EVALUATION OF THE VIABILITY OF PLEUROTUS SPP. STRAINS AFTER LIQUID NITROGEN CRYOPRESERVATION
Viability of 6 mushroom strains of the Pleurotus genus (2 from P. djamor var. djamor, 1 from P. ostreatus var. ostreatus, 2 from P. ostreatus var. columbinus and 1 from P. pulmonarius) after liquid nitrogen cryopreservation (-196º) was evaluated. The contact time for the mycelia of these strains with the cryoprotectant (glycerol) was studied 1, 2 and 3 hours before freezing. We also tested the effect of different times (5, 10 and 15 minutes) and temperatures (30, 45 and 60ºC) of the thawing system for mycelial recovery. The results showed a marked tendency toward faster mycelial recovery when samples were thawed at 30ºC, while at 60ºC no recovery was observed. A change in thawing and contact times with the cryoprotectant did not affect the results significantly, as the thawing temperature and strain employed affected.
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Sociedade Brasileira de Microbiologia
1998
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oai:scielo:S0001-371419980003000091999-02-26EVALUATION OF THE VIABILITY OF PLEUROTUS SPP. STRAINS AFTER LIQUID NITROGEN CRYOPRESERVATIONLara-Herrera,IsabelMata,GerardoGaitán-Hernández,Rigoberto Pleurotus viability liquid nitrogen mushroom Viability of 6 mushroom strains of the Pleurotus genus (2 from P. djamor var. djamor, 1 from P. ostreatus var. ostreatus, 2 from P. ostreatus var. columbinus and 1 from P. pulmonarius) after liquid nitrogen cryopreservation (-196º) was evaluated. The contact time for the mycelia of these strains with the cryoprotectant (glycerol) was studied 1, 2 and 3 hours before freezing. We also tested the effect of different times (5, 10 and 15 minutes) and temperatures (30, 45 and 60ºC) of the thawing system for mycelial recovery. The results showed a marked tendency toward faster mycelial recovery when samples were thawed at 30ºC, while at 60ºC no recovery was observed. A change in thawing and contact times with the cryoprotectant did not affect the results significantly, as the thawing temperature and strain employed affected.info:eu-repo/semantics/openAccessSociedade Brasileira de MicrobiologiaRevista de Microbiologia v.29 n.3 19981998-09-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000300009en10.1590/S0001-37141998000300009 |
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Lara-Herrera,Isabel Mata,Gerardo Gaitán-Hernández,Rigoberto |
spellingShingle |
Lara-Herrera,Isabel Mata,Gerardo Gaitán-Hernández,Rigoberto EVALUATION OF THE VIABILITY OF PLEUROTUS SPP. STRAINS AFTER LIQUID NITROGEN CRYOPRESERVATION |
author_facet |
Lara-Herrera,Isabel Mata,Gerardo Gaitán-Hernández,Rigoberto |
author_sort |
Lara-Herrera,Isabel |
title |
EVALUATION OF THE VIABILITY OF PLEUROTUS SPP. STRAINS AFTER LIQUID NITROGEN CRYOPRESERVATION |
title_short |
EVALUATION OF THE VIABILITY OF PLEUROTUS SPP. STRAINS AFTER LIQUID NITROGEN CRYOPRESERVATION |
title_full |
EVALUATION OF THE VIABILITY OF PLEUROTUS SPP. STRAINS AFTER LIQUID NITROGEN CRYOPRESERVATION |
title_fullStr |
EVALUATION OF THE VIABILITY OF PLEUROTUS SPP. STRAINS AFTER LIQUID NITROGEN CRYOPRESERVATION |
title_full_unstemmed |
EVALUATION OF THE VIABILITY OF PLEUROTUS SPP. STRAINS AFTER LIQUID NITROGEN CRYOPRESERVATION |
title_sort |
evaluation of the viability of pleurotus spp. strains after liquid nitrogen cryopreservation |
description |
Viability of 6 mushroom strains of the Pleurotus genus (2 from P. djamor var. djamor, 1 from P. ostreatus var. ostreatus, 2 from P. ostreatus var. columbinus and 1 from P. pulmonarius) after liquid nitrogen cryopreservation (-196º) was evaluated. The contact time for the mycelia of these strains with the cryoprotectant (glycerol) was studied 1, 2 and 3 hours before freezing. We also tested the effect of different times (5, 10 and 15 minutes) and temperatures (30, 45 and 60ºC) of the thawing system for mycelial recovery. The results showed a marked tendency toward faster mycelial recovery when samples were thawed at 30ºC, while at 60ºC no recovery was observed. A change in thawing and contact times with the cryoprotectant did not affect the results significantly, as the thawing temperature and strain employed affected. |
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Sociedade Brasileira de Microbiologia |
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1998 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000300009 |
work_keys_str_mv |
AT laraherreraisabel evaluationoftheviabilityofpleurotussppstrainsafterliquidnitrogencryopreservation AT matagerardo evaluationoftheviabilityofpleurotussppstrainsafterliquidnitrogencryopreservation AT gaitanhernandezrigoberto evaluationoftheviabilityofpleurotussppstrainsafterliquidnitrogencryopreservation |
_version_ |
1756371777661960192 |