In vitro isolation of Ehrlichia ruminantium from ovine blood into Ixodes scapularis (IDE8) cell cultures
Four stocks of Ehrlichia ruminantium (Welgevonden, Ball3, Nonile and Blaauwkrans), the causative agent of heartwater in domestic ruminants, were isolated into Ixodes scapularis (IDE8) tick cells using the leukocyte fraction of the blood of infected sheep. Organisms of two of the E. ruminantium stocks (Welgevonden and Blaauwkrans) propagated in IDE8 cells were also successfully used to infect bovine endothelial cells. All stocks were successfully propagated in IDE8 cells using Dulbecco's modified Eagle's medium nutrient mixture Ham F-12 containing 10 % foetal bovine serum (FBS). The technique should be included in any attempt to isolate uncharacterized E. ruminantium stocks.
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ARC-Onderstepoort Veterinary Institute - Agricultural Research Council
2008
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oai:scielo:S0030-246520080001000042015-03-26In vitro isolation of Ehrlichia ruminantium from ovine blood into Ixodes scapularis (IDE8) cell culturesZweygarth,E.Josemans,A.I.Steyn,H.C. Ehrlichia ruminantium heartwater in vitro isolation tick cell line Four stocks of Ehrlichia ruminantium (Welgevonden, Ball3, Nonile and Blaauwkrans), the causative agent of heartwater in domestic ruminants, were isolated into Ixodes scapularis (IDE8) tick cells using the leukocyte fraction of the blood of infected sheep. Organisms of two of the E. ruminantium stocks (Welgevonden and Blaauwkrans) propagated in IDE8 cells were also successfully used to infect bovine endothelial cells. All stocks were successfully propagated in IDE8 cells using Dulbecco's modified Eagle's medium nutrient mixture Ham F-12 containing 10 % foetal bovine serum (FBS). The technique should be included in any attempt to isolate uncharacterized E. ruminantium stocks.ARC-Onderstepoort Veterinary Institute - Agricultural Research CouncilOnderstepoort Journal of Veterinary Research v.75 n.2 20082008-01-01journal articletext/htmlhttp://www.scielo.org.za/scielo.php?script=sci_arttext&pid=S0030-24652008000100004en |
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Zweygarth,E. Josemans,A.I. Steyn,H.C. |
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Zweygarth,E. Josemans,A.I. Steyn,H.C. In vitro isolation of Ehrlichia ruminantium from ovine blood into Ixodes scapularis (IDE8) cell cultures |
| author_facet |
Zweygarth,E. Josemans,A.I. Steyn,H.C. |
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Zweygarth,E. |
| title |
In vitro isolation of Ehrlichia ruminantium from ovine blood into Ixodes scapularis (IDE8) cell cultures |
| title_short |
In vitro isolation of Ehrlichia ruminantium from ovine blood into Ixodes scapularis (IDE8) cell cultures |
| title_full |
In vitro isolation of Ehrlichia ruminantium from ovine blood into Ixodes scapularis (IDE8) cell cultures |
| title_fullStr |
In vitro isolation of Ehrlichia ruminantium from ovine blood into Ixodes scapularis (IDE8) cell cultures |
| title_full_unstemmed |
In vitro isolation of Ehrlichia ruminantium from ovine blood into Ixodes scapularis (IDE8) cell cultures |
| title_sort |
in vitro isolation of ehrlichia ruminantium from ovine blood into ixodes scapularis (ide8) cell cultures |
| description |
Four stocks of Ehrlichia ruminantium (Welgevonden, Ball3, Nonile and Blaauwkrans), the causative agent of heartwater in domestic ruminants, were isolated into Ixodes scapularis (IDE8) tick cells using the leukocyte fraction of the blood of infected sheep. Organisms of two of the E. ruminantium stocks (Welgevonden and Blaauwkrans) propagated in IDE8 cells were also successfully used to infect bovine endothelial cells. All stocks were successfully propagated in IDE8 cells using Dulbecco's modified Eagle's medium nutrient mixture Ham F-12 containing 10 % foetal bovine serum (FBS). The technique should be included in any attempt to isolate uncharacterized E. ruminantium stocks. |
| publisher |
ARC-Onderstepoort Veterinary Institute - Agricultural Research Council |
| publishDate |
2008 |
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http://www.scielo.org.za/scielo.php?script=sci_arttext&pid=S0030-24652008000100004 |
| work_keys_str_mv |
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1756004582448693248 |