Susceptibility of Biomphalaria tenagophila and Biomphalaria straminea to Schistosoma mansoni infection detected by low stringency polymerase chain reaction

Susceptibility of Biomphalaria tenagophila and Biomphalaria straminea to Schistosoma mansoni infection detected by low stringency polymerase chain reaction

In order to determine Schistosoma mansoni infection rates in Biomphalaria tenagophila and B. straminea, low stringency polymerase chain reaction (LS-PCR) technique was used as a complementary method to light exposure technique. LS-PCR has already been standardized in our laboratory to detect the trematode DNA in B. glabrata. Higher S. mansoni infection rates were detected using conventional method and LS-PCR. The parasite DNA profile was detected in both species after 7-day exposure to miracidia, using LS-PCR. This technique enables early detection of schistosomiasis transmission focuses, in endemic areas, before the beginning of cercariae shedding.

Saved in:
Bibliographic Details
Main Authors: JANNOTTI-PASSOS,Liana Konovaloff, SOUZA,Cecilia Pereira de
Format: Digital revista
Language:English
Published: Instituto de Medicina Tropical de São Paulo 2000
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652000000500010
Tags: Add Tag
No Tags, Be the first to tag this record!
id oai:scielo:S0036-46652000000500010
record_format ojs
spelling oai:scielo:S0036-466520000005000102000-10-25Susceptibility of Biomphalaria tenagophila and Biomphalaria straminea to Schistosoma mansoni infection detected by low stringency polymerase chain reactionJANNOTTI-PASSOS,Liana KonovaloffSOUZA,Cecilia Pereira de Schistosoma mansoni Biomphalaria tenagophila Biomphalaria straminea Susceptibility LS-PCR technique In order to determine Schistosoma mansoni infection rates in Biomphalaria tenagophila and B. straminea, low stringency polymerase chain reaction (LS-PCR) technique was used as a complementary method to light exposure technique. LS-PCR has already been standardized in our laboratory to detect the trematode DNA in B. glabrata. Higher S. mansoni infection rates were detected using conventional method and LS-PCR. The parasite DNA profile was detected in both species after 7-day exposure to miracidia, using LS-PCR. This technique enables early detection of schistosomiasis transmission focuses, in endemic areas, before the beginning of cercariae shedding.info:eu-repo/semantics/openAccessInstituto de Medicina Tropical de São PauloRevista do Instituto de Medicina Tropical de São Paulo v.42 n.5 20002000-10-01info:eu-repo/semantics/othertext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652000000500010en10.1590/S0036-46652000000500010
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author JANNOTTI-PASSOS,Liana Konovaloff
SOUZA,Cecilia Pereira de
spellingShingle JANNOTTI-PASSOS,Liana Konovaloff
SOUZA,Cecilia Pereira de
Susceptibility of Biomphalaria tenagophila and Biomphalaria straminea to Schistosoma mansoni infection detected by low stringency polymerase chain reaction
author_facet JANNOTTI-PASSOS,Liana Konovaloff
SOUZA,Cecilia Pereira de
author_sort JANNOTTI-PASSOS,Liana Konovaloff
title Susceptibility of Biomphalaria tenagophila and Biomphalaria straminea to Schistosoma mansoni infection detected by low stringency polymerase chain reaction
title_short Susceptibility of Biomphalaria tenagophila and Biomphalaria straminea to Schistosoma mansoni infection detected by low stringency polymerase chain reaction
title_full Susceptibility of Biomphalaria tenagophila and Biomphalaria straminea to Schistosoma mansoni infection detected by low stringency polymerase chain reaction
title_fullStr Susceptibility of Biomphalaria tenagophila and Biomphalaria straminea to Schistosoma mansoni infection detected by low stringency polymerase chain reaction
title_full_unstemmed Susceptibility of Biomphalaria tenagophila and Biomphalaria straminea to Schistosoma mansoni infection detected by low stringency polymerase chain reaction
title_sort susceptibility of biomphalaria tenagophila and biomphalaria straminea to schistosoma mansoni infection detected by low stringency polymerase chain reaction
description In order to determine Schistosoma mansoni infection rates in Biomphalaria tenagophila and B. straminea, low stringency polymerase chain reaction (LS-PCR) technique was used as a complementary method to light exposure technique. LS-PCR has already been standardized in our laboratory to detect the trematode DNA in B. glabrata. Higher S. mansoni infection rates were detected using conventional method and LS-PCR. The parasite DNA profile was detected in both species after 7-day exposure to miracidia, using LS-PCR. This technique enables early detection of schistosomiasis transmission focuses, in endemic areas, before the beginning of cercariae shedding.
publisher Instituto de Medicina Tropical de São Paulo
publishDate 2000
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652000000500010
work_keys_str_mv AT jannottipassoslianakonovaloff susceptibilityofbiomphalariatenagophilaandbiomphalariastramineatoschistosomamansoniinfectiondetectedbylowstringencypolymerasechainreaction
AT souzaceciliapereirade susceptibilityofbiomphalariatenagophilaandbiomphalariastramineatoschistosomamansoniinfectiondetectedbylowstringencypolymerasechainreaction
_version_ 1756379716959338496

Similar Items