Ultra-low temperature conservation of Brazilian Pine embryogenic cultures

DEMARCHI,GRASIELA; STEFENON,VALDIR M.; STEINER,NEUSA; VIEIRA,FELIPE N.; VESCO,LIRIO L. DAL; GUERRA,MIGUEL P.

Ultra-low temperature conservation of Brazilian Pine embryogenic cultures

This study aimed to establish a cryopreservation protocol for embryogenic cultures of A. angustifolia, enabling the ex situ conservation of the species. Embryogenic cultures were established from immature seeds and treated with variations of the cryoprotectant solutions SuDG, SoD and PVS2 prior to immersion in liquid nitrogen. Cell viability was evaluated after 30, 60 and 90 days of re-growth. The highest re-growth without morphological alterations and with normal biochemical composition was obtained with the PVS2 solution with 40 min immersion in ethanol (-20 °C). This procedure opens new horizons for the ex situ conservation of the species genetic.

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Main Authors:	DEMARCHI,GRASIELA, STEFENON,VALDIR M., STEINER,NEUSA, VIEIRA,FELIPE N., VESCO,LIRIO L. DAL, GUERRA,MIGUEL P.
Format:	Digital revista
Language:	English
Published:	Academia Brasileira de Ciências 2014
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000402057
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spelling	oai:scielo:S0001-376520140004020572015-10-27Ultra-low temperature conservation of Brazilian Pine embryogenic culturesDEMARCHI,GRASIELASTEFENON,VALDIR M.STEINER,NEUSAVIEIRA,FELIPE N.VESCO,LIRIO L. DALGUERRA,MIGUEL P. cryopreservation ex situ conservation in vitro culture plant germplasm conservation This study aimed to establish a cryopreservation protocol for embryogenic cultures of A. angustifolia, enabling the ex situ conservation of the species. Embryogenic cultures were established from immature seeds and treated with variations of the cryoprotectant solutions SuDG, SoD and PVS2 prior to immersion in liquid nitrogen. Cell viability was evaluated after 30, 60 and 90 days of re-growth. The highest re-growth without morphological alterations and with normal biochemical composition was obtained with the PVS2 solution with 40 min immersion in ethanol (-20 °C). This procedure opens new horizons for the ex situ conservation of the species genetic.info:eu-repo/semantics/openAccessAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências v.86 n.4 20142014-12-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000402057en10.1590/0001-3765201420130405
institution	SCIELO
collection	OJS
country	Brasil
countrycode	BR
component	Revista
access	En linea
databasecode	rev-scielo-br
tag	revista
region	America del Sur
libraryname	SciELO
language	English
format	Digital
author	DEMARCHI,GRASIELA STEFENON,VALDIR M. STEINER,NEUSA VIEIRA,FELIPE N. VESCO,LIRIO L. DAL GUERRA,MIGUEL P.
spellingShingle	DEMARCHI,GRASIELA STEFENON,VALDIR M. STEINER,NEUSA VIEIRA,FELIPE N. VESCO,LIRIO L. DAL GUERRA,MIGUEL P. Ultra-low temperature conservation of Brazilian Pine embryogenic cultures
author_facet	DEMARCHI,GRASIELA STEFENON,VALDIR M. STEINER,NEUSA VIEIRA,FELIPE N. VESCO,LIRIO L. DAL GUERRA,MIGUEL P.
author_sort	DEMARCHI,GRASIELA
title	Ultra-low temperature conservation of Brazilian Pine embryogenic cultures
title_short	Ultra-low temperature conservation of Brazilian Pine embryogenic cultures
title_full	Ultra-low temperature conservation of Brazilian Pine embryogenic cultures
title_fullStr	Ultra-low temperature conservation of Brazilian Pine embryogenic cultures
title_full_unstemmed	Ultra-low temperature conservation of Brazilian Pine embryogenic cultures
title_sort	ultra-low temperature conservation of brazilian pine embryogenic cultures
description	This study aimed to establish a cryopreservation protocol for embryogenic cultures of A. angustifolia, enabling the ex situ conservation of the species. Embryogenic cultures were established from immature seeds and treated with variations of the cryoprotectant solutions SuDG, SoD and PVS2 prior to immersion in liquid nitrogen. Cell viability was evaluated after 30, 60 and 90 days of re-growth. The highest re-growth without morphological alterations and with normal biochemical composition was obtained with the PVS2 solution with 40 min immersion in ethanol (-20 °C). This procedure opens new horizons for the ex situ conservation of the species genetic.
publisher	Academia Brasileira de Ciências
publishDate	2014
url	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000402057
work_keys_str_mv	AT demarchigrasiela ultralowtemperatureconservationofbrazilianpineembryogeniccultures AT stefenonvaldirm ultralowtemperatureconservationofbrazilianpineembryogeniccultures AT steinerneusa ultralowtemperatureconservationofbrazilianpineembryogeniccultures AT vieirafelipen ultralowtemperatureconservationofbrazilianpineembryogeniccultures AT vescolirioldal ultralowtemperatureconservationofbrazilianpineembryogeniccultures AT guerramiguelp ultralowtemperatureconservationofbrazilianpineembryogeniccultures
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Ultra-low temperature conservation of Brazilian Pine embryogenic cultures

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