Production of monoclonal antibodies anti-Taenia crassiceps cysticerci with cross-reactivity with Taenia solium antigens

Production of monoclonal antibodies anti-Taenia crassiceps cysticerci with cross-reactivity with Taenia solium antigens

We describe the production of the potential monoclonal antibodies (MoAbs) using BALB/c mice immunized with vesicular fluid (VF)-Tcra (T. crassiceps) antigen. Immune sera presented anti-VF-Tcra (<20kD) IgG and IgM antibodies with cross-reactivity with T. solium (Tso) antigen (8-12, 14, and 18 kD). After cell fusion, we selected 33 anti-Tcra and anti-Tso reactive IgM-clones and 53 anti-Tcra specific IgG-clones, 5 of them also recognizing Tso antigens. Two clones identified the 8-14 and 18kD peptides of VF-Tcra.

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Main Authors: ESPÍNDOLA,Noeli M., DE GASPARI,Elizabeth N., NAKAMURA,Paulo M., VAZ,Adelaide J.
Format: Digital revista
Language:English
Published: Instituto de Medicina Tropical de São Paulo 2000
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652000000300013
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spelling oai:scielo:S0036-466520000003000132000-07-24Production of monoclonal antibodies anti-Taenia crassiceps cysticerci with cross-reactivity with Taenia solium antigensESPÍNDOLA,Noeli M.DE GASPARI,Elizabeth N.NAKAMURA,Paulo M.VAZ,Adelaide J. Taenia solium Taenia crassiceps Monoclonal antibody Cysticercosis We describe the production of the potential monoclonal antibodies (MoAbs) using BALB/c mice immunized with vesicular fluid (VF)-Tcra (T. crassiceps) antigen. Immune sera presented anti-VF-Tcra (<20kD) IgG and IgM antibodies with cross-reactivity with T. solium (Tso) antigen (8-12, 14, and 18 kD). After cell fusion, we selected 33 anti-Tcra and anti-Tso reactive IgM-clones and 53 anti-Tcra specific IgG-clones, 5 of them also recognizing Tso antigens. Two clones identified the 8-14 and 18kD peptides of VF-Tcra.info:eu-repo/semantics/openAccessInstituto de Medicina Tropical de São PauloRevista do Instituto de Medicina Tropical de São Paulo v.42 n.3 20002000-06-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652000000300013en10.1590/S0036-46652000000300013
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author ESPÍNDOLA,Noeli M.
DE GASPARI,Elizabeth N.
NAKAMURA,Paulo M.
VAZ,Adelaide J.
spellingShingle ESPÍNDOLA,Noeli M.
DE GASPARI,Elizabeth N.
NAKAMURA,Paulo M.
VAZ,Adelaide J.
Production of monoclonal antibodies anti-Taenia crassiceps cysticerci with cross-reactivity with Taenia solium antigens
author_facet ESPÍNDOLA,Noeli M.
DE GASPARI,Elizabeth N.
NAKAMURA,Paulo M.
VAZ,Adelaide J.
author_sort ESPÍNDOLA,Noeli M.
title Production of monoclonal antibodies anti-Taenia crassiceps cysticerci with cross-reactivity with Taenia solium antigens
title_short Production of monoclonal antibodies anti-Taenia crassiceps cysticerci with cross-reactivity with Taenia solium antigens
title_full Production of monoclonal antibodies anti-Taenia crassiceps cysticerci with cross-reactivity with Taenia solium antigens
title_fullStr Production of monoclonal antibodies anti-Taenia crassiceps cysticerci with cross-reactivity with Taenia solium antigens
title_full_unstemmed Production of monoclonal antibodies anti-Taenia crassiceps cysticerci with cross-reactivity with Taenia solium antigens
title_sort production of monoclonal antibodies anti-taenia crassiceps cysticerci with cross-reactivity with taenia solium antigens
description We describe the production of the potential monoclonal antibodies (MoAbs) using BALB/c mice immunized with vesicular fluid (VF)-Tcra (T. crassiceps) antigen. Immune sera presented anti-VF-Tcra (<20kD) IgG and IgM antibodies with cross-reactivity with T. solium (Tso) antigen (8-12, 14, and 18 kD). After cell fusion, we selected 33 anti-Tcra and anti-Tso reactive IgM-clones and 53 anti-Tcra specific IgG-clones, 5 of them also recognizing Tso antigens. Two clones identified the 8-14 and 18kD peptides of VF-Tcra.
publisher Instituto de Medicina Tropical de São Paulo
publishDate 2000
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652000000300013
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AT nakamurapaulom productionofmonoclonalantibodiesantitaeniacrassicepscysticerciwithcrossreactivitywithtaeniasoliumantigens
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