ELISA test for the diagnosis of cysticercosis in pigs using antigens of Taenia solium and Taenia crassiceps cysticerci
In the present study ELISA was standardized for the diagnosis of swine cysticercosis based on necropsy parameters and confirmed positive and negative control sera. Serum samples from pigs with other infections were also assayed to determine possible cross-reactions. Four antigens were assayed: from Taenia crassiceps vesicular fluid (VF-Tcra) and crude larvae extract (T-Tcra), and from Taenia solium extracts of scolex (S-Ts) and of larvae (T-Ts). A checkerboard evaluation of antigen, serum and conjugate dilutions, as well as the use of Tween-20 and skim cow milk in wash and blocking solution had a marked effect on improving ELISA performance. All the antigens showed a good performance, but VF-Tcra was the best, with 96.0% and 80.0% sensitivities for cut-offs respectively at 2sd and 3sd, and corresponding specificities of 97.5% and 100.0%. Cross-reactivity was observed only with hydatidosis and ascaridiosis. In view of the high performance observed, the ELISA test should be recommended for the diagnosis of cysticercosis in suspected swine in slaughterhouses and for the screening of cysticercosis in swine production. These results will support integrated measures of cysticercosis control throughout the chain of swine production, effectively contributing to public health.
Principais autores: | PINTO,Paulo Sérgio de Arruda, VAZ,Adelaide José, GERMANO,Pedro Manuel Leal, NAKAMURA,Paulo Mutuko |
---|---|
Formato: | Digital revista |
Idioma: | English |
Publicado em: |
Instituto de Medicina Tropical de São Paulo
2000
|
Acesso em linha: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652000000200003 |
Tags: |
Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!
|
Registros relacionados
-
Immunoblot analysis using antigen from Taenia crassiceps cysticerci in the diagnosis of swine cysticercosis
por: de Arruda Pinto,Paulo Sérgio, et al.
Publicado em: (2001) -
Production of monoclonal antibodies anti-Taenia crassiceps cysticerci with cross-reactivity with Taenia solium antigens
por: ESPÍNDOLA,Noeli M., et al.
Publicado em: (2000) -
Characterization of Taenia crassiceps cysticerci and their application to diagnosis of the human cysticercosis
por: Espíndola,Noeli Maria
Publicado em: (2004) -
Evaluation of Taenia solium and Taenia crassiceps cysticercal antigens for immunodiagnosis of neurocysticercosis using ELISA on cerebrospinal fluid samples
por: Suzuki,Lisandra Akemi, et al.
Publicado em: (2007) -
Experimental encephalitis caused by Taenia crassiceps cysticerci in mice
por: Matos-Silva,Hidelberto, et al.
Publicado em: (2012)