Yeast species associated with honey: different identification methods
In the present study, three different methods were used to identify yeast isolated from Trás-os-Montes, Portuguese honey. A total of 24 isolates were identified using a partial sequence of the 26S rRNA gene (rDNA), restriction patterns generated from the region spanning the internal transcribed spacers (ITS1 and ITS2) of the 5.8S rRNA gene and the API 20C AUX kit. Nine different yeast species were identified representing six different genera. Among the isolated honey samples, Rhodotorula mucilaginosa, Candida magnoliae and Zygosaccharomyces mellis were the predominant species. Partial sequence of the 26S rDNA yielded the best results in terms of correct identification, followed by-5.8S-ITS analysis. The commercial identification kit API 20C AUX was able to correctly identify only 58% of the isolates. Two new 5.8S-ITS profiles were described, corresponding to Trichosporon mucoides and Candida sorbosivorans.
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Universidad de Córdoba
2010
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oai:scielo:S0004-059220100001000112011-06-08Yeast species associated with honey: different identification methodsCarvalho,C.M.Meirinho,S.Estevinho,M.L.F.Choupina,A. Honey yeast identification 26S rDNA gene 5.8S-ITS region API 20C AUX PCR-RFLP In the present study, three different methods were used to identify yeast isolated from Trás-os-Montes, Portuguese honey. A total of 24 isolates were identified using a partial sequence of the 26S rRNA gene (rDNA), restriction patterns generated from the region spanning the internal transcribed spacers (ITS1 and ITS2) of the 5.8S rRNA gene and the API 20C AUX kit. Nine different yeast species were identified representing six different genera. Among the isolated honey samples, Rhodotorula mucilaginosa, Candida magnoliae and Zygosaccharomyces mellis were the predominant species. Partial sequence of the 26S rDNA yielded the best results in terms of correct identification, followed by-5.8S-ITS analysis. The commercial identification kit API 20C AUX was able to correctly identify only 58% of the isolates. Two new 5.8S-ITS profiles were described, corresponding to Trichosporon mucoides and Candida sorbosivorans.Universidad de CórdobaArchivos de Zootecnia v.59 n.225 20102010-03-01journal articletext/htmlhttp://scielo.isciii.es/scielo.php?script=sci_arttext&pid=S0004-05922010000100011en |
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Carvalho,C.M. Meirinho,S. Estevinho,M.L.F. Choupina,A. |
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Carvalho,C.M. Meirinho,S. Estevinho,M.L.F. Choupina,A. Yeast species associated with honey: different identification methods |
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Carvalho,C.M. Meirinho,S. Estevinho,M.L.F. Choupina,A. |
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Carvalho,C.M. |
| title |
Yeast species associated with honey: different identification methods |
| title_short |
Yeast species associated with honey: different identification methods |
| title_full |
Yeast species associated with honey: different identification methods |
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Yeast species associated with honey: different identification methods |
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Yeast species associated with honey: different identification methods |
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yeast species associated with honey: different identification methods |
| description |
In the present study, three different methods were used to identify yeast isolated from Trás-os-Montes, Portuguese honey. A total of 24 isolates were identified using a partial sequence of the 26S rRNA gene (rDNA), restriction patterns generated from the region spanning the internal transcribed spacers (ITS1 and ITS2) of the 5.8S rRNA gene and the API 20C AUX kit. Nine different yeast species were identified representing six different genera. Among the isolated honey samples, Rhodotorula mucilaginosa, Candida magnoliae and Zygosaccharomyces mellis were the predominant species. Partial sequence of the 26S rDNA yielded the best results in terms of correct identification, followed by-5.8S-ITS analysis. The commercial identification kit API 20C AUX was able to correctly identify only 58% of the isolates. Two new 5.8S-ITS profiles were described, corresponding to Trichosporon mucoides and Candida sorbosivorans. |
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Universidad de Córdoba |
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2010 |
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http://scielo.isciii.es/scielo.php?script=sci_arttext&pid=S0004-05922010000100011 |
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AT carvalhocm yeastspeciesassociatedwithhoneydifferentidentificationmethods AT meirinhos yeastspeciesassociatedwithhoneydifferentidentificationmethods AT estevinhomlf yeastspeciesassociatedwithhoneydifferentidentificationmethods AT choupinaa yeastspeciesassociatedwithhoneydifferentidentificationmethods |
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