Intestinal permeability assessed by 51Cr-EDTA in rats with CCl4 - induced cirrhosis
CONTEXT: The straight relationship between cirrhosis and impaired intestinal barrier has not been elucidated yet. OBJECTIVES: To verify 51Cr-EDTA-intestinal permeability in rats with CCl4-induced cirrhosis and controls. METHOD: Fifty male Wistar rats weighing 150-180 g were separated in three groups: 25 animals received CCl4 0.25 mL/kg with olive oil by gavage with 12 g/rat/day food restriction for 10 weeks (CCl4-induced cirrhosis); 12 received the same food restriction for 10 weeks (CCl4-non exposed). Other 13 rats received indomethacin 15 mg/kg by gavage as positive control of intestinal inflammation. RESULTS: The median (25-75 interquartile range) 51Cr-EDTA-IP values of cirrhotic and CCl4-non exposed rats were 0.90% (0.63-1.79) and 0.90% (0.60-1.52) respectively, without significant difference (P = 0.65). Animals from indomethacin group showed 51Cr-EDTA-IP, median 7.3% (5.1-14.7), significantly higher than cirrhotic and CCl4-non exposed rats (P<0.001). CONCLUSION: This study showed the lack of difference between 51Cr-EDTA-intestinal permeability in rats with and without cirrhosis. Further studies are necessary to better clarify the relationship between intestinal permeability and cirrhosis.
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Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia e Outras Especialidades - IBEPEGE.
2010
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oai:scielo:S0004-280320100002000142010-09-22Intestinal permeability assessed by 51Cr-EDTA in rats with CCl4 - induced cirrhosisRamos,Ana Regina L.Matte,UrsulaGoldani,Helena Ayako SuenoOliveira,Osmar L. M.Vieira,Sandra Maria GonçalvesSilveira,Themis Reverbel da Intestinal absorption Edetic acid Liver cirrhosis, experimental Rats CONTEXT: The straight relationship between cirrhosis and impaired intestinal barrier has not been elucidated yet. OBJECTIVES: To verify 51Cr-EDTA-intestinal permeability in rats with CCl4-induced cirrhosis and controls. METHOD: Fifty male Wistar rats weighing 150-180 g were separated in three groups: 25 animals received CCl4 0.25 mL/kg with olive oil by gavage with 12 g/rat/day food restriction for 10 weeks (CCl4-induced cirrhosis); 12 received the same food restriction for 10 weeks (CCl4-non exposed). Other 13 rats received indomethacin 15 mg/kg by gavage as positive control of intestinal inflammation. RESULTS: The median (25-75 interquartile range) 51Cr-EDTA-IP values of cirrhotic and CCl4-non exposed rats were 0.90% (0.63-1.79) and 0.90% (0.60-1.52) respectively, without significant difference (P = 0.65). Animals from indomethacin group showed 51Cr-EDTA-IP, median 7.3% (5.1-14.7), significantly higher than cirrhotic and CCl4-non exposed rats (P<0.001). CONCLUSION: This study showed the lack of difference between 51Cr-EDTA-intestinal permeability in rats with and without cirrhosis. Further studies are necessary to better clarify the relationship between intestinal permeability and cirrhosis.info:eu-repo/semantics/openAccessInstituto Brasileiro de Estudos e Pesquisas de Gastroenterologia e Outras Especialidades - IBEPEGE. Arquivos de Gastroenterologia v.47 n.2 20102010-06-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-28032010000200014en10.1590/S0004-28032010000200014 |
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Ramos,Ana Regina L. Matte,Ursula Goldani,Helena Ayako Sueno Oliveira,Osmar L. M. Vieira,Sandra Maria Gonçalves Silveira,Themis Reverbel da |
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Ramos,Ana Regina L. Matte,Ursula Goldani,Helena Ayako Sueno Oliveira,Osmar L. M. Vieira,Sandra Maria Gonçalves Silveira,Themis Reverbel da Intestinal permeability assessed by 51Cr-EDTA in rats with CCl4 - induced cirrhosis |
author_facet |
Ramos,Ana Regina L. Matte,Ursula Goldani,Helena Ayako Sueno Oliveira,Osmar L. M. Vieira,Sandra Maria Gonçalves Silveira,Themis Reverbel da |
author_sort |
Ramos,Ana Regina L. |
title |
Intestinal permeability assessed by 51Cr-EDTA in rats with CCl4 - induced cirrhosis |
title_short |
Intestinal permeability assessed by 51Cr-EDTA in rats with CCl4 - induced cirrhosis |
title_full |
Intestinal permeability assessed by 51Cr-EDTA in rats with CCl4 - induced cirrhosis |
title_fullStr |
Intestinal permeability assessed by 51Cr-EDTA in rats with CCl4 - induced cirrhosis |
title_full_unstemmed |
Intestinal permeability assessed by 51Cr-EDTA in rats with CCl4 - induced cirrhosis |
title_sort |
intestinal permeability assessed by 51cr-edta in rats with ccl4 - induced cirrhosis |
description |
CONTEXT: The straight relationship between cirrhosis and impaired intestinal barrier has not been elucidated yet. OBJECTIVES: To verify 51Cr-EDTA-intestinal permeability in rats with CCl4-induced cirrhosis and controls. METHOD: Fifty male Wistar rats weighing 150-180 g were separated in three groups: 25 animals received CCl4 0.25 mL/kg with olive oil by gavage with 12 g/rat/day food restriction for 10 weeks (CCl4-induced cirrhosis); 12 received the same food restriction for 10 weeks (CCl4-non exposed). Other 13 rats received indomethacin 15 mg/kg by gavage as positive control of intestinal inflammation. RESULTS: The median (25-75 interquartile range) 51Cr-EDTA-IP values of cirrhotic and CCl4-non exposed rats were 0.90% (0.63-1.79) and 0.90% (0.60-1.52) respectively, without significant difference (P = 0.65). Animals from indomethacin group showed 51Cr-EDTA-IP, median 7.3% (5.1-14.7), significantly higher than cirrhotic and CCl4-non exposed rats (P<0.001). CONCLUSION: This study showed the lack of difference between 51Cr-EDTA-intestinal permeability in rats with and without cirrhosis. Further studies are necessary to better clarify the relationship between intestinal permeability and cirrhosis. |
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Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia e Outras Especialidades - IBEPEGE. |
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2010 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-28032010000200014 |
work_keys_str_mv |
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