Identification of 'Ubá' mango tree zygotic and nucellar seedlings using ISSR markers
Polyembryonic seeds are characterized by the development of over one embryo in the same seed, which can be zygotic and nucellar. The objective of this work was to identify the genetic origin, whether zygotic or nucellar, of seedlings of polyembryonic seeds of 'Ubá' mango tree using ISSR markers, and relating them with the vigor of the seedlings. Thus, mangos were harvested in Visconde do Rio Branco (accession 102) and Ubá (accessions 112, 138, 152 and 159), whose seeds were germinated in plastic trays filled with washed sand. Fifty days after sowing, seedlings from five seeds of each one of the accessions 102, 112, 138, 159 and from 10 seeds of the accession 152, were analyzed. These sseedlings were characterized and evaluated for plant height, stem circumference and mass of fresh aerial part and the most vigorous seedling was the one displaying at least two of these traits higher than the other seedlings from seed. Leaves were collected for genomic DNA extraction, which was amplified using seven ISSR primers previously selected based on the amplification profile and considering the number and resolution of fragments. Zygotic seedlings were found in 18 seeds, which were the most vigorous in six seeds. The results evidenced the existence of genetic variability in orchards using seedlings grown from seeds, because the farmer usually uses the most vigorous ones, assuming that this is of nucellar origin. These results also indicate that the most vigorous seedling are not always nucellar, inasmuch as of 20% of the total seeds evaluated, the zygotic seedling was the most vigorous.
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Universidade Federal de Viçosa
2014
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oai:scielo:S0034-737X20140005000012014-12-02Identification of 'Ubá' mango tree zygotic and nucellar seedlings using ISSR markersRocha,AlineSalomão,Tânia Maria FernandesSiqueira,Dalmo Lopes deCruz,Cosme DamiãoSalomão,Luiz Carlos Chamhum Mangifera indica polyembryony rootstock mass selection Polyembryonic seeds are characterized by the development of over one embryo in the same seed, which can be zygotic and nucellar. The objective of this work was to identify the genetic origin, whether zygotic or nucellar, of seedlings of polyembryonic seeds of 'Ubá' mango tree using ISSR markers, and relating them with the vigor of the seedlings. Thus, mangos were harvested in Visconde do Rio Branco (accession 102) and Ubá (accessions 112, 138, 152 and 159), whose seeds were germinated in plastic trays filled with washed sand. Fifty days after sowing, seedlings from five seeds of each one of the accessions 102, 112, 138, 159 and from 10 seeds of the accession 152, were analyzed. These sseedlings were characterized and evaluated for plant height, stem circumference and mass of fresh aerial part and the most vigorous seedling was the one displaying at least two of these traits higher than the other seedlings from seed. Leaves were collected for genomic DNA extraction, which was amplified using seven ISSR primers previously selected based on the amplification profile and considering the number and resolution of fragments. Zygotic seedlings were found in 18 seeds, which were the most vigorous in six seeds. The results evidenced the existence of genetic variability in orchards using seedlings grown from seeds, because the farmer usually uses the most vigorous ones, assuming that this is of nucellar origin. These results also indicate that the most vigorous seedling are not always nucellar, inasmuch as of 20% of the total seeds evaluated, the zygotic seedling was the most vigorous.info:eu-repo/semantics/openAccessUniversidade Federal de ViçosaRevista Ceres v.61 n.5 20142014-10-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2014000500001en10.1590/0034-737X201461040001 |
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Rocha,Aline Salomão,Tânia Maria Fernandes Siqueira,Dalmo Lopes de Cruz,Cosme Damião Salomão,Luiz Carlos Chamhum |
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Rocha,Aline Salomão,Tânia Maria Fernandes Siqueira,Dalmo Lopes de Cruz,Cosme Damião Salomão,Luiz Carlos Chamhum Identification of 'Ubá' mango tree zygotic and nucellar seedlings using ISSR markers |
author_facet |
Rocha,Aline Salomão,Tânia Maria Fernandes Siqueira,Dalmo Lopes de Cruz,Cosme Damião Salomão,Luiz Carlos Chamhum |
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Rocha,Aline |
title |
Identification of 'Ubá' mango tree zygotic and nucellar seedlings using ISSR markers |
title_short |
Identification of 'Ubá' mango tree zygotic and nucellar seedlings using ISSR markers |
title_full |
Identification of 'Ubá' mango tree zygotic and nucellar seedlings using ISSR markers |
title_fullStr |
Identification of 'Ubá' mango tree zygotic and nucellar seedlings using ISSR markers |
title_full_unstemmed |
Identification of 'Ubá' mango tree zygotic and nucellar seedlings using ISSR markers |
title_sort |
identification of 'ubá' mango tree zygotic and nucellar seedlings using issr markers |
description |
Polyembryonic seeds are characterized by the development of over one embryo in the same seed, which can be zygotic and nucellar. The objective of this work was to identify the genetic origin, whether zygotic or nucellar, of seedlings of polyembryonic seeds of 'Ubá' mango tree using ISSR markers, and relating them with the vigor of the seedlings. Thus, mangos were harvested in Visconde do Rio Branco (accession 102) and Ubá (accessions 112, 138, 152 and 159), whose seeds were germinated in plastic trays filled with washed sand. Fifty days after sowing, seedlings from five seeds of each one of the accessions 102, 112, 138, 159 and from 10 seeds of the accession 152, were analyzed. These sseedlings were characterized and evaluated for plant height, stem circumference and mass of fresh aerial part and the most vigorous seedling was the one displaying at least two of these traits higher than the other seedlings from seed. Leaves were collected for genomic DNA extraction, which was amplified using seven ISSR primers previously selected based on the amplification profile and considering the number and resolution of fragments. Zygotic seedlings were found in 18 seeds, which were the most vigorous in six seeds. The results evidenced the existence of genetic variability in orchards using seedlings grown from seeds, because the farmer usually uses the most vigorous ones, assuming that this is of nucellar origin. These results also indicate that the most vigorous seedling are not always nucellar, inasmuch as of 20% of the total seeds evaluated, the zygotic seedling was the most vigorous. |
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Universidade Federal de Viçosa |
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2014 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2014000500001 |
work_keys_str_mv |
AT rochaaline identificationofubamangotreezygoticandnucellarseedlingsusingissrmarkers AT salomaotaniamariafernandes identificationofubamangotreezygoticandnucellarseedlingsusingissrmarkers AT siqueiradalmolopesde identificationofubamangotreezygoticandnucellarseedlingsusingissrmarkers AT cruzcosmedamiao identificationofubamangotreezygoticandnucellarseedlingsusingissrmarkers AT salomaoluizcarloschamhum identificationofubamangotreezygoticandnucellarseedlingsusingissrmarkers |
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1756377885285810176 |