diverse clinical manifestations. After polymerase chain reaction (PCR), the fragments were sequenced, followed by polymorphism identification.ResultsThe nucleotide sequencing of the 144 amplicons revealed the absence of genetic variability of the NAGT, MIF1 and MIF2 genes between the isolates. The conservation of these genes suggests that the clinical variability of kala-azar does not depend upon these genes. Additionally, this conservation suggests that these genes may be critical for parasite survival.ConclusionsNAGT, MIF1 and MIF2 do not alter the severity of kala-azar. NAGT, MIF1 and MIF2 are highly conserved among different isolates of identical species and exhibit potential for use in phylogenetic inferences or molecular diagnosis. |
publisher |
Sociedade Brasileira de Medicina Tropical - SBMT |
publishDate |
2014 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822014000500593 |
work_keys_str_mv |
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