Preliminar characterization of a new virus isolated from the spinal fluid of patients with meningitis in São Paulo, Brazil
A total of 138 patients with the age of 4 months to 57 years were attended in different hospitals of São Paulo State with aseptic meningitis. A probable new agent was isolated from the cerebrospinal fluid of 35 of 53 specimens examined. Replication of the agent with similar characteristics was detected by CPE produced in the MDCK cell line. Virus-like particles measuring about 40 nm in diameter were observed by negative staining electron microscopy. No hemaglutinating activity was detected at pH 7.2 by using either human, guinea pig, chicken and at pH ranged 6.0 - 7.2 with goose red blood cells. The agent was not pathogenic to newborn or adult mice. Virus infectivity as measured by CPE was sensitive to chloroform and not inhibited by BuDR, suggesting that agent is an enveloped virus with RNA genome.
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Instituto de Medicina Tropical de São Paulo
1990
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oai:scielo:S0036-466519900003000032006-09-13Preliminar characterization of a new virus isolated from the spinal fluid of patients with meningitis in São Paulo, BrazilTakimoto,SuekoTanaka,HatuneGomes,Luis F. SallesWaldman,Eliseu A.Perreira,Helio G.Paiva,Teresinha M.Coimbra,Teresinha L.Ueda,MarliMeles,Heloísa H. G. Aseptic meningitis Aetiologic agent Characterization of meningitis associated virus A total of 138 patients with the age of 4 months to 57 years were attended in different hospitals of São Paulo State with aseptic meningitis. A probable new agent was isolated from the cerebrospinal fluid of 35 of 53 specimens examined. Replication of the agent with similar characteristics was detected by CPE produced in the MDCK cell line. Virus-like particles measuring about 40 nm in diameter were observed by negative staining electron microscopy. No hemaglutinating activity was detected at pH 7.2 by using either human, guinea pig, chicken and at pH ranged 6.0 - 7.2 with goose red blood cells. The agent was not pathogenic to newborn or adult mice. Virus infectivity as measured by CPE was sensitive to chloroform and not inhibited by BuDR, suggesting that agent is an enveloped virus with RNA genome.info:eu-repo/semantics/openAccessInstituto de Medicina Tropical de São PauloRevista do Instituto de Medicina Tropical de São Paulo v.32 n.3 19901990-06-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651990000300003en10.1590/S0036-46651990000300003 |
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revista |
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| author |
Takimoto,Sueko Tanaka,Hatune Gomes,Luis F. Salles Waldman,Eliseu A. Perreira,Helio G. Paiva,Teresinha M. Coimbra,Teresinha L. Ueda,Marli Meles,Heloísa H. G. |
| spellingShingle |
Takimoto,Sueko Tanaka,Hatune Gomes,Luis F. Salles Waldman,Eliseu A. Perreira,Helio G. Paiva,Teresinha M. Coimbra,Teresinha L. Ueda,Marli Meles,Heloísa H. G. Preliminar characterization of a new virus isolated from the spinal fluid of patients with meningitis in São Paulo, Brazil |
| author_facet |
Takimoto,Sueko Tanaka,Hatune Gomes,Luis F. Salles Waldman,Eliseu A. Perreira,Helio G. Paiva,Teresinha M. Coimbra,Teresinha L. Ueda,Marli Meles,Heloísa H. G. |
| author_sort |
Takimoto,Sueko |
| title |
Preliminar characterization of a new virus isolated from the spinal fluid of patients with meningitis in São Paulo, Brazil |
| title_short |
Preliminar characterization of a new virus isolated from the spinal fluid of patients with meningitis in São Paulo, Brazil |
| title_full |
Preliminar characterization of a new virus isolated from the spinal fluid of patients with meningitis in São Paulo, Brazil |
| title_fullStr |
Preliminar characterization of a new virus isolated from the spinal fluid of patients with meningitis in São Paulo, Brazil |
| title_full_unstemmed |
Preliminar characterization of a new virus isolated from the spinal fluid of patients with meningitis in São Paulo, Brazil |
| title_sort |
preliminar characterization of a new virus isolated from the spinal fluid of patients with meningitis in são paulo, brazil |
| description |
A total of 138 patients with the age of 4 months to 57 years were attended in different hospitals of São Paulo State with aseptic meningitis. A probable new agent was isolated from the cerebrospinal fluid of 35 of 53 specimens examined. Replication of the agent with similar characteristics was detected by CPE produced in the MDCK cell line. Virus-like particles measuring about 40 nm in diameter were observed by negative staining electron microscopy. No hemaglutinating activity was detected at pH 7.2 by using either human, guinea pig, chicken and at pH ranged 6.0 - 7.2 with goose red blood cells. The agent was not pathogenic to newborn or adult mice. Virus infectivity as measured by CPE was sensitive to chloroform and not inhibited by BuDR, suggesting that agent is an enveloped virus with RNA genome. |
| publisher |
Instituto de Medicina Tropical de São Paulo |
| publishDate |
1990 |
| url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651990000300003 |
| work_keys_str_mv |
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